Celebrating Fifty Years of AGRO and a Fruitful Partnership with JAFC DOI Creative Commons
Laura L. McConnell, Thomas C. Sparks,

Jeanette M. Van Emon

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(22), С. 12295 - 12296

Опубликована: Июнь 5, 2024

ADVERTISEMENT RETURN TO ISSUEEditorialNEXTCelebrating Fifty Years of AGRO and a Fruitful Partnership with JAFCLaura L. McConnell*Laura McConnellRegulatory Scientific Affairs, Bayer U.S. LLC, Crop Science Division, St. Louis, Missouri 63017, United States*[email protected]More by Laura McConnellhttps://orcid.org/0000-0001-6142-0656, Thomas C. SparksThomas SparksAgrilucent Morro Bay, California 93442, StatesMore Sparkshttps://orcid.org/0000-0002-0526-7594, Jeanette M. Van EmonJeanette EmonEVE Sciences, 2590 Windmill Ln. #356, Henderson, Nevada 89074, Emon, F. HofmannThomas HofmannChair Food Chemistry Molecular Sensory Science, Technical University Munich, Lise-Meitner-Strasse 34, D-85354 Freising-Weihenstephan, GermanyMore Hofmannhttps://orcid.org/0000-0003-4057-7165Cite this: J. Agric. Chem. 2024, 72, 22, 12295–12296Publication Date (Web):June 5, 2024Publication History Received10 May 2024Published online5 June inissue 5 2024https://pubs.acs.org/doi/10.1021/acs.jafc.4c04112https://doi.org/10.1021/acs.jafc.4c04112editorialACS PublicationsCopyright © Published 2024 American Chemical Society. This publication is available under these Terms Use. Request reuse permissions free to access through this site. Learn MoreArticle Views-Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com additional details score social media presence for given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail (867 KB) Get e-AlertscloseSUBJECTS:Agriculture,Agrochemicals,Food,Pest control,Safety e-Alerts

Язык: Английский

Functional characterization of a maize UDP-glucosyltransferase involved in the biosynthesis of flavonoid 7-O-glucosides and di-O-glucosides DOI
Julieta Petrich, Clarisa E. Álvarez,

Lina Gómez Cano

и другие.

Plant Physiology and Biochemistry, Год журнала: 2025, Номер 221, С. 109583 - 109583

Опубликована: Янв. 30, 2025

Язык: Английский

Процитировано

0

Identification and Functional Characterization of Two UDP-Glycosyltransferases Involved in Narcissoside Biosynthesis in Anoectochilus roxburghii DOI
Xuhui He, Hongrui Wang, Mingyue Zhang

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

Flavonoid rutinosides, a group of bioactive compounds in Anoectochilus roxburghii, contribute greatly to the plant's beneficial effects on human health. However, glycosylation mechanism flavonoid rutinosides A. roxburghii remains unclear. In this study, two efficient and selective glycosyltransferases, AUTG25 AUTG23, involved biosynthesis narcissoside, major rutinoside were identified through transcriptome analysis functional validation. could regioselectively catalyze 3-O-glucosylation isorhamnetin produce 3-O-glucoside, while AUTG23 further 6"-O-rhamnosylation generate narcissoside. Both exhibited high positional sugar donor selectivities catalytic reaction. Homology modeling site-directed mutagenesis showed that H20, E83, E385, F143 E280, E89, D188, W327, D369, Y191 may be critical for their functions. Transient expression Nicotiana benthamiana finally confirmed possesses flavonol-3-O-glucosyltransferase activity has flavonol-3-O-glucoside (1→6) rhamnosyltransferase activity. This study clarified provided candidate UDP-dependent glycosyltransferase genes narcissoside roxburghii.

Язык: Английский

Процитировано

0

Mining and functional characterization of a flavonoid glucosyltransferase (OtUGT) involved in 7‐O‐glucosylation of apigenin in Ocimum tenuiflorum DOI
Saumya Shah,

Shubhra Rastogi,

Md. Qussen Akhtar

и другие.

