Purification and Characterization of Cutinase from Fusarium Verticillioides and Its Application in Butyl Valerate Ester Synthesis

ChemistrySelect, Год журнала: 2025, Номер 10(9)

Опубликована: Март 1, 2025

Abstract Fusarium verticillioides isolated from soil was found to produce extracellular cutinase under optimized culture conditions. The enzyme purified using ion‐exchange and size‐exclusion chromatography, achieving a purification fold of 12.5 final yield 62%. molecular weight the determined be 25 kDa SDS‐PAGE. exhibited stability over temperature range 30–60 °C pH 6.0–9.0 retaining more than 85% its activity after 6 h incubation. It stable in presence various organic solvents (up 30% v/v) surfactants, whereas completely inhibited by serine protease inhibitor, that is, phenyl methane sulfonyl fluoride (PMSF), confirming an active residue catalytic site. Cutinase highly efficient catalyzing synthesis butyl valerate ester, 92% conditions: 0.25 M butanol, valeric acid, 30 mg enzyme, n ‐hexane as solvent at 50 for 8 h. ester purity were confirmed GCMS analysis. This study highlights potential robust biocatalyst with demonstrated efficiency high yields. Its application food industry sustainable alternative chemically synthesized esters is promising especially artificial flavoring aroma production. However, future studies are needed address scalability, long term storage stability, economic feasibility large‐scale production fully realize industrial potential.

Язык: Английский

Enhancing the Substrate Specificity of Nitrilase toward Aliphatic Nitriles Based on Substrate Channel Design

ACS Sustainable Chemistry & Engineering, Год журнала: 2025, Номер unknown

Опубликована: Март 29, 2025

Язык: Английский