Decoding small peptides: Regulators of plant growth and stress resilience

Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Small peptides (SPs) are pivotal signaling molecules that play essential roles in the precise regulation of plant growth, development, and stress responses. Recent advancements sequencing technologies, bioinformatics approaches, biochemical molecular techniques have significantly enhanced accuracy SP identification, unveiling their diverse biological functions plants. This review provides a comprehensive overview characteristics methodologies for identifying SPs It highlights recent discoveries regarding pathways regulating plant-microbial interactions, as well contributions to resilience under various environmental stresses, including abiotic stress, nutrient deficiencies, biotic challenges. Additionally, we discuss current insights into potential applications outline future research directions aimed at leveraging these enhance adaptation By integrating findings, this lays foundation advancing understanding utilization improve productivity.

Язык: Английский

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Research Progress of Small Plant Peptides on the Regulation of Plant Growth, Development, and Abiotic Stress

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(7), С. 4114 - 4114

Опубликована: Апрель 8, 2024

Small peptides in plants are typically characterized as being shorter than 120 amino acids, with their biologically active variants comprising fewer 20 acids. These instrumental regulating plant growth, development, and physiological processes, even at minimal concentrations. They play a critical role long-distance signal transduction within act primary responders to range of stress conditions, including salinity, alkalinity, drought, high temperatures, cold. This review highlights the crucial roles various small growth resistance abiotic stress, involvement transport. Furthermore, it elaborates regulation hormone biosynthesis. Special emphasis is given functions mechanisms responding aiming provide valuable insights for researchers working on comprehensive study practical application peptides.

Язык: Английский

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Combination of PAMP‐induced peptide signaling and its regulator SpWRKY65 boosts tomato resistance to Phytophthora infestans

The Plant Journal, Год журнала: 2025, Номер 121(6)

Опубликована: Март 1, 2025

SUMMARY Late blight, caused by Phytophthora infestans ( P. ), seriously compromises tomato growth and yield. PAMP‐induced peptides (PIPs) are secreted that act as endogenous elicitors, triggering plant immune responses. Our previous research indicated the exogenous application of PIP1 from Solanum pimpinelifolium L3708, named SpPIP1, enhances resistance to . However, little is known about roles additional family members in In addition, there remains a significant gap understanding receptors SpPIPs transcription factors (TFs) regulate signaling defense, combination TFs defending against pathogens rarely studied. This study demonstrates SpPIP‐LIKE1 (SpPIPL1) also strengthens affecting phenylpropanoid biosynthesis pathway. Both SpPIP1 SpPIPL1 trigger defense responses manner dependent on RLK7L. Tomato plants overexpressing precursors (SpprePIP1 SpprePIPL1) exhibited enhanced expression pathogenesis‐related genes, elevated H 2 O ABA levels, increased lignin accumulation. Notably, SpWRKY65 was identified transcriptional activator SpprePIP1 SpprePIPL1 Disease assays gene analyses revealed overexpression (OEWRKY65) confers , while wrky65 knockout led opposite effect. Intriguingly, transgenic studies showed either spraying OEWRKY65 with or co‐overexpressing further augmented resistance, underscoring potential stacking enhancing disease resistance. summary, this offers new perspectives controlling late blight developing varieties improved The results emphasize an eco‐friendly strategy for crop protection, laying theoretical foundation advancing breeding.

Язык: Английский

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Analysis of the effect of improving drought tolerance via SlGATA17 overexpression on tomato fruit quality

Scientia Horticulturae, Год журнала: 2025, Номер 343, С. 114083 - 114083

Опубликована: Март 1, 2025

Язык: Английский

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DeepPepPI: A deep cross-dependent framework with information sharing mechanism for predicting plant peptide-protein interactions

Expert Systems with Applications, Год журнала: 2024, Номер 252, С. 124168 - 124168

Опубликована: Май 14, 2024

Язык: Английский

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Regulatory Peptide Encoded by the Primary Transcript of miR396a Influences Gene Expression and Root Development in Solanum lycopersicum

