Highly Promiscuous Flavonoid Di-O-glycosyltransferases from Carthamus tinctorius L.

Molecules, Год журнала: 2024, Номер 29(3), С. 604 - 604

Опубликована: Янв. 26, 2024

Safflower (Carthamus tinctorius L.) has been recognized for its medicinal value, but there have limited studies on the glycosyltransferases involved in biosynthesis of flavonoid glycosides from safflower. In this research, we identified two highly efficient O-glycosyltransferases, CtOGT1 and CtOGT2, safflower performing local BLAST alignment. By constructing a prokaryotic expression vector, conducted vitro enzymatic reactions discovered that these enzymes were capable catalyzing two-step O-glycosylation using substrates such as kaempferol, quercetin, eriodictyol. Moreover, they exhibited catalytic activity towards various compounds, including flavones (apigenin, scutellarein), dihydrochalcone (phloretin), isoflavones (genistein, daidzein), flavanones (naringenin, glycyrrhizin), flavanonols (dihydrokaempferol), leading to formation O-glycosides. The broad substrate specificity is noteworthy. This study provides valuable insights into biosynthetic pathways discovery CtOGT2 enhances our understanding processes synthesizing safflower, contributing overall comprehension secondary metabolite plant species.

Язык: Английский

Rational screening of ionic liquids as phase transfer catalysts for aromatic O- and S-glycosidations examplified by acetobromo-alpha-D-galactose with phenolic compound

Journal of Molecular Liquids, Год журнала: 2024, Номер 403, С. 124887 - 124887

Опубликована: Апрель 30, 2024

Язык: Английский