Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 6, 2025

Selenoproteins (Sels) are a class of essential biomolecules that play critical roles in cellular homeostasis. SelO was identified as the preferential source selenium liver, implying its potential key regulatory factor hepatic pathophysiology. Bioinformatics analysis data from GEO sets revealed marked downregulation liver injury. However, function and mechanisms remain unclear. To address this, we investigated effect ablation on acute inflammation, focusing association with inflammation neutrophil extracellular traps (NETs) formation. Wild-type (WT) SelO-knockout mice were used to establish lipopolysaccharide (LPS) exposure model coculture (AML12 cells neutrophils) vitro. Our findings LPS stimulation significantly reduced expression WT mouse liver. deletion promoted Hmgb1 marker cytokines for chemokines, NETs generation, pyroptosis induced an imbalance redox Immunofluorescence, SYTOX staining, scanning electron microscopy confirmed silencing reactive oxygen species (ROS)-dependent Moreover, demonstrated excessive formation exacerbated SelO-ablation-induced inflammation. Importantly, significant involvement Hmgb1/ROS axis development absence SelO. results recruitment enhanced ROS-dependent by increasing levels, thereby aggravating LPS-induced This study not only uncovered crucial biological functions SelO, but also shed light implications inflammatory process.

Язык: Английский