Effects of Phosphatidylethanolamine on Intramuscular Fat Deposition and Key Gene Identification by Transcriptome Sequencing in Broiler Chickens
Poultry Science,
Год журнала:
2025,
Номер
unknown, С. 104914 - 104914
Опубликована: Фев. 1, 2025
Язык: Английский
Transcriptomic Analysis of Muscle Satellite Cell Regulation on Intramuscular Preadipocyte Differentiation in Tan Sheep
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(7), С. 3414 - 3414
Опубликована: Апрель 5, 2025
Intramuscular
fat
(IMF)
content
is
a
key
factor
influencing
meat
properties
including
tenderness,
flavor,
and
marbling.
However,
the
complex
molecular
mechanisms
regulating
IMF
deposition,
especially
interactions
between
intramuscular
preadipocytes
(IMAdCs)
skeletal
muscle
satellite
cells
(SMSCs),
remain
unclear.
In
this
study,
direct
co-culture
system
of
sheep
IMAdCs
SMSCs
was
used
to
elucidate
their
intercellular
interactions.
RNA
sequencing
bioinformatics
analyses
were
performed
under
monoculture
conditions
for
later
stages
differentiation.
The
obtained
results
showed
that
significantly
inhibited
adipogenic
capacity
IMAdCs.
This
reflected
in
markedly
altered
gene
expression
observations
lipid
droplets
our
studies,
i.e.,
PPARG,
ACOX2,
PIK3R1,
FABP5,
FYN,
ALDOC,
PFKM,
PFKL,
HADH,
HADHB
genes
down-regulated
co-cultured
association
with
inhibition
whereas
ACSL3,
ACSL4,
ATF3,
EGR1,
IGF1R
within
upregulated
associated
promotion
metabolism.
addition,
GO,
KEGG,
ligand-receptor
pairing
further
elucidated
communication.
These
findings
emphasize
regulatory
role
on
preadipocyte
differentiation
metabolism,
providing
theoretical
framework
targeted
strategies
improve
quality.
Язык: Английский
Integrated Multi-Tissue Lipidomics and Transcriptomics Reveal Differences in Lipid Composition Between Mashen and Duroc × (Landrace × Yorkshire) Pigs
Animals,
Год журнала:
2025,
Номер
15(9), С. 1280 - 1280
Опубликована: Апрель 30, 2025
Chinese
native
pig
breeds
exhibit
unique
advantages
over
Western
breeds,
but
the
specific
lipid
metabolism
mechanisms
remain
unclear.
The
phenotypic
characteristics
of
Mashen
(MS)
pigs
and
Duroc
×
(Landrace
Yorkshire)
(DLY)
are
studied.
results
show
that
MS
higher
intramuscular
fat
(IMF)
content.
area
adipocytes
is
significantly
greater
than
in
DLY
(p
<
0.01).
Lipidomics
analysis
reveals
distinct
profiles
upper
layer
backfat
(ULB),
leaf
lard
(LL),
omentum
(GOM),
IMF,
with
showing
polyunsaturated
fatty
acids
(PUFAs)
ULB,
LL,
GOM.
Key
differential
lipids
identified
two
include
following
triglycerides
(TGs)
phosphatidylcholines
(PC):
TG(16:1_18:1_18:3),
TG(18:1_18:2_18:3),
TG(18:3_18:2_18:2),
PC(18:0_18:1),
PC(18:0_18:2).
Weighted
gene
co-expression
network
(WGCNA)
molecules
associated
serum
biochemical
indices.
Transcriptomics
highlights
1944
differentially
expressed
genes
between
MS-ULB
DLY-ULB.
Notably,
multiple
from
cytochrome
P450
family
(CYP2E1,
CYP4A24,
CYP2J2),
along
PLA2G2D,
PLA2G4A,
PCs,
arachidonic
linoleic
acids.
PLA2G2D
PLA2G4A
also
involved
α-linolenic
This
comprehensive
provides
essential
information
for
breeding
strategies
meat
quality
improvement.
Язык: Английский
bta-miR-484 Inhibits Bovine Intramuscular Adipogenesis by Regulating Mitotic Clonal Expansion via the MAP3K9/JNK/CCND1 Axis
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 24, 2024
Intramuscular
fat
(IMF)
content
is
a
critical
indicator
of
the
beef
nutritional
value
and
flavor.
In
this
study,
we
focused
on
bta-miR-484,
microRNA
that
differentially
expressed
during
adipogenic
differentiation
bovine
intramuscular
adipocytes
negatively
correlated
with
IMF
across
different
cattle
breeds.
Our
findings
demonstrate
bta-miR-484
inhibits
without
altering
fatty
acid
composition
adipocytes.
miRNA
pull-down
dual-luciferase
reporter
assays
confirmed
MAP3K9
target
gene
bta-miR-484.
Furthermore,
suppresses
JNK
signaling
pathway
by
targeting
MAP3K9,
leading
to
decreased
CCND1
expression,
which
impedes
mitotic
clonal
expansion
(MCE)
process
adipocyte
differentiation.
summary,
study
uncovers
novel
mechanism
regulates
provides
first
exploration
MCE
These
offer
valuable
theoretical
insights
into
breeding
molecular
improvements.
Язык: Английский