Functional characterization of a maize UDP-glucosyltransferase involved in the biosynthesis of flavonoid 7-O-glucosides and di-O-glucosides
Plant Physiology and Biochemistry,
Год журнала:
2025,
Номер
221, С. 109583 - 109583
Опубликована: Янв. 30, 2025
Язык: Английский
Identification and Functional Characterization of Two UDP-Glycosyltransferases Involved in Narcissoside Biosynthesis in Anoectochilus roxburghii
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 19, 2025
Flavonoid
rutinosides,
a
group
of
bioactive
compounds
in
Anoectochilus
roxburghii,
contribute
greatly
to
the
plant's
beneficial
effects
on
human
health.
However,
glycosylation
mechanism
flavonoid
rutinosides
A.
roxburghii
remains
unclear.
In
this
study,
two
efficient
and
selective
glycosyltransferases,
AUTG25
AUTG23,
involved
biosynthesis
narcissoside,
major
rutinoside
were
identified
through
transcriptome
analysis
functional
validation.
could
regioselectively
catalyze
3-O-glucosylation
isorhamnetin
produce
3-O-glucoside,
while
AUTG23
further
6"-O-rhamnosylation
generate
narcissoside.
Both
exhibited
high
positional
sugar
donor
selectivities
catalytic
reaction.
Homology
modeling
site-directed
mutagenesis
showed
that
H20,
E83,
E385,
F143
E280,
E89,
D188,
W327,
D369,
Y191
may
be
critical
for
their
functions.
Transient
expression
Nicotiana
benthamiana
finally
confirmed
possesses
flavonol-3-O-glucosyltransferase
activity
has
flavonol-3-O-glucoside
(1→6)
rhamnosyltransferase
activity.
This
study
clarified
provided
candidate
UDP-dependent
glycosyltransferase
genes
narcissoside
roxburghii.
Язык: Английский
Mining and functional characterization of a flavonoid glucosyltransferase (OtUGT) involved in 7‐O‐glucosylation of apigenin in Ocimum tenuiflorum
The Plant Journal,
Год журнала:
2025,
Номер
121(6)
Опубликована: Март 1, 2025
The
genome
data
of
Ocimum
tenuiflorum
(CIM-Ayu)
were
utilized
for
mining
novel
flavonoid
biosynthesis-related
gene(s).
search
using
the
unannotated
gene
sequences
yielded
an
uncharacterized
glucosyltransferase
(OtUGT),
which
was
selected
characterization.
Its
bioinformatics-based
analysis
predicted
it
to
be
OtUGT.
qRT-PCR
this
OtUGT
indicated
its
highest
expression
in
O.
comparison
other
species.
In
tenuiflorum,
leaf
tissue
compared
trichome,
stem,
flower,
and
root.
found
sensitive
MeJA
light.
Heterologous
protein
induced
BL21(DE3)
strain
Escherichia
coli.
purified
recombinant
used
assay
with
different
sugar
donor
substrates,
whereby
showed
activity
only
apigenin
UDP-α-d-glucose.
product
formed
7-O-glucoside
(apigetrin),
confirmed
through
TLC,
HPLC,
LC-QTOF
mass
spectrometry,
NMR
analyses.
Transient
overexpression
VIGS
appropriately
reflected
terms
alterations
apigetrin
levels
plant.
Apigetrin
content
increased
by
up
2.5-fold
transgenic
Nicotiana
tabacum
lines
generated
transformation
Agrobacterium
tumefaciens
carrying
pBI121-OtUGT
construct.
Hairy
roots
from
explants
A.
rhizogenes
construct
not
advantageous
content.
Since
possesses
high
medicinal
value,
elucidation
biosynthesis
will
enable
economical
production
optimization
plant
or
heterologous
systems
future.
Язык: Английский
Characterization and Engineering of Multifunctional Glycosyltransferase HtUGT73EW3 for the Highly Efficient Synthesis of Flavonoid Mono/di-O-glycosides
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 7, 2025
Glycosylation
modification
is
an
effective
way
to
improve
the
solubility,
stability,
and
bioavailability
of
flavonoids.
