Apigenin-Mediated ESCRT-III Activation and Mitophagy Alleviate LPS-Induced Necroptosis in Renal Cells DOI

Jiakang Guo,

Jin-Ping Hu,

Meitong Liu

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 10, 2025

Apigenin (API) is a flavonoid widely distributed in vegetables and fruits that exhibits numerous biological functions. Lipopolysaccharide (LPS), key component of the outer membrane Gram-negative bacteria, can cause kidney injury when released into bloodstream. Necroptosis form programmed cell death characterized by rupture membranes. Excessive occurrence necroptosis lead to substantial damage cells tissues. In study, we discovered API could mitigate LPS-induced mice alleviate Normal Rat Kidney-52E (NRK-52E) targeting mitochondrial reactive oxygen species (mtROS)-RIPK3-MLKL pathway. Further mechanistic studies revealed potentially activate endosomal sorting complexes required for transport-III (ESCRT-III), activated ESCRT-III repair caused necroptosis. Simultaneously, promote mitophagy, which facilitates timely removal damaged mitochondria reduces intracellular mtROS levels. conclusion, our results suggested alleviates renal activating ESCRT-III-dependent mitophagy. Our study provides new insights daily dietary intake LPS.

Язык: Английский

Glyphosate combined with TBBPA exposure decreased quality and flavor of common carp (Cyprinus carpio) involved inhibiting muscle growth and collagen synthesis DOI
Dong‐Xu Han,

Yanju Bi,

Tingting Yu

и другие.

Pesticide Biochemistry and Physiology, Год журнала: 2025, Номер 209, С. 106344 - 106344

Опубликована: Фев. 21, 2025

Язык: Английский

Процитировано

1

Liver injury in sepsis: manifestations, mechanisms and emerging therapeutic strategies DOI Creative Commons

Xinqi Xu,

Tingyu Yang, Jie An

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Март 28, 2025

Sepsis is defined as a condition related to infection that manifests with multiorgan dysfunction, representing life-threatening state. Consequently, severe complications frequently occur, liver injury being one of the most prevalent serious sepsis. Liver dysfunction during sepsis serves an independent predictor mortality. This review provides comprehensive overview current research on sepsis-induced (SILI), encompassing clinical manifestations, diagnostic criteria, pathogenesis and therapeutic strategies associated this condition. SILI may manifest hypoxic hepatitis due ischemia shock, cholestasis resulting from abnormal bile metabolism, or duct sclerosis. The pathophysiology involves intricate interactions among inflammatory response, oxidative stress, cell death. All these factors complicate treatment represent potential targets for intervention. Furthermore, addresses limitations inherent in conventional therapies currently employed managing emphasizes novel targeted aimed at addressing fundamental mechanisms underlying

Язык: Английский

Процитировано

0

Importance of Selenoprotein O in Regulating Hmgb1: A New Direction for Modulating ROS-Dependent NETs Formation to Aggravate the Progression of Acute Liver Inflammation DOI
Xia Yu, Kai Chen, Yidan Wang

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 6, 2025

Selenoproteins (Sels) are a class of essential biomolecules that play critical roles in cellular homeostasis. SelO was identified as the preferential source selenium liver, implying its potential key regulatory factor hepatic pathophysiology. Bioinformatics analysis data from GEO sets revealed marked downregulation liver injury. However, function and mechanisms remain unclear. To address this, we investigated effect ablation on acute inflammation, focusing association with inflammation neutrophil extracellular traps (NETs) formation. Wild-type (WT) SelO-knockout mice were used to establish lipopolysaccharide (LPS) exposure model coculture (AML12 cells neutrophils) vitro. Our findings LPS stimulation significantly reduced expression WT mouse liver. deletion promoted Hmgb1 marker cytokines for chemokines, NETs generation, pyroptosis induced an imbalance redox Immunofluorescence, SYTOX staining, scanning electron microscopy confirmed silencing reactive oxygen species (ROS)-dependent Moreover, demonstrated excessive formation exacerbated SelO-ablation-induced inflammation. Importantly, significant involvement Hmgb1/ROS axis development absence SelO. results recruitment enhanced ROS-dependent by increasing levels, thereby aggravating LPS-induced This study not only uncovered crucial biological functions SelO, but also shed light implications inflammatory process.

Язык: Английский

Процитировано

0

Apigenin-Mediated ESCRT-III Activation and Mitophagy Alleviate LPS-Induced Necroptosis in Renal Cells DOI

Jiakang Guo,

Jin-Ping Hu,

Meitong Liu

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 10, 2025

Apigenin (API) is a flavonoid widely distributed in vegetables and fruits that exhibits numerous biological functions. Lipopolysaccharide (LPS), key component of the outer membrane Gram-negative bacteria, can cause kidney injury when released into bloodstream. Necroptosis form programmed cell death characterized by rupture membranes. Excessive occurrence necroptosis lead to substantial damage cells tissues. In study, we discovered API could mitigate LPS-induced mice alleviate Normal Rat Kidney-52E (NRK-52E) targeting mitochondrial reactive oxygen species (mtROS)-RIPK3-MLKL pathway. Further mechanistic studies revealed potentially activate endosomal sorting complexes required for transport-III (ESCRT-III), activated ESCRT-III repair caused necroptosis. Simultaneously, promote mitophagy, which facilitates timely removal damaged mitochondria reduces intracellular mtROS levels. conclusion, our results suggested alleviates renal activating ESCRT-III-dependent mitophagy. Our study provides new insights daily dietary intake LPS.

Язык: Английский

Процитировано

0