Curcumin prevents dexamethasone-induced activation of the pseudorabies virus in rat pheochromocytoma cells through the miR-155-5p-Aak1-Numb/Notch2 signalling axis

Veterinary Research, Год журнала: 2025, Номер 56(1)

Опубликована: Апрель 21, 2025

Abstract Pseudorabies virus (PRV) causes neurological disorders and organ damage in diseased animals. After initial infection, PRV activity is gradually inhibited; however, stress stimulation increases the host’s glucocorticoid levels, which overcomes inhibition of activity. Curcumin (Cur) helps maintain inhibitory state Epstein-Barr virus, although further research needed to establish whether Cur can prevent activation triggered by hormones. In this study, we used PC-12 cells determine effects on activation. The were successfully infected with at a multiplicity infection 1 for 24 h, resulting Following incubation 0.5 µM dexamethasone (DEX) 4 was blocked. Further mechanistic analyses using dual-luciferase assay revealed that miR-155-5p directly targets regulates Aak1 its downstream signalling molecules, Numb Notch2, maintaining disrupting inhibition. Moreover, vitro experiments mimics inhibitors, combined overexpression interference, confirmed miR-155-5p-Aak1-Numb/Notch2 axis prevented DEX-induced disruption Cur. These findings provide novel regulatory target preventing stress-activated evidence potential use as modulator practical applications.

Язык: Английский

Development and characterisation of co-axial electrosprayed curcumin-loaded mesoporous silica and polymer composite coated microneedles

International Journal of Pharmaceutics, Год журнала: 2025, Номер unknown, С. 125722 - 125722

Опубликована: Май 1, 2025

Язык: Английский