Life, Год журнала: 2023, Номер 13(5), С. 1109 - 1109
Опубликована: Апрель 29, 2023
In aging laying hens, reproductive changes reduce egg quality.
Язык: Английский
Engineering Microbiology, Год журнала: 2024, Номер 4(3), С. 100154 - 100154
Опубликована: Май 29, 2024
Terpenoids are widely used as medicines, flavors, and biofuels. However, the use of these natural products is largely restricted by their low abundance in native plants. Fortunately, heterologous biosynthesis terpenoids microorganisms offers an alternative sustainable approach for efficient production. Various genome-editing technologies have been developed microbial strain construction. Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9) most commonly system owing to its outstanding efficiency convenience genome editing. In this review, basic principles CRISPR-Cas9 systems briefly introduced applications engineering bacteria production plant-derived summarized. The aim review provide overview current developments CRISPR-Cas9-based bacterial engineering, concluding with perspectives on challenges opportunities technologies.
Язык: Английский
Процитировано
5
ACS Synthetic Biology, Год журнала: 2025, Номер unknown
Опубликована: Март 4, 2025
Bacillus subtilis is the model Gram-positive and industrial chassis bacterium; it has blossomed as a robust promising host for enzyme, biochemical, or bioflocculant production. However, synthetic biology metabolic engineering technologies of B. have lagged behind most widely used Saccharomyces cerevisiae Escherichia coli. CRISPR (an acronym clustered regularly interspaced short palindromic repeats) enables efficient, site-specific, programmable DNA cleavage, which revolutionized manner genome editing. In 2016, technology was first introduced into been intensely upgraded since then. this Review, we discuss recently developed key additions to toolkit design in with gene editing, transcriptional regulation, enzyme modulation. Second, advances efficient biochemicals proteins are discussed. Finally, conclude perspectives on challenges opportunities CRISPR-based biotechnology subtilis, wishing that can be comparable traditional microorganisms such E. coli S. someday soon.
Язык: Английский
Процитировано
0
Cogent Food & Agriculture, Год журнала: 2025, Номер 11(1)
Опубликована: Май 27, 2025
Язык: Английский
Процитировано
0
Life, Год журнала: 2023, Номер 13(5), С. 1109 - 1109
Опубликована: Апрель 29, 2023
In aging laying hens, reproductive changes reduce egg quality.
Язык: Английский
Процитировано
4