E3 Ubiquitin Ligase OsRFI2 Regulates Salinity Tolerance by Targeting Ascorbate Peroxidase OsAPX8 for its Degradation in Rice

Rice, Год журнала: 2025, Номер 18(1)

Опубликована: Март 10, 2025

Salinity is a major abiotic stress that adversely affects rice growth and production. However, the detailed regulatory mechanisms of salt response in remain largely unexplored. In this study, we established RING-type E3 ubiquitin ligase OsRFI2 plays negative role tolerance rice. Knockout mutants (Osrfi2) exhibited high tolerance, whereas OsRFI2-overexpressed transgenic lines (OE-OsRFI2) were more sensitive to stress. has activity interacts with ascorbate peroxidase OsAPX8 chloroplast, catalyzes its ubiquitination degradation through 26 S proteasome pathway. The Osapx8 mutants, like OE-OsRFI2 lines, showed sensitivity concentrations, accumulating greater amounts MDA, H2O2 O2−, which lead compromised cell permeability ROS accumulation. Thus, OsRFI2-OsAPX8 module adds novel clues for better understanding mechanism

Язык: Английский

New nano-ferro-silicon biochar promotes plant growth and grain yield under arsenic stress in rice

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Май 2, 2025

Arsenic (As) is a ubiquitous and toxic metalloid in nature, posing significant risks to living organisms. Developing sustainable strategies mitigate As stress reduce accumulation rice critical for ensuring food safety contaminated regions. Herein, we synthesized new nano-ferro-silicon biochar (NNFB) composed of biochar, γ-Fe 2 O 3 , SiO which effectively adsorbed from aqueous solutions soil. NNFB alleviated toxicity by promoting seeding root growth at the seed germination stages. Under 40 μM As(III) treatment, application 0.25% 0.5% regulated reactive oxygen species (ROS) balance reducing H enhancing peroxidase (POD) activity leaves. Additionally, reduced uptake regulating expression transport genes OsABCC1 OsLsi1 OsLsi2 stage. In pot experiments with mg/kg As(III)-contaminated soil, significantly improved aboveground biomass, tiller number, effective count. Notably, number per plant increased 6.93- 7.93-fold 1% treatments compared control. These findings demonstrate that efficiently adsorbs As, mitigates multiple stages, enhances productivity, offering promising solution As-contaminated agricultural systems.

Язык: Английский