Abstract
Background
Dendrobium
(DEN)
have
been
utilized
as
valuable
medicinal
resource
in
China
used
for
millennia
prevention
and
treatment
of
various
diseases,
particularly
diabetes
via
alteration
their
disordered
gut
microbiota.
Purpose
The
current
study
aimed
to
differentiate
the
quality
different
species
DEN
an
vitro
system
including
microbiota
fermentation
followed
by
antioxidative
hypoglycemic
bioassays.
Material
methods
About
100
g
fresh
from
four
(DEN-5
~
DEN-8)
was
extracted
with
1L
boiling
water
twice
1
h
each
time
centrifugation
obtain
supernatants
aqueous
extract.
10
mg/mL
extract
fermented
fecal
fluid
db/db
mice
12,
24
48
h,
monitoring
changes
total
polysaccharide
polyphenol
contents,
antioxidative,
activities.
Additionally,
level
short
chain
fatty
acids
abundance/diversity
mixture
were
monitored
GC/MS
16S
rRNA,
respectively.
Moreover,
vivo
activities
DEN-5
DEN-8
at
dosage
200
mg/kg
once
daily
14
days
also
evaluated
mice.
Results
8
varied
content
polysaccharides
(0.23–0.61,
w/w)
(0.008–0.023,
w/w).
Based
on
free
radical
scavenging
percentage,
ranked
DEN-7≈DEN-8
>
≈DEN-6.
percentage
enzyme
inhibition,
antihyperglycemic
DEN-5≈DEN-6
DEN-7.
Hypoglycemic
order
Further
correlation
analyses
content,
SCFAs
formation
after
fermentation,
antioxidant
activities,
found
that
1)
contents
positively
correlate
generated
α-glucosidase
inhibitory
capacity,
α-amylase
GLP-1
level;
2)
ABTS,
DPPH,
superoxide
anion
capacity.
Conclusions
Our
first
a
novel
assess
effects
indicated
correlated
effect
while
activity.
Graphical
Biomedicine & Pharmacotherapy,
Год журнала:
2024,
Номер
175, С. 116636 - 116636
Опубликована: Апрель 26, 2024
Renal
interstitial
fibrosis
is
a
pathological
manifestation
of
the
progression
diabetic
kidney
disease
(DKD).
Dendrobium
officinale
polysaccharides
(DOP),
one
major
active
components
officinale,
have
hypoglycemic
and
hypolipidemic
effects
are
used
clinically
to
treat
diabetes.
However,
role
DOP
in
delaying
DKD
remains
unclear.
This
study
aimed
explore
potential
mechanisms
by
which
delays
renal
fibrosis.
Foods,
Год журнала:
2024,
Номер
13(19), С. 3116 - 3116
Опубликована: Сен. 29, 2024
Dendrobium
officinale
flowers
(DOF)
have
previously
been
established
as
a
promising
source
of
natural
antioxidants,
and
it
is
ideally
suited
for
processing
to
prepare
functional
foods
food
additives.
The
precise
extraction
processes
employed,
however,
can
alter
the
composition
antioxidant
properties
resultant
products,
characteristic
compounds
associated
with
active
fractions
prepared
from
DOF
or
their
mechanisms
action
yet
be
reported.
To
clarify
molecular
through
which
these
function
first
time,
chromatography
was
used
separate
extracts,
yielding
five
(Fr.
(a—e)).
Analyses
activity
different
revealed
that
Fr.
(d)
presented
most
robust
bioactivity.
Levels
total
flavonoids
were
then
measured,
revealing
levels
positively
correlated
flavonoid
content.
found
contain
20
in
HPLC-Triple-TOF-MS/MS
analyses.
At
cellular
level,
induce
increases
protective
factors
(SOD
GSH-Px)
while
reducing
reactive
oxygen
species
(ROS),
damage-associated
(MDA,
NO,
TNF-α,
IL-1β,
IL-6),
inducible
nitric
oxide
synthase
(iNOS)
expression
C2C12
cells
had
stimulated
H2O2.
These
data
thus
provided
support
prevention
oxidative
stress
inflammation.
Network
pharmacology
analyses
further
suggested
help
protect
against
its
effects
on
PI3K/Akt-related
signaling
activity.
subsequently
upregulate
PI3K/Akt
pathway-related
proteins,
nuclear
transcription
factor
2
(Nrf2),
heme
oxygenase
1
(HO-1)
addition
suppressing
Kelch-like
epoxide-related
protein
(Keap1)
expression.
In
summary,
suppress
PI3K/Akt/Nrf2
pathway
activation,
ultimately
alleviating
inflammation
predicted
network
approach.
Future
studies
aimed
at
clarifying
mechanistic
will
likely
establish
capable
promoting
health
longevity.
Agronomy,
Год журнала:
2023,
Номер
14(1), С. 69 - 69
Опубликована: Дек. 27, 2023
Dendrobium
officinale
Kimura
et
Migo
is
a
kind
of
herb
with
high
medicinal,
ornamental,
and
commercial
value,
rich
in
polysaccharides.
Polyploid
breeding
an
important
method
for
the
genome
doubling
medicinal
species
to
increase
biomass
polysaccharide
production.
Previous
studies
have
revealed
comparative
transcriptome
analysis
biosynthesis
across
growth
stages
plant
parts,
but
there
been
no
dissecting
such
genes
pathways
tetraploid
D.
officinale.
Therefore,
this
study
aimed
unravel
molecular
mechanisms
content
via
generation
four
transcriptomic
libraries
protocorm-like
bodies
six-month-old
seedlings
both
diploid
plants.
In
study,
total
230,786,618
clean
reads
remained
34.62
Gb
nucleotides
generated;
274,403
unigenes
were
assembled,
which
73.99%
annotated
at
least
one
protein
databases;
17,451
unigenes,
6.35%
all
seven
databases
(NR,
NT,
KO,
Swiss-Prot,
FAM,
GO,
KOG).
Putative
encoding
enzymes
related
biosynthetic
determined.
RT-qPCR
11
randomly
selected
involved
polysaccharides
indicated
consistency
RNA-Seq
data
content.
The
expressions
nine
higher
than
plants,
while
other
two
bifunctional
opposite.
This
has
provided
foundation
subsequent
works
regarding
metabolites
autoploidy
general,
particular.
