Human milk microbiota and oligosaccharides in colostrum and mature milk: comparison and correlation DOI Creative Commons

Hongda Ge,

Wenxiu Zhu,

Jing Zhang

и другие.

Frontiers in Nutrition, Год журнала: 2024, Номер 11

Опубликована: Дек. 12, 2024

Background The interaction between the human breast milk microbiota and oligosaccharides (HMOs) plays a crucial role in healthy growth development of infants. We aimed to clarify link HMOs at two stages lactation. Methods 20 colostrum samples (C group, 1–5 days postpartum) mature (S 42 collected from postpartum mothers were analyzed using 16S rRNA gene high-throughput sequencing high-performance liquid chromatography–tandem mass spectrometry. Result total average HMO content was significantly higher C group than S (6.76 ± 1.40 g/L vs. 10.27 2.00 g/L, p < 0.05). Among HMOs, values 2′-fucosyllactose (2′-FL, 1.64 1.54 3.03 1.79 g/L), 3′-sialyllactose (3′-SL, 0.10 0.02 0.21 0.06), 6′-SL (0.22 0.09 0.33 0.11 lacto-N-triaose 2 (LNT2, 0.03 0.01 0.16 0.08 g/L) lower ( 0.05), while that 3′-FL (1.35 1.00 0.41 0.43 diversity structure groups also different Comparative analysis microbial communities revealed Proteobacteria Firmicutes most abundant phyla, both groups, with keystone species Serratia , Streptococcus Staphylococcus ) closely interacting including 3′-SL, 6′-SL, LNT2. In PICRUSt2 functional prediction analysis, exhibited significant reduction expression genes involved several infectious disease pathways. Discussion Our findings support recognition as synbiotic comprising beneficial bacteria prebiotic HMOs.

Язык: Английский

Highly Efficient In Vivo Production of Sialyllacto-N-tetraose C via Screening of Beneficial β1,4-galactosyltransferase and α2,6-sialyltransferase DOI
Yuanlin Liu,

Qian Lin,

Mian Sheng

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер 73(9), С. 5376 - 5384

Опубликована: Фев. 18, 2025

Biological production of human milk oligosaccharides (HMOs) using metabolically engineered strains is a research hotspot in food biotechnology, but less effort has been made on the biological sialylated complex HMOs. Sialyllacto-N-tetraose c only monosialylated HMO top 15 In this study, metabolic pathway LST was constructed Escherichia coli BL21(DE3) by introducing three sequential glycosyltransferases: β1,3-N-acetylglucosaminyltransferase, β1,4-galactosyltransferase, and α2,6-sialyltransferase. The cytidine 5′-monophospho (CMP)-N-acetylneuraminic acid (Neu5Ac) enhanced to improve production. β1,4-galactosyltransferase from Helicobacter pylori J99 (HpGalT) α2,6-sialyltransferase Vespertiliibacter pulmonis (ED6ST) were screened as pair key glycosyltransferases for enhancing final strain could produce 1.718 9.745 g/L shake-flask fed-batch cultivation, respectively, indicating feasibility efficient biosynthesis

Язык: Английский

Процитировано

0
Efficient Biosynthesis of Sialyllacto-N-tetraose a by a Metabolically Engineered Escherichia coli BL21(DE3) Strain DOI

Mian Sheng,

Yuanlin Liu, Yingying Zhu

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 4, 2025

Recently, the construction of metabolically engineered strains for microbial synthesis human milk oligosaccharides (HMOs) has attracted increasing attention. However, fewer efforts were made in vivo biosynthesis complex HMOs, especially sialylated HMOs. In this study, we Escherichia coli BL21(DE3) to efficiently produce sialyllacto-N-tetraose a (LST-a) efficiently. Three sequential glycosylation steps introduced construct LST-a pathway, catalyzed by β1,3-N-acetylglucosaminylation, β1,3-galactosylation, and α2,3-sialylation. Pathway genes cytidine 5'-monophospho (CMP)-N-acetylneuraminic acid (Neu5Ac) support sialylation donor supply. Production was improved deleting competitive CMP-Neu5Ac synthesis, screening more efficient α2,3-sialyltransferase, combinatorial optimization pathway gene expression. finally produced with titer 1.235 4.85 g/L shake-flask fed-batch cultivation, respectively, demonstrating feasibility production

Язык: Английский

Процитировано

0
Regulation of Metabolic Pathways to Enhance Difucosyllactose Biosynthesis in Escherichia coli DOI

Shanquan Liang,

Qi Quan,

Dan Liu

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Дек. 19, 2024

Difucosyllactose (DFL), an important kind of fucosylated human milk oligosaccharides (HMOs), has garnered considerable attention due to its excellent physiological activities in infants. Previously, we achieved de novo biosynthesis DFL; however, substantial residual intermediates fucosyllactoses (FL) were detected. In this study, DFL was optimized, and FL reduced by regulating metabolic pathways. Different plasmid combinations used regulate gene expression, achieving optimal flux balance between 2′-FL DFL. The expression level key enzyme α-1,3-fucosyltransferase (α-1,3-FT, FucTa) then enhanced increasing copy number integrating fucTa into the chromosome. Exocytosis deleting sugar efflux transporter setA gene, thereby minimizing FL. Finally, strain BSF41 produced 55.3 g/L with only 2.59 a 5 L fermentor, representing highest reported titer date. This study provides foundation for advancing HMOs.

Язык: Английский

Процитировано

1
Human milk microbiota and oligosaccharides in colostrum and mature milk: comparison and correlation DOI Creative Commons

Hongda Ge,

Wenxiu Zhu,

Jing Zhang

и другие.

Frontiers in Nutrition, Год журнала: 2024, Номер 11

Опубликована: Дек. 12, 2024

Background The interaction between the human breast milk microbiota and oligosaccharides (HMOs) plays a crucial role in healthy growth development of infants. We aimed to clarify link HMOs at two stages lactation. Methods 20 colostrum samples (C group, 1–5 days postpartum) mature (S 42 collected from postpartum mothers were analyzed using 16S rRNA gene high-throughput sequencing high-performance liquid chromatography–tandem mass spectrometry. Result total average HMO content was significantly higher C group than S (6.76 ± 1.40 g/L vs. 10.27 2.00 g/L, p < 0.05). Among HMOs, values 2′-fucosyllactose (2′-FL, 1.64 1.54 3.03 1.79 g/L), 3′-sialyllactose (3′-SL, 0.10 0.02 0.21 0.06), 6′-SL (0.22 0.09 0.33 0.11 lacto-N-triaose 2 (LNT2, 0.03 0.01 0.16 0.08 g/L) lower ( 0.05), while that 3′-FL (1.35 1.00 0.41 0.43 diversity structure groups also different Comparative analysis microbial communities revealed Proteobacteria Firmicutes most abundant phyla, both groups, with keystone species Serratia , Streptococcus Staphylococcus ) closely interacting including 3′-SL, 6′-SL, LNT2. In PICRUSt2 functional prediction analysis, exhibited significant reduction expression genes involved several infectious disease pathways. Discussion Our findings support recognition as synbiotic comprising beneficial bacteria prebiotic HMOs.

Язык: Английский

Процитировано

0