Nanogap-Assisted SERS/PCR Biosensor Coupled Machine Learning for the Direct Sensing of Staphylococcus aureus in Food
Journal of Agricultural and Food Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 2, 2025
Staphylococcus
aureus
(S.
aureus)
is
the
primary
risk
factor
in
food
safety.
Herein,
a
nanogap-assisted
surface-enhanced
Raman
scattering/polymerase
chain
reaction
(SERS/PCR)
biosensor
coupled
with
machine-learning
tool
was
developed
for
direct
and
specific
sensing
of
S.
milk.
The
nuc
gene
(nuc
T)
from
initially
amplified
through
PCR
subsequently
captured
via
nanogap
effect
I–
Mg2+-mediated
bimetallic
gold
silver
nanoflowers
(Au/Ag
FL@I–-Mg2+).
These
nanogaps
generate
hotspots
signal
amplification
enclosed
T.
Subsequently,
tools
were
used
to
comparatively
analyze
collected
SERS
signals.
bootstrapping
soft
shrinkage-partial
least-squares
method
exhibited
superior
performance
(root
mean-square
error
prediction:
0.437,
prediction
set
correlation
coefficient:
0.967).
This
study
demonstrated
novel
label-free
strategy
specifically
detecting
aureus.
could
be
advanced
serve
as
platform
application
other
types
foodborne
pathogenic
bacteria
by
engineering
suitable
primer.
Язык: Английский
Magnetic field facilitated-colorimetric aptasensor for selective and sensitive detection of Staphylococcus aureus using aptamer-labeled Zn/Co ICPs nanoenzyme
Siwei Zhou,
Peipei Qiu,
Ruihua Feng
и другие.
Talanta,
Год журнала:
2025,
Номер
unknown, С. 127633 - 127633
Опубликована: Янв. 1, 2025
Язык: Английский
Autocrine peptides inhibited the formation of VBNC state of Staphylococcus aureus
Microbiological Research,
Год журнала:
2025,
Номер
294, С. 128103 - 128103
Опубликована: Фев. 14, 2025
Язык: Английский
Preparation of Carbon Dots with Peroxidase-like Activity and Their Application in Staphylococcus aureus Detection and Antimicrobial Susceptibility Test
Langmuir,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 3, 2025
Carbon
dot
(CD)
nanozymes
with
excellent
biocompatibility,
optical
properties,
and
catalytic
activity
show
great
promise
for
microbial
detection
drug
sensitivity
testing.
This
study
reports
the
synthesis
of
metal-doped
green-emitting
CDs
good
peroxidase-like
activity,
which
were
synthesized
via
a
one-step
hydrothermal
route
using
thiourea,
N-[3-(trimethoxysilyl)propyl]ethylenediamine,
catechol
as
starting
materials
FeCl3
doping
agent.
In
presence
H2O2,
catalyze
oxidation
3,3',5,5'-tetramethylbenzidine
(TMB),
producing
blue
product;
however,
in
bacteria
bacterial
catalase
enzyme
decomposes
H2O2
inhibits
CDs,
preventing
color
change.
The
neutralizes
prevents
from
color-changing
reaction
TMB.
Based
on
CDs-TMB-H2O2
cascade
system
bioenzymes
nanozymes,
we
developed
rapid,
sensitive,
direct
colorimetric
method
Staphylococcus
aureus
(S.
aureus)
limit
2
×
103
CFU/mL
linear
range
103-2
106
CFU/mL.
visual
was
successfully
applied
to
S.
food
samples.
Antibiotics
have
different
effects
proliferation
sensitive
resistant
strains,
leading
levels
hydrolysis
solution
resulting
varying
intensities
color;
therefore,
simple
antibiotic
susceptibility
test.
applications
CD
provide
powerful
tool
detecting
pathogenic
food,
clinical,
environmental
samples
infections.
Язык: Английский
Emerging Combination of Hydrogel and Electrochemical Biosensors
Small,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 16, 2024
Abstract
Electrochemical
sensors
are
among
the
most
promising
technologies
for
biomarker
research,
with
outstanding
sensitivity,
selectivity,
and
rapid
response
capabilities
that
make
them
important
in
medical
diagnostics
prognosis.
Recently,
hydrogels
have
gained
attention
domain
of
electrochemical
biosensors
because
their
superior
biocompatibility,
excellent
adhesion,
ability
to
form
conformal
contact
diverse
surfaces.
These
features
provide
distinct
advantages,
particularly
advancement
wearable
biosensors.
This
review
examines
contemporary
utilization
sensing,
explores
strategies
optimization
prospective
development
trajectories,
highlights
distinctive
advantages.
The
objective
is
an
exhaustive
overview
foundational
principles
sensing
systems,
analyze
compatibility
hydrogel
properties
methodologies,
propose
potential
healthcare
applications
further
illustrate
applicability.
Despite
significant
advances
hydrogel‐based
biosensors,
challenges
persist,
such
as
improving
material
fatigue
resistance,
interfacial
maintaining
balanced
water
content
across
various
environments.
Overall,
immense
flexible
exciting
opportunities.
However,
resolving
current
obstacles
will
necessitate
additional
research
efforts.
Язык: Английский
Rapid and sensitive detection of methicillin-resistant Staphylococcus aureus through the RPA-PfAgo system
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Авг. 21, 2024
Introduction
Both
the
incidence
and
mortality
rates
associated
with
methicillin-resistant
Staphylococcus
aureus
(MRSA)
have
progressively
increased
worldwide.
A
nucleic
acid
testing
system
was
developed
in
response,
enabling
swift
precise
detection
of
(
S.
)
its
MRSA
infection
status.
This
facilitates
improved
prevention
control
infections.
Methods
In
this
work,
we
introduce
a
novel
assay
platform
by
integrating
Pyrococcus
furiosus
Argonaute
Pf
Ago)
recombinase
polymerase
amplification
(RPA),
which
designed
for
simultaneous
nuc
mecA
genes
MRSA.
Results
innovative
approach
enables
visual
within
55
mins,
boasting
limit
10
2
copies/μL.
Characterized
high
specificity,
accurately
identifies
infections
without
cross-reactivity
to
other
clinical
pathogens,
highlighting
unique
capability
diagnostics
amidst
bacterial
diversity.
Validation
method
performed
on
40
isolates,
demonstrating
95.0%
accuracy
rate
comparison
established
Vitek2-COMPACT
system.
Discussion
The
RPA-
Ago
has
emerged
as
superior
diagnostic
tool,
offering
enhanced
sensitivity,
identification
efficacy
detection.
Our
findings
underscore
potential
significantly
improve
diagnosis
management
infection.
Язык: Английский