Microbial upcycling of agricultural cabbage waste into novel dietary carotenoid, phytoene: Advancing circular economy from waste to natural products

Energy & Environment, Год журнала: 2025, Номер unknown

Опубликована: Янв. 24, 2025

Growing concerns over food shortages and climate-related environmental issues have increased interest in technologies that recycle renewable resources add value to byproducts. Here, we report microbial upcycling of agricultural cabbage waste (ACW) into phytoene, a novel dietary carotenoid through rational metabolic engineering. To make the base strain, ability produce phytoene was initially tested various E. coli strains harboring crtB crtE genes are essential for synthesis. Next, mevalonate biosynthetic pathway gene cluster introduced further increase carbon flux toward production. Finally, poly(3-hydroxybutyrate) [P3HB] biosynthesis phytoene-producing strain storage capacity enhance photostability phytoene. The final engineered PHY4 produced 9.51 mg/L (5.6 mg/g DCW) with productivity 0.4 mg/L/h from 1% ACW hydrolysates, resulting approximately 22.1-fold titer compared PHY1 (0.43 ± 0.06 mg/L) 10 g/L glucose. Moreover, exhibited three-fold improvement strategies reported here will be useful high-valued natural products.

Язык: Английский

Valorizing agricultural waste: Utilizing corn plant leftover to grow yeast biomass, as a potential source of sustainable protein

Future Foods, Год журнала: 2024, Номер unknown, С. 100523 - 100523

Опубликована: Дек. 1, 2024

Язык: Английский