Food & Function,
Год журнала:
2024,
Номер
15(21), С. 10698 - 10716
Опубликована: Янв. 1, 2024
Inflammatory
bowel
disease
(IBD)
poses
persistent
challenges
due
to
its
chronic
and
recurrent
nature,
exacerbated
by
the
unsatisfactory
outcomes
of
traditional
treatment
approaches.
In
this
study,
we
developed
a
dietary
supplement,
selenium-enriched
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 5, 2025
The
molecular
structures
and
immunomodulatory
effects
of
mycelial
polysaccharides
(MPSs)
exopolysaccharides
(EPSs)
from
wild-type
(WT)
Monascus
purpureus
M9
its
α-1,3-glucan
synthase
gene
(ags1)
mutant
strains
(Δags1:
deletion;
ags1OE:
overexpression)
were
compared
in
this
study.
Mycelial
aggregation
was
reduced
Δags1
increased
ags1OE,
confirming
the
positive
regulatory
role
α-1,3-glucan.
EPS
content
significantly
higher
lower
whereas
MPS
contents
showed
opposite
trend.
relative
galactomannan
MPSs
EPSs
following
order:
ags1OE
<
WT
ags1OE.
In
RAW
264.7
cells,
both
enhanced
cell
viability
promoted
NO
cytokine
(interleukin-6
tumor
necrosis
factor-α)
secretion.
Notably,
Δags1-MPS
ags1OE-EPS
exhibited
to
those
WT,
likely
due
their
contents.
Future Foods,
Год журнала:
2024,
Номер
10, С. 100439 - 100439
Опубликована: Авг. 30, 2024
Edible
insects
are
a
promising
source
of
bioactive
compounds
with
diverse
health
benefits.
This
study
investigated
the
immunomodulatory
properties
polysaccharides
isolated
from
Protaetia
brevitarsis
seulensis
larvae
(PBSL).
Polysaccharides
were
fractionated
using
molecular
weight
cutoff
membrane,
and
among
these
fractions,
higher
fraction
(H-PBSL,
666.84
kDa)
demonstrated
significant
activity.
Methylation
NMR
structural
analyses
revealed
H-PBSL
to
be
an
acidic
heteroglycan
complex
structure,
consisting
backbone
repeating
(1→4)-linked
α-glucan
units
highly
branched
at
O-6
positions
T-α-Glcp,
T-α-GlcpA,
T-β-Galp.
The
effects
evaluated
RAW264.7
macrophage
cells.
treatment
enhanced
phagocytic
activity
increased
expression
immune
modulators
activation
surface
molecules.
In
addition,
activated
NF-κB
signaling
pathway,
evidenced
by
phosphorylation
IκB
NF-κB,
translocation
cytosol
nucleus.
Moreover,
inhibition
Toll-like
receptor
4
(TLR4)
reduced
H-PBSL-induced
nitric
oxide
synthesis,
indicating
TLR4′s
pivotal
role
in
H-PBSL-mediated
activation.
These
findings
highlight
potential
as
novel
agent
derived
edible
insect,
possible
applications
functional
foods
nutraceuticals.
