Plasmonics-Enhanced Dual-Modal Colorimetric and Photothermal Lateral Flow Immunoassay Using Gold Nanocages

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 18, 2025

Lateral flow immunoassays (LFIA) are widely recognized as cost-effective point-of-care diagnostic tools (POCT) for infectious disease diagnosis. Despite their widespread use, traditional colorimetric LFIAs, which rely on gold nanospheres (GNP), constrained by a limited sensitivity. To overcome this challenge, we have engineered nanocages (GNCs) with optimized core-to-shell morphologies, achieving significant amplification of both and photothermal LFIA readout signals. The distinctive morphology GNCs, featuring adjustable gap thicknesses, enables fine-tuning the localized surface plasmon resonance (LSPR) peak across broad spectral range from 600 to 1200 nm. Among GNC morphologies evaluated, (GNC-4), characterized its larger size maximal thickness, exhibited superior color brightness enhanced efficiency compared other GNP. performance GNC-4 enabled detection influenza A (H1N1), used model analyte, limit (LOD) 1.8 ng/mL via analysis 1.51 pg/mL using LFIA. Compared GNP-based detection, sensitivity GNC-4-based was 7-fold, while showed an improvement over 8000-fold. By incorporating portable smartphone-based platform, our dual-modal exhibits high sensitivity, practicality in detecting H1N1 spiked saliva samples, long-term stability five months, making it promising tool potential diagnosing pathogens.

Язык: Английский

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Bidirectional “Win–Win”: Asymmetrical Nanobowl-Coupled Aggregation-Induced Emissive Nanosilicon-Enhanced Immunochromatographic Strips for the Ultrasensitive Detection of Salmonella typhimurium

Analytical Chemistry, Год журнала: 2024, Номер 96(45), С. 18204 - 18213

Опубликована: Ноя. 1, 2024

The lack of nanoprobes with an efficient signal response and overlook cooperation between can be responsible for the unsatisfactory analytical performance immunochromatographic strips (ITSs). Herein, asymmetrical nanobowl-confined innumerable gold nanoparticles (AuNPs) (AuNPs@AFRNBs) to enhance light absorption are developed quenching fluorescence aggregation-induced emissive (AIE) nanosilicons, which is used construction a bidirectional complementary-enhanced ITS (BC-ITS) ultrasensitively detect

Язык: Английский

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Metal self-reducing triggered green etching for the CuO@Ag composite synthesis and its application in high-sensitive lateral flow immunoassay

Chemical Engineering Journal, Год журнала: 2024, Номер 498, С. 155341 - 155341

Опубликована: Сен. 6, 2024

Язык: Английский

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Highly sensitive detection of carbendazim in agricultural products using colorimetric and photothermal lateral flow immunoassay based on plasmonic gold nanostars

Talanta, Год журнала: 2024, Номер 281, С. 126891 - 126891

Опубликована: Сен. 14, 2024

Язык: Английский

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Optical lateral flow immune assay technology for body fluid sensing

Chinese Chemical Letters, Год журнала: 2025, Номер unknown, С. 110967 - 110967

Опубликована: Фев. 1, 2025

Язык: Английский

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Recent advances in lateral flow immunoassay based on sandwich format for whole-cell pathogen detection

Coordination Chemistry Reviews, Год журнала: 2025, Номер 533, С. 216538 - 216538

Опубликована: Фев. 21, 2025

Язык: Английский

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“Integrated Stacked” Design “Nanobullet” for High Photothermal Conversion in Dual-Mode Lateral Flow Immunoassay

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

Salmonella enterica serovar typhimurium (S. typhimurium), a prevalent foodborne bacterium, necessitates creating sensitive and rapid detection methods for food safety, with lateral flow immunoassays (LFIAs) using nanomaterials as signal tracers being particularly effective. Enhancements in performance sensitivity are not restricted to the material alone, we propose an "integrated stacked" concept, which combines amorphous active sites, hollow morphology enhanced reflection, symmetric structure strong absorption resonance. This approach leads significant photothermal enhancement (η = 60.66%) is supported by finite element analysis (|E|max2 3100). A "nanobullet" (RuTe2) was created, featuring vivid colorimetric enhancing range, large specific surface area (≈6:1) improved antibody binding, excellent properties facilitating dual-mode transduction. After 5 min of limits RuTe2-LFIA S. after 12 were 2398.83 cfu mL–1 (colorimetric) 977.23 (photothermal), 36.14 88.72 times lower than values AuNPs-LFIA (86696.19 mL–1). The superior suggests potential advancements materials point-of-care testing.

Язык: Английский

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"Molecular Velcro": Design of coupled AuNPs with Streptavidin-Biotin Immobilized Nanobody in Lateral Flow Immunoassay for sensitive Salmonella typhimurium Detection

Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137604 - 137604

Опубликована: Март 1, 2025

Язык: Английский

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Ultrasensitive point-of-care testing of salmonella in lettuce and milk samples using a recombinase polymerase amplification-based colorimetric/fluorescent dual-readout lateral flow assay

Food Chemistry, Год журнала: 2025, Номер unknown, С. 144058 - 144058

Опубликована: Март 1, 2025

Язык: Английский

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Metal and metal oxide nanoparticle-assisted molecular assays for the detection of Salmonella

Discover Nano, Год журнала: 2025, Номер 20(1)

Опубликована: Апрель 2, 2025

This paper provides a comprehensive overview of the diverse applications and innovations nanoparticles in detection Salmonella. It encompasses range novel methods, including efficient enrichment, nucleic acid extraction, immunoassays, tests, biosensors, emerging strategies with potential for future applications. The surface modification specific antibodies or ligands enables to achieve highly selective capture Salmonella, while optimizing extraction process improving efficiency. employment immunological tests markedly enhances specificity sensitivity reaction, thereby determination results. Moreover, distinctive physicochemical properties enhance sensitivity, selectivity, stability facilitating rapid advancement bio-detection technologies. is particularly noteworthy that there has been significant application innovative research nanozymes molecular assays. progress not only resulted enhanced efficiency but also facilitated innovation improvement As nanotechnologies continue advance, use metal oxide Salmonella likely become more promising reliable strategy ensuring food safety public health.

Язык: Английский

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