Combinatorial Metabolic Engineering of Escherichia coli MG1655 Enables Efficient Biosynthesis of Vitamin B6 DOI
Xu Xue,

Z.W. Chen,

Haoran Sun

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 30, 2025

Pyridoxine (PN) is the most important commercial form of vitamin B6. Currently, PN chemically synthesized using toxic chemicals, causing environmental pollution. Microbial production B6 offers a more sustainable alternative, but its low productivity makes it difficult to meet commercialization demands. Here, metabolic engineering strategies were used efficiently improve production. Directed evolution 4-phosphoerythronate dehydrogenase led 4-fold increase in In addition, pdxK and pdxH genes then knocked out redirect flux from pyridoxine 5'-phosphate PN, involved glycerol-to-pentose phosphate metabolism overexpressed precursors for synthesis. Finally, recombinant strain produced 2120.1 ± 7.8 mg/L with 32.1 mg/L/h by batch fermentation. The reported here will be useful efficient biobased PN.

Язык: Английский

An industrial available platform for high-yield, plasmid-free recombinant protein production in E. coli based on advanced cSAT scheme DOI

Haifan Zhu,

Zhe-Yi Kuang,

Youyang Wang

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142208 - 142208

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Dual Antibiotic-Free Plasmid Systems Enable High-Efficiency l-Fucose Biosynthesis DOI
Jiawei Meng, Yingying Zhu, Zhen Lu

и другие.

ACS Synthetic Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 27, 2025

l-Fucose, a functional monosaccharide with significant commercial potential in the pharmaceutical, nutraceutical, and cosmetic industries, faces challenges microbial production due to antibiotic-dependent plasmid maintenance systems. This study presents dual antibiotic-free strategy engineered Escherichia coli BL21(DE3) achieve high-efficiency l-fucose biosynthesis. By integration of hok/sok toxin-antitoxin system cysC-based auxotrophic selection into two plasmids, genetic stability retention were ensured without antibiotics. Metabolic pathway optimization involved enhancing GDP-l-fucose supply via promoter replacements, genomic key enzymes (α1,2-fucosyltransferase α-l-fucosidase), blocking degradation. The strain demonstrated robust performance, producing 7.99 g/L shake-flask fermentation 61.91 fed-batch cultivation─both antibiotic-free. titer represents highest reported yield date, highlighting effectiveness combining toxin–antitoxin systems for sustainable, high-productivity manufacturing.

Язык: Английский

Процитировано

0
Combinatorial Metabolic Engineering of Escherichia coli MG1655 Enables Efficient Biosynthesis of Vitamin B6 DOI
Xu Xue,

Z.W. Chen,

Haoran Sun

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 30, 2025

Pyridoxine (PN) is the most important commercial form of vitamin B6. Currently, PN chemically synthesized using toxic chemicals, causing environmental pollution. Microbial production B6 offers a more sustainable alternative, but its low productivity makes it difficult to meet commercialization demands. Here, metabolic engineering strategies were used efficiently improve production. Directed evolution 4-phosphoerythronate dehydrogenase led 4-fold increase in In addition, pdxK and pdxH genes then knocked out redirect flux from pyridoxine 5'-phosphate PN, involved glycerol-to-pentose phosphate metabolism overexpressed precursors for synthesis. Finally, recombinant strain produced 2120.1 ± 7.8 mg/L with 32.1 mg/L/h by batch fermentation. The reported here will be useful efficient biobased PN.

Язык: Английский

Процитировано

0