An R2R3‐type MYB transcription factor, GmMYB77, negatively regulates isoflavone accumulation in soybean [Glycine max (L.) Merr.] DOI
Yitian Liu, Shengrui Zhang, Jing Li

и другие.

Plant Biotechnology Journal, Год журнала: 2024, Номер unknown

Опубликована: Дек. 8, 2024

Summary Soybean [ Glycine max (L.) Merr.] is an exceptionally rich in isoflavones, and these compounds attach to oestrogen receptors the human body, lessening risk of breast cancer effectively alleviating menopausal syndrome symptoms. Uncovering molecular mechanisms that regulate soybean isoflavone accumulation crucial for enhancing production compounds. In this study, we combined bulk segregant analysis sequencing (BSA‐seq) a genome‐wide association study (GWAS) discover novel R2R3‐MYB family gene, GmMYB77 , regulates soybean. Using hairy root transient expression system, verified inhibits accumulation. Furthermore, knocking out significantly increased total (TIF) content, particularly malonylglycitin, while its overexpression resulted notable decrease contents malonylglycitin TIF. We found can directly binds core sequence GGT suppresses key biosynthesis genes Isoflavone synthase 1 ( GmIFS1 ), 2 GmIFS2 Chalcone 7 GmCHS7 ) 8 GmCHS8 by using dual‐luciferase assays, electrophoretic mobility shift assays yeast one‐hybrid experiments. Natural variations promoter region affect expression, thereby regulating TIF contents. Hap‐P2, elite haplotype, plays pivotal role breeding substantially enhanced content. These findings enhance our understanding influencing content provide valuable genetic resource efforts future.

Язык: Английский

An R2R3‐type MYB transcription factor, GmMYB77, negatively regulates isoflavone accumulation in soybean [Glycine max (L.) Merr.] DOI
Yitian Liu, Shengrui Zhang, Jing Li

и другие.

Plant Biotechnology Journal, Год журнала: 2024, Номер unknown

Опубликована: Дек. 8, 2024

Summary Soybean [ Glycine max (L.) Merr.] is an exceptionally rich in isoflavones, and these compounds attach to oestrogen receptors the human body, lessening risk of breast cancer effectively alleviating menopausal syndrome symptoms. Uncovering molecular mechanisms that regulate soybean isoflavone accumulation crucial for enhancing production compounds. In this study, we combined bulk segregant analysis sequencing (BSA‐seq) a genome‐wide association study (GWAS) discover novel R2R3‐MYB family gene, GmMYB77 , regulates soybean. Using hairy root transient expression system, verified inhibits accumulation. Furthermore, knocking out significantly increased total (TIF) content, particularly malonylglycitin, while its overexpression resulted notable decrease contents malonylglycitin TIF. We found can directly binds core sequence GGT suppresses key biosynthesis genes Isoflavone synthase 1 ( GmIFS1 ), 2 GmIFS2 Chalcone 7 GmCHS7 ) 8 GmCHS8 by using dual‐luciferase assays, electrophoretic mobility shift assays yeast one‐hybrid experiments. Natural variations promoter region affect expression, thereby regulating TIF contents. Hap‐P2, elite haplotype, plays pivotal role breeding substantially enhanced content. These findings enhance our understanding influencing content provide valuable genetic resource efforts future.

Язык: Английский

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