Secretory and metabolic engineering of squalene in Yarrowia lipolytica

Bioresource Technology, Год журнала: 2025, Номер unknown, С. 132171 - 132171

Опубликована: Фев. 1, 2025

Язык: Английский

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Combinatorial metabolic engineering for overproduction of squalene in Saccharomyces cerevisiae

Systems Microbiology and Biomanufacturing, Год журнала: 2025, Номер unknown

Опубликована: Фев. 24, 2025

Язык: Английский

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Novel cell factory for the production of 24-epi-ergosterol, an un-natural semi-synthetic precursor for the production of brassinolide in Yarrowia lipolytica

World Journal of Microbiology and Biotechnology, Год журнала: 2025, Номер 41(3)

Опубликована: Март 1, 2025

Язык: Английский

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Construction of Saccharomyces cerevisiae Platform Strain for the Biosynthesis of Carotenoids and Apocarotenoids

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Carotenoids and apocarotenoids, natural compounds with vital biological functions, are now sustainably produced via microbial synthesis as an eco-friendly alternative to inefficient polluting traditional plant-based extraction methods. In their biosynthesis, β-carotene (BC) plays a crucial role it is the key intermediate from which different downstream derivatives formed. Here, we engineered high-producing Saccharomyces cerevisiae platform strain produce BC through combination of systematic metabolic engineering atmospheric room temperature plasma mutagenesis. The achieved production 2.09 g/L fed-batch fermentation in 5-L bioreactor, highest yield reported S. date. Using this strain, constructed zeaxanthin- β-ionone-producing strains by introducing enzyme genes. 39.09 mg/L zeaxanthin 31.87 β-ionone shake-flask cultures. established study provides higher starting point for producing diverse derivatives.

Язык: Английский

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Optimization of Squalene Production by Pseudozyma sp. P4-22

Molecules, Год журнала: 2025, Номер 30(7), С. 1646 - 1646

Опубликована: Апрель 7, 2025

Squalene is an important bioactive substance widely used in the food, pharmaceutical, and cosmetic industries. Microbial production of squalene has gained prominence recent years due to its sustainability, safety, environmental friendliness. In this study, a mutant strain, Pseudozyma sp. P4-22, with enhanced squalene-producing ability, was obtained through atmospheric room temperature plasma mutagenesis previously screened yeast SD301. The P4-22 strain demonstrated ability produce using various carbon nitrogen sources. We optimized culture conditions by employing cost-effective corn steep liquor as source, optimal pH sea salt concentration medium were determined be 5.5 5 g/L, respectively. Under cultivation conditions, biomass reached 64.42 g/L 2.06 respectively, L fed-batch fermentation. This study highlights potential promising for commercial-scale squalene.

Язык: Английский

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Reprogramming Schizochytrium cell factory for High-Yield production of astaxanthin via mutagenesis and metabolic engineering

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 163556 - 163556

Опубликована: Май 1, 2025

Язык: Английский

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