Identification of Polyamines in Human Milk, Formula Milk, and Other Dairy Products: a Special Method Based on Precolumn Derivatization, Double-Florisil Purification, and Gas Chromatography–Triple Quadrupole Mass Spectrometry DOI
Li Wang, Yating Sun,

Linjing Dong

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

Polyamines in human milk are essential for the rapid growth and development of neonates infants. However, concentrations polyamines have not been well characterized special methods detecting milk, formula or other dairy products remain largely unavailable to date. In this study, an accurate sensitive method based on trifluoroacetic acid anhydride (TFAA) derivatization, double solid-phase extraction (SPE) purification, gas chromatography-triple quadrupole mass spectrometry (GC-QqQ MS) was established evaluation products. High coefficients determination internal calibration curves were obtained (R2 > 0.99). Low limits detection (5.0, 1.0, 0.5 nmol/dL) quantification (10.0, 3.0, 1.0 putrescine, spermidine, spermine, respectively, also achieved. The precision (expressed as relative standard deviation, RSD < 9.0%) recovery rates (78.3-108.4%) satisfactory. Spermidine spermine found significantly higher than those commercial cow's milk. difference polyamine content infant should be paid attention to, particularly when is used a complete replacement

Язык: Английский

Harnessing Microalgae as Sustainable Cell Factories for Polyamine-Based Nanosilica for Biomedical Applications DOI Creative Commons
Sik Yoon, Boon‐Huat Bay, Ken Matsumoto

и другие.

Molecules, Год журнала: 2025, Номер 30(8), С. 1666 - 1666

Опубликована: Апрель 8, 2025

Microalgae are microscopic unicellular organisms that inhabit marine, freshwater, and moist terrestrial ecosystems. The vast number diversity of microalgal species provide a significant reservoir biologically active compounds, highly promising for biomedical applications. Diatoms eukaryotic algae belonging to the class Bacillariophyceae. They possess intricately structured silica-based cell walls, which contain long-chain polyamines play important roles in formation silica. Long-chain uncommon found only produce biosilica. Diatomite, is marine sediment remains silica skeleton diatoms, could be an abundant source biogenic can easily converted particles. This concise review focuses on biofabrication polyamine-based nanosilica from diatoms highlights possibility utilizing diatom biosilica as nanocarrier drug siRNA delivery, bioimaging, bone tissue engineering. challenges may affect production, including environmental stresses climate change, discussed together with prospect increasing diatom-based production desired nanostructures via genetic manipulation.

Язык: Английский

Процитировано

1

Metabolomics of rapeseed (Brassica napus var. oleifera Del.) sprouts obtained with or without salinity from progeny seeds of mother plants grown in presence or absence of salinity DOI Creative Commons

Filippo Sevi,

Beatrice Falcinelli, Sarah Frusciante

и другие.

Journal of Agriculture and Food Research, Год журнала: 2025, Номер unknown, С. 101649 - 101649

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Enrichment of the nutritional value of pea flour milling fractions through fermentation DOI Creative Commons

Tina Šaula,

Blaž Cigić,

Polona Jamnik

и другие.

Food Chemistry, Год журнала: 2025, Номер 476, С. 143303 - 143303

Опубликована: Фев. 13, 2025

In this work, pea flour and two milling fractions obtained by industrial-scale air classification were characterized fermented Lactiplantibacillus plantarum to increase their nutritional value. Scanning electron microscopy chemical analysis revealed major differences in the morphology composition of flours. Protein-rich (43.7 %) fraction exhibits a few-fold higher mineral, spermidine (290 μg/g), but also phytate (20.4 mg/g) content compared starch-rich fraction. Flour type inoculum majorly influenced product. spontaneously flours, biogenic amines accumulated up 6.6 mg/g, which was main drawback besides large variations between batches, as confirmed metagenomic analysis. Higher contents lactic acid, free amino groups formed proteolysis gamma-aminobutyric acid determined inoculated fermentations protein rich fraction, whereas relative bioavailability minerals found reduced 42 %.

Язык: Английский

Процитировано

0

Identification of Polyamines in Human Milk, Formula Milk, and Other Dairy Products: a Special Method Based on Precolumn Derivatization, Double-Florisil Purification, and Gas Chromatography–Triple Quadrupole Mass Spectrometry DOI
Li Wang, Yating Sun,

Linjing Dong

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

Polyamines in human milk are essential for the rapid growth and development of neonates infants. However, concentrations polyamines have not been well characterized special methods detecting milk, formula or other dairy products remain largely unavailable to date. In this study, an accurate sensitive method based on trifluoroacetic acid anhydride (TFAA) derivatization, double solid-phase extraction (SPE) purification, gas chromatography-triple quadrupole mass spectrometry (GC-QqQ MS) was established evaluation products. High coefficients determination internal calibration curves were obtained (R2 > 0.99). Low limits detection (5.0, 1.0, 0.5 nmol/dL) quantification (10.0, 3.0, 1.0 putrescine, spermidine, spermine, respectively, also achieved. The precision (expressed as relative standard deviation, RSD < 9.0%) recovery rates (78.3-108.4%) satisfactory. Spermidine spermine found significantly higher than those commercial cow's milk. difference polyamine content infant should be paid attention to, particularly when is used a complete replacement

Язык: Английский

Процитировано

0