A new sesquiterpene with a specialized [4,4] bicyclononane skeleton from the marine-derived fungus Metarhizium sp. P2100
Zhong-Lian Ma,
Zhi‐Pu Yu,
Ling Lv
и другие.
Tetrahedron,
Год журнала:
2025,
Номер
unknown, С. 134607 - 134607
Опубликована: Март 1, 2025
Язык: Английский
A New Highly Oxidized 5/5/6 Tricyclic Sesquiterpene Skeleton from the Marine-Derived Fungus Metarhizium Sp. P2100
Опубликована: Янв. 1, 2025
Язык: Английский
Separation strategies of terpenoids from microbial fermentation broths
Trends in Food Science & Technology,
Год журнала:
2025,
Номер
unknown, С. 105098 - 105098
Опубликована: Май 1, 2025
Язык: Английский
Identification and Characterization of a Novel Multifunctional Terpene Synthase from the Pathogenic Fungus Cochliobolus heterostrophus
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 22, 2025
Sesquiterpenes,
a
structurally
diverse
class
of
natural
products,
play
pivotal
roles
in
the
pharmaceutical,
fragrance,
and
flavor
industries.
In
this
study,
we
first
identified
functionally
characterized
multifunctional
sesquiterpene
synthase,
ChTPS6,
from
plant-pathogenic
fungus
Cochliobolus
heterostrophus.
When
incubated
with
FPP,
ChTPS6
primarily
catalyzed
formation
cubebol
as
main
product,
which
is
widely
used
sustained
cooling
refreshing
agent
food
industry.
Remarkably,
exhibited
substrate
promiscuity,
efficiently
utilizing
GPP
GGPP
alternative
substrates.
Moreover,
through
protein
modeling
site-directed
mutagenesis,
pinpointed
key
amino
acid
residues
essential
for
catalytic
activity
successfully
re-engineered
enzyme
to
alter
its
product
specificity.
These
findings
provide
novel
insights
into
mechanism
functional
versatility
while
broadening
potential
applications
biosynthesis
valuable
sesquiterpenes.
Язык: Английский
Biological Properties of Sandalwood Oil and Microbial Synthesis of Its Major Sesquiterpenoids
Biomolecules,
Год журнала:
2024,
Номер
14(8), С. 971 - 971
Опубликована: Авг. 8, 2024
Sandalwood
essential
oil
is
extracted
from
the
heartwood
part
of
mature
sandalwood
and
known
for
its
pleasant
fragrance
exceptional
medicinal
activities,
including
antimicrobial,
antitumor,
anti-inflammatory
properties.
The
(Z)-α-santalol
(Z)-β-santalol
are
most
vital
ingredients
contributing
to
oil's
bioactivities
unique
woody
odor
characteristics.
Metabolic
engineering
strategies
have
shown
promise
in
transforming
microorganisms
such
as
yeast
bacteria
into
effective
cell
factories
enhancing
production
sesquiterpenes
(santalene
santalol)
found
oil.
This
review
aims
summarize
sources
oil,
components/ingredients,
applications.
It
also
highlights
biosynthesis
santalene
santalol
various
metabolic
employed
reconstruct
enhance
pathways
heterologous
hosts.
Язык: Английский
Development of a green Komagataella phaffii cell factory for sustainable production of plant-derived sesquiterpene (–)-α-bisabolol
Synthetic and Systems Biotechnology,
Год журнала:
2024,
Номер
10(1), С. 120 - 126
Опубликована: Сен. 17, 2024
Язык: Английский
Molecular Insight into the Catalytic Mechanism of the Sesquiterpene Cyclase BcABA3
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 17, 2024
BcABA3
is
an
unusual
sesquiterpene
synthase
that
lacks
the
conserved
DDxxD
and
DTE/NSE
motifs.
Despite
this,
it
can
catalyze
conversion
of
farnesyl
diphosphate
to
2Z,4E-α-ionylideneethane.
We
used
structure
prediction,
multiscale
simulations,
site-directed
mutagenesis
experiments
investigate
its
catalytic
mechanism.
has
structural
similarity
typical
class
I
terpenoid
cyclases
in
active
site.
Based
on
simulation
results,
we
identified
two
discontinuous
glutamate
residues,
E124
E88,
which
compensate
for
absence
aspartate-rich
motif.
Quantum
chemical
calculations
show
adopts
a
direct
rotation
mechanism
allyl
cation
isomerization
rather
than
via
nerolidyl
diphosphate.
Then,
achieve
successive
proton
transfer
reaction,
difficult
by
intramolecular
rearrangement
protruding
outward
carbonyl
oxygen
A206.
This
reaction
then
directed
forward
relatively
stable
intermediates
containing
cation-conjugated
double-bond
structure.
also
proposed
as
receptor
final
deprotonation
couple
this
step
with
2Z,4E-α-ionylideneethane
release.
These
findings
provide
valuable
insight
into
mechanisms
aid
engineering,
will
facilitate
studies
abscisic
acid
biosynthesis.
Язык: Английский