The Impact of Human Milk Oligosaccharides on Antibiotic-Induced Microbial Dysbiosis and Gut Inflammation in Mice
Antibiotics,
Год журнала:
2025,
Номер
14(5), С. 488 - 488
Опубликована: Май 10, 2025
Background/Objectives:
Antibiotics
have
a
significant
impact
on
the
gut
microbiota,
and
we
hypothesized
that
human
milk
oligosaccharides
may
alleviate
antibiotic-induced
microbiota
dysbiosis.
Methods:
Six
groups
of
eight
mice
were
administered
drinking
water
with
or
without
ampicillin
for
one
week.
We
then
introduced
oligosaccharide
2'-fucosyllactose
(2'FL),
either
alone
in
combination
difucosyl-lactose
(DFL),
two
weeks
after
termination
treatment.
Results:
Ampicillin
reduced
diversity
abundance
specific
bacteria.
One
week
treatment,
2'FL
+
DFL
mixture
counteracted
ampicillin-induced
reduction
diversity,
although
this
effect
was
not
sustained.
Over
subsequent
weeks,
had
relative
abundances
Lactobacillus
spp.
Bacteroides
also
caecal
propionate
levels,
downregulated
gene
Gzmb
Granzyme
B,
upregulated
Reg3a
Regenerating
islet-derived
protein
3
alpha,
all
which
by
mixture.
minor
ileal
cytokine
levels.
The
showed
indicating
adaptive
innate
inflammation.
intake
growth
mice.
did
affect
intake,
but
slightly
body
weight.
Conclusions:
appears
to
hold
potential
counteracting
some
side
effects
Язык: Английский
High-Fat Diet-Induced Mild Obesity Alters the Activation of T Cells and Maintains Intestinal Homeostasis in Food Allergy Animal Model
Foods,
Год журнала:
2025,
Номер
14(11), С. 1852 - 1852
Опубликована: Май 23, 2025
A
close
association
between
obesity
and
food
allergies
has
been
described,
but
from
the
perspective
of
T
cell
differentiation,
controversial
findings
exist
regarding
impact
high-fat
diet
(HFD)
feeding
on
development
allergies.
Our
study
aimed
to
explore
relationship
HFD-induced
mild
allergy
in
female
BALB/c
mice.
Following
18
weeks
HFD
feeding,
experimental
animals
demonstrated
a
10.92%
body
weight
gain
accompanied
by
32.45%
elevation
total
cholesterol
levels
39.50%
increase
low-density
lipoprotein–cholesterol
levels.
Upon
allergen
challenge,
control
(COD)-fed
mice
developed
obvious
symptoms
hypothermia,
which
were
slightly
alleviated