The Plant Journal, Год журнала: 2025, Номер 121(6)

Опубликована: Март 1, 2025

The genome data of Ocimum tenuiflorum (CIM-Ayu) were utilized for mining novel flavonoid biosynthesis-related gene(s). search using the unannotated gene sequences yielded an uncharacterized glucosyltransferase (OtUGT), which was selected characterization. Its bioinformatics-based analysis predicted it to be OtUGT. qRT-PCR this OtUGT indicated its highest expression in O. comparison other species. In tenuiflorum, leaf tissue compared trichome, stem, flower, and root. found sensitive MeJA light. Heterologous protein induced BL21(DE3) strain Escherichia coli. purified recombinant used assay with different sugar donor substrates, whereby showed activity only apigenin UDP-α-d-glucose. product formed 7-O-glucoside (apigetrin), confirmed through TLC, HPLC, LC-QTOF mass spectrometry, NMR analyses. Transient overexpression VIGS appropriately reflected terms alterations apigetrin levels plant. Apigetrin content increased by up 2.5-fold transgenic Nicotiana tabacum lines generated transformation Agrobacterium tumefaciens carrying pBI121-OtUGT construct. Hairy roots from explants A. rhizogenes construct not advantageous content. Since possesses high medicinal value, elucidation biosynthesis will enable economical production optimization plant or heterologous systems future.

Язык: Английский

Процитировано

0

Characterization and Engineering of Multifunctional Glycosyltransferase HtUGT73EW3 for the Highly Efficient Synthesis of Flavonoid Mono/di-O-glycosides DOI
Yiping Guo,

Zhu Jie,

Xiong Liao

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 7, 2025

Glycosylation modification is an effective way to improve the solubility, stability, and bioavailability of flavonoids. In this study, a multifunctional flavonoid glycosyltransferase HtUGT73EW3 was identified from Helleborus thibetanus. exhibited multisite selectivity for 3-, 6-, 7-, 2'-, 3'-, 4'-OH flavonoids showed potent 3/3'-, 3/4'-, 7/4'-di-O-glycosylation activity. able glycosylate structurally diverse aglycones monoglycosides, efficient glycosylation capacity toward structures modified with functional groups at C-3, C-7, C-8, C-4' positions. Notably, mutation Gln85 Leu greatly enhanced its catalytic activity, enabling not only conversion steroids terpenoids, but also improved utilization UDP-sugars. Furthermore, Q85L I94A variants were found catalyze specific 7,4'- 3,4'-di-O-glycosylation, respectively. A cost-effective one-pot synthetic reaction established by coupling AtSuSy HtUGT73EW3, gram-scale synthesis 4'-O-glucoside 3,4'-/7,4'-di-O-glucoside achieved fed-batch strategy.

Язык: Английский

Процитировано

0

Celebrating Fifty Years of AGRO and a Fruitful Partnership with JAFC DOI Creative Commons
Laura L. McConnell, Thomas C. Sparks,

Jeanette M. Van Emon

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(22), С. 12295 - 12296

Опубликована: Июнь 5, 2024

ADVERTISEMENT RETURN TO ISSUEEditorialNEXTCelebrating Fifty Years of AGRO and a Fruitful Partnership with JAFCLaura L. McConnell*Laura McConnellRegulatory Scientific Affairs, Bayer U.S. LLC, Crop Science Division, St. Louis, Missouri 63017, United States*[email protected]More by Laura McConnellhttps://orcid.org/0000-0001-6142-0656, Thomas C. SparksThomas SparksAgrilucent Morro Bay, California 93442, StatesMore Sparkshttps://orcid.org/0000-0002-0526-7594, Jeanette M. Van EmonJeanette EmonEVE Sciences, 2590 Windmill Ln. #356, Henderson, Nevada 89074, Emon, F. HofmannThomas HofmannChair Food Chemistry Molecular Sensory Science, Technical University Munich, Lise-Meitner-Strasse 34, D-85354 Freising-Weihenstephan, GermanyMore Hofmannhttps://orcid.org/0000-0003-4057-7165Cite this: J. Agric. Chem. 2024, 72, 22, 12295–12296Publication Date (Web):June 5, 2024Publication History Received10 May 2024Published online5 June inissue 5 2024https://pubs.acs.org/doi/10.1021/acs.jafc.4c04112https://doi.org/10.1021/acs.jafc.4c04112editorialACS PublicationsCopyright © Published 2024 American Chemical Society. This publication is available under these Terms Use. Request reuse permissions free to access through this site. Learn MoreArticle Views-Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com additional details score social media presence for given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail (867 KB) Get e-AlertscloseSUBJECTS:Agriculture,Agrochemicals,Food,Pest control,Safety e-Alerts

Язык: Английский

Процитировано

0