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(29), С. 16390 - 16402

Опубликована: Июль 12, 2024

MicroRNAs (miRNAs) are the processing products of primary miRNAs (pri-miRNAs) that regulate expression target genes. Recent studies have demonstrated some pri-miRNAs can encode small peptides (miPEPs) perform significant biological functions. The function miPEPs in tomatoes, an important model horticultural crop, remains to be investigated. Here, we characterized sequence tomato miR396a using 5' RACE and confirmed presence miPEP396a by verifying translational activity start codon. It primarily resides nucleus exert its additionally regulates pri-miR396a, miR396a, Transcriptomic metabolomic analyses showed

Язык: Английский

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SlLTPg1, a tomato lipid transfer protein, positively regulates in response to biotic stresses

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 279, С. 135219 - 135219

Опубликована: Авг. 30, 2024

Язык: Английский

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Molecular Traits of Rapid Alkalinization Factor Family and Functional Analysis of SlRALF2 in Tomato Resistance to Phytophthora infestans

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 29, 2025

Late blight, caused by Phytophthora infestans (P. infestans), poses a significant threat to tomato yield and quality. Traditional disease control strategies rely heavily on frequent applications of chemical pesticides, leading environmental pollution the emergence pesticide-resistant pathogens. This highlights urgent need for environmentally friendly plant technologies. Rapid alkalinization factors (RALFs) are secreted peptides that play crucial roles in defense. Here, 11 SlRALF genes were identified tomato. Bioinformatics analyses conducted characterize them. SlRALF2, which responded significantly P. infection, was chosen investigation. Functional demonstrated SlRALF2 enhances resistance promoting expression level SlPRs modulating key enzyme activities. GO KEGG influenced revealed its involvement several critical pathways. Altogether, our findings establish as pivotal component defense signaling, offering valuable insights crop breeding.

Язык: Английский

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Comparative Transcriptome Analysis of Gene Responses of Salt-Tolerant and Salt-Sensitive Watermelon Cultivars’ Roots to Salt Stress

Plants, Год журнала: 2025, Номер 14(7), С. 1013 - 1013

Опубликована: Март 24, 2025

Salt stress, as a significant adverse consequence of global climate change, severely restricts the yield and quality watermelon. In this study, salt-tolerant cultivar T23 salt-sensitive B2 were subjected to 200 mM NaCl treatment (0 h, 6 24 48 168 h) at three-leaf stage, adaptation mechanisms watermelon roots salt stress systematically investigated phenotypic, physiological, gene transcription levels. Phenotypic observations revealed that inhibited seedling growth, caused leaf curling, induced root yellowing, with damage being significantly more severe in than T23. Compared B2, activities superoxide dismutase (SOD) increased by -7.13%, 169.15%, 34.95%, 84.87%, 39.87% under 0 respectively. h treatment, proline content 4.25%, 14.39%, 110.00% respectively, while showed increases 93.74%, 177.55%, 380.56% corresponding time points. The provided physiological data demonstrate exhibits superior antioxidant osmoregulatory abilities relative B2. transcriptome analysis identified differentially expressed genes (DEGs) between two cultivars showing highest number DEGs exhibited increase h. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment metabolic pathways such plant hormone signal transduction, terpenoid biosynthesis, mitogen-activated protein kinase (MAPK) signaling pathways, transporter activity, regulator activity play important roles response. Furthermore, yeast overexpression experiments preliminarily validated critical tonoplast dicarboxylate ClCG01G010280 NAC factor ClCG05G024110 tolerance. This study provides new molecular insights into tolerance mechanism offers potential genetic resources for breeding varieties.

Язык: Английский

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Constitutive expression of MsPROPEP1 enhances salinity and drought tolerance in alfalfa (Medicago sativa)

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Март 18, 2024

Abstract Plant peptide hormones have various important roles in plant development, defense against pathogens, and tolerance to abiotic stress. However, only a limited number of hormone-like peptides been proven contribute salt drought stress plants other than Arabidopsis . In this study, we present the isolation characterization MsPROPEP1 , propeptide precursor gene obtained from legume pasture Medicago sativa The transcription was found be inducible by NaCl, polyethylene glycol (PEG), abscisic acid (ABA). constitutive expression alfalfa seedlings mitigated restriction on growth imposed either salinity or osmotic raised their sensitivity ABA promoting stomatal closure. addition, synthesized MsPep1 that application enhanced induced NaCl PEG. transgenic plants, many ABA-dependent stress-responsive genes are activated; is known promote peroxidase which plays role reactive oxygen scavenging. Our findings suggest candidate for genetic manipulation species.

Язык: Английский

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