Early Weaning Inhibits Intestinal Stem Cell Expansion to Disrupt the Intestinal Integrity of Duroc Piglets via Regulating the Keap1/Nrf2 Signaling DOI Creative Commons

Ying‐chao Qin,

Chenglong Jin,

Ting-cai Hu

и другие.

Antioxidants, Год журнала: 2024, Номер 13(10), С. 1188 - 1188

Опубликована: Сен. 30, 2024

There are different stress resistance among breeds of pigs. Changes in intestinal stem cells (ISCs) still unclear various piglets after early weaning. In the current study, Taoyuan Black and Duroc were slaughtered at 21 days age (early weaning day) 24 (3 weaning) for 10 each group. The results showed that rate ISC-driven epithelial renewal local pigs hardly changed 3 days. However, significantly reduced weight duodenum jejunum piglets. Meanwhile, jejunal villus height, tight junction-related proteins (ZO-1, Occludin, Claudin1), as well trans-epithelial electrical (TEER) values, down-regulated Moreover, compared with Unweaned piglets, numbers Olfm4

Язык: Английский

PXR Activation Relieves Deoxynivalenol‐Induced Liver Oxidative Stress Via Malat1 LncRNA m6A Demethylation DOI Creative Commons
Yue Feng, Jiakun Shen, Zishen Lin

и другие.

Advanced Science, Год журнала: 2024, Номер 11(25)

Опубликована: Апрель 24, 2024

Abstract Deoxynivalenol (DON) is a prevalent toxin causing severe liver damage through hepatocellular oxidative stress. However, the underlying mechanisms and effective therapeutic approaches remain unknown. Here, unique role of xenobiotic metabolism factor pregnane X receptor (PXR) in mediating DON‐induced stress investigated. Treatment with PXR agonist 3‐indole‐propionic acid (IPA) alleviates injury both vitro vivo. Mechanistically, it discovered for first time that IPA directly transactivates m 6 A demethylase FTO expression, leading to site‐specific demethylation decreased abundance YTHDC1‐bound Malat1 lncRNA at single‐nucleotide resolution. The diminished modification reduces its stability augments antioxidant pathways governed by NRF2, consequently mitigating injury. Furthermore, knockout mice exhibit injury, emphasizing Collectively, findings establish PXR‐mediated A‐dependent expression determines hepatocyte via FTO, providing valuable insights into potential offers strategies treatment.

Язык: Английский

Процитировано

13

Lithocholic acid attenuates DON-induced inflammatory responses via epigenetic regulation of DUSP5 and TRAF5 in porcine intestinal epithelial cells DOI Creative Commons
Shiqi Wang,

Xiaoxu Peng,

Qi Zhu

и другие.

Frontiers in Veterinary Science, Год журнала: 2025, Номер 12

Опубликована: Фев. 28, 2025

Deoxynivalenol (DON) is the most common mycotoxin that frequently contaminates human food and animal feed, resulting in intestinal diseases systemic immunosuppression. Lithocholic acid (LCA) exhibits various pharmacological activities. RNA-seq ChIP-qPCR analysis were used current study to investigate protective mechanism of LCA for DON-induced inflammatory Responses via Epigenetic Regulation DUSP5 TRAF5 porcine ileal epithelial cell lines (IPI-2I) cells. The IPI-2I cells treated with vehicle group, 250 ng/mL DON, 20 μmol/L LCA, DON+ 24 h could induce By analyzing transcriptional profiles DON LCA-treated IPI-2I, we observed significant changes Further up-and down-regulated differential genes revealed enrichment pathways closely related inflammation apoptosis, such as MAPK signaling pathway, IL17 Wnt pathway. An upregulated ( p < 0.05) relative mRNA expression level RAP1B, GDNF, FGF2, IL1R1, RAPGEF2, DUSP5, TGFB3, CACNA1G, TEK RPS6KA2 noted exposed DON. DON-exposed dramatically enhanced histone marks associated activation, H3K9ac, H3K18ac, H3K27ac, H3K4me1, H3K9bhb, H3K18bhb Pol-II Ser5 at enhancers TRAF5. Overall, our findings provide a theoretical basis understanding action attenuating injury better potential treatment or prevention mycotoxin-associated swine production.

Язык: Английский

Процитировано

0

Magnesium-based implants accelerate femoral fracture healing through promoting histone lactylation-mediated osteoclastogenesis inhibition DOI Creative Commons

Junyi Shen,

Yilun Pei,

Shangying Bai

и другие.

Life Sciences, Год журнала: 2025, Номер 372, С. 123639 - 123639

Опубликована: Апрель 17, 2025

To investigate the molecular mechanisms by which magnesium (Mg)-based implants, specifically Mg-containing intramedullary nails (Mg-IMNs), promote femoral fracture healing. Rats with fractures were treated Mg-IMNs. In vitro experiments conducted to assess impact of Mg2+ on osteoclastogenesis and histone lactylation. Histological analysis, Western blotting, qRT-PCR employed evaluate osteoclast maturation pathways involved. vivo, lactate was administered replicate Mg-IMN effects, production inhibited observe potential reversal effects. Mg-IMNs significantly enhanced healing inhibiting osteoclastogenesis. promoted intracellular production, leading lactylation, suppressed downregulating NFATc1. The P300/H3K18LA/HDAC1 pathway identified as a key mediator in this process. Additionally, administration mimicked effects Mg-IMNs, while blocking reversed these This study uncovers novel mechanism promotes through lactylation-mediated inhibition These findings offer new therapeutic strategies for enhancing repair via epigenetic regulation.

Язык: Английский

Процитировано

0

The regulatory role of protein lactylation in various diseases: Special focus on the regulatory role of non-histone lactylation DOI
Peipei Wang,

Kexin Lin,

Degao Huang

и другие.

Gene, Год журнала: 2025, Номер unknown, С. 149595 - 149595

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0

Early Weaning Inhibits Intestinal Stem Cell Expansion to Disrupt the Intestinal Integrity of Duroc Piglets via Regulating the Keap1/Nrf2 Signaling DOI Creative Commons

Ying‐chao Qin,

Chenglong Jin,

Ting-cai Hu

и другие.

Antioxidants, Год журнала: 2024, Номер 13(10), С. 1188 - 1188

Опубликована: Сен. 30, 2024

There are different stress resistance among breeds of pigs. Changes in intestinal stem cells (ISCs) still unclear various piglets after early weaning. In the current study, Taoyuan Black and Duroc were slaughtered at 21 days age (early weaning day) 24 (3 weaning) for 10 each group. The results showed that rate ISC-driven epithelial renewal local pigs hardly changed 3 days. However, significantly reduced weight duodenum jejunum piglets. Meanwhile, jejunal villus height, tight junction-related proteins (ZO-1, Occludin, Claudin1), as well trans-epithelial electrical (TEER) values, down-regulated Moreover, compared with Unweaned piglets, numbers Olfm4

Язык: Английский

Процитировано

0