bta-miR-484 Inhibits Bovine Intramuscular Adipogenesis by Regulating Mitotic Clonal Expansion via the MAP3K9/JNK/CCND1 Axis DOI
Xinhao Ma,

Dianqi Zhang,

Zhimei Yang

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 24, 2024

Intramuscular fat (IMF) content is a critical indicator of the beef nutritional value and flavor. In this study, we focused on bta-miR-484, microRNA that differentially expressed during adipogenic differentiation bovine intramuscular adipocytes negatively correlated with IMF across different cattle breeds. Our findings demonstrate bta-miR-484 inhibits without altering fatty acid composition adipocytes. miRNA pull-down dual-luciferase reporter assays confirmed MAP3K9 target gene bta-miR-484. Furthermore, suppresses JNK signaling pathway by targeting MAP3K9, leading to decreased CCND1 expression, which impedes mitotic clonal expansion (MCE) process adipocyte differentiation. summary, study uncovers novel mechanism regulates provides first exploration MCE These offer valuable theoretical insights into breeding molecular improvements.

Язык: Английский

Effects of Phosphatidylethanolamine on Intramuscular Fat Deposition and Key Gene Identification by Transcriptome Sequencing in Broiler Chickens DOI Creative Commons
Lu Liu,

Hong Cui,

Nan Dong

и другие.

Poultry Science, Год журнала: 2025, Номер unknown, С. 104914 - 104914

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0

Transcriptomic Analysis of Muscle Satellite Cell Regulation on Intramuscular Preadipocyte Differentiation in Tan Sheep DOI Open Access
Xiaochun Xu,

Cong Zhan,

Jun-feng Qiao

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3414 - 3414

Опубликована: Апрель 5, 2025

Intramuscular fat (IMF) content is a key factor influencing meat properties including tenderness, flavor, and marbling. However, the complex molecular mechanisms regulating IMF deposition, especially interactions between intramuscular preadipocytes (IMAdCs) skeletal muscle satellite cells (SMSCs), remain unclear. In this study, direct co-culture system of sheep IMAdCs SMSCs was used to elucidate their intercellular interactions. RNA sequencing bioinformatics analyses were performed under monoculture conditions for later stages differentiation. The obtained results showed that significantly inhibited adipogenic capacity IMAdCs. This reflected in markedly altered gene expression observations lipid droplets our studies, i.e., PPARG, ACOX2, PIK3R1, FABP5, FYN, ALDOC, PFKM, PFKL, HADH, HADHB genes down-regulated co-cultured association with inhibition whereas ACSL3, ACSL4, ATF3, EGR1, IGF1R within upregulated associated promotion metabolism. addition, GO, KEGG, ligand-receptor pairing further elucidated communication. These findings emphasize regulatory role on preadipocyte differentiation metabolism, providing theoretical framework targeted strategies improve quality.

Язык: Английский

Процитировано

0

Integrated Multi-Tissue Lipidomics and Transcriptomics Reveal Differences in Lipid Composition Between Mashen and Duroc × (Landrace × Yorkshire) Pigs DOI Creative Commons
Mingyue Shi, Wenxia Li, Shuai Yang

и другие.

Animals, Год журнала: 2025, Номер 15(9), С. 1280 - 1280

Опубликована: Апрель 30, 2025

Chinese native pig breeds exhibit unique advantages over Western breeds, but the specific lipid metabolism mechanisms remain unclear. The phenotypic characteristics of Mashen (MS) pigs and Duroc × (Landrace Yorkshire) (DLY) are studied. results show that MS higher intramuscular fat (IMF) content. area adipocytes is significantly greater than in DLY (p < 0.01). Lipidomics analysis reveals distinct profiles upper layer backfat (ULB), leaf lard (LL), omentum (GOM), IMF, with showing polyunsaturated fatty acids (PUFAs) ULB, LL, GOM. Key differential lipids identified two include following triglycerides (TGs) phosphatidylcholines (PC): TG(16:1_18:1_18:3), TG(18:1_18:2_18:3), TG(18:3_18:2_18:2), PC(18:0_18:1), PC(18:0_18:2). Weighted gene co-expression network (WGCNA) molecules associated serum biochemical indices. Transcriptomics highlights 1944 differentially expressed genes between MS-ULB DLY-ULB. Notably, multiple from cytochrome P450 family (CYP2E1, CYP4A24, CYP2J2), along PLA2G2D, PLA2G4A, PCs, arachidonic linoleic acids. PLA2G2D PLA2G4A also involved α-linolenic This comprehensive provides essential information for breeding strategies meat quality improvement.

Язык: Английский

Процитировано

0

bta-miR-484 Inhibits Bovine Intramuscular Adipogenesis by Regulating Mitotic Clonal Expansion via the MAP3K9/JNK/CCND1 Axis DOI
Xinhao Ma,

Dianqi Zhang,

Zhimei Yang

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 24, 2024

Intramuscular fat (IMF) content is a critical indicator of the beef nutritional value and flavor. In this study, we focused on bta-miR-484, microRNA that differentially expressed during adipogenic differentiation bovine intramuscular adipocytes negatively correlated with IMF across different cattle breeds. Our findings demonstrate bta-miR-484 inhibits without altering fatty acid composition adipocytes. miRNA pull-down dual-luciferase reporter assays confirmed MAP3K9 target gene bta-miR-484. Furthermore, suppresses JNK signaling pathway by targeting MAP3K9, leading to decreased CCND1 expression, which impedes mitotic clonal expansion (MCE) process adipocyte differentiation. summary, study uncovers novel mechanism regulates provides first exploration MCE These offer valuable theoretical insights into breeding molecular improvements.

Язык: Английский

Процитировано

1