In
this
study,
a
multifunctional
flavonoid
glycosyltransferase
HtUGT73EW3
was
identified
from
Helleborus
thibetanus.
exhibited
multisite
selectivity
for
3-,
6-,
7-,
2'-,
3'-,
4'-OH
flavonoids
showed
potent
3/3'-,
3/4'-,
7/4'-di-O-glycosylation
activity.
able
glycosylate
structurally
diverse
aglycones
monoglycosides,
efficient
glycosylation
capacity
toward
structures
modified
with
functional
groups
at
C-3,
C-7,
C-8,
C-4'
positions.
Notably,
mutation
Gln85
Leu
greatly
enhanced
its
catalytic
activity,
enabling
not
only
conversion
steroids
terpenoids,
but
also
improved
utilization
UDP-sugars.
Furthermore,
Q85L
I94A
variants
were
found
catalyze
specific
7,4'-
3,4'-di-O-glycosylation,
respectively.
A
cost-effective
one-pot
synthetic
reaction
established
by
coupling
AtSuSy
HtUGT73EW3,
gram-scale
synthesis
4'-O-glucoside
3,4'-/7,4'-di-O-glucoside
achieved
fed-batch
strategy.
Язык: Английский
Celebrating Fifty Years of AGRO and a Fruitful Partnership with JAFC
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(22), С. 12295 - 12296
Опубликована: Июнь 5, 2024
ADVERTISEMENT
RETURN
TO
ISSUEEditorialNEXTCelebrating
Fifty
Years
of
AGRO
and
a
Fruitful
Partnership
with
JAFCLaura
L.
McConnell*Laura
McConnellRegulatory
Scientific
Affairs,
Bayer
U.S.
LLC,
Crop
Science
Division,
St.
Louis,
Missouri
63017,
United
States*[email
protected]More
by
Laura
McConnellhttps://orcid.org/0000-0001-6142-0656,
Thomas
C.
SparksThomas
SparksAgrilucent
Morro
Bay,
California
93442,
StatesMore
Sparkshttps://orcid.org/0000-0002-0526-7594,
Jeanette
M.
Van
EmonJeanette
EmonEVE
Sciences,
2590
Windmill
Ln.
#356,
Henderson,
Nevada
89074,
Emon,
F.
HofmannThomas
HofmannChair
Food
Chemistry
Molecular
Sensory
Science,
Technical
University
Munich,
Lise-Meitner-Strasse
34,
D-85354
Freising-Weihenstephan,
GermanyMore
Hofmannhttps://orcid.org/0000-0003-4057-7165Cite
this:
J.
Agric.
Chem.
2024,
72,
22,
12295–12296Publication
Date
(Web):June
5,
2024Publication
History
Received10
May
2024Published
online5
June
inissue
5
2024https://pubs.acs.org/doi/10.1021/acs.jafc.4c04112https://doi.org/10.1021/acs.jafc.4c04112editorialACS
PublicationsCopyright
©
Published
2024
American
Chemical
Society.
This
publication
is
available
under
these
Terms
Use.
Request
reuse
permissions
free
to
access
through
this
site.
Learn
MoreArticle
Views-Altmetric-Citations-LEARN
ABOUT
THESE
METRICSArticle
Views
are
the
COUNTER-compliant
sum
full
text
article
downloads
since
November
2008
(both
PDF
HTML)
across
all
institutions
individuals.
These
metrics
regularly
updated
reflect
usage
leading
up
last
few
days.Citations
number
other
articles
citing
article,
calculated
Crossref
daily.
Find
more
information
about
citation
counts.The
Altmetric
Attention
Score
quantitative
measure
attention
that
research
has
received
online.
Clicking
on
donut
icon
will
load
page
at
altmetric.com
additional
details
score
social
media
presence
for
given
article.
how
calculated.
Share
Add
toView
InAdd
Full
Text
ReferenceAdd
Description
ExportRISCitationCitation
abstractCitation
referencesMore
Options
onFacebookTwitterWechatLinked
InRedditEmail
(867
KB)
Get
e-AlertscloseSUBJECTS:Agriculture,Agrochemicals,Food,Pest
control,Safety
e-Alerts
Язык: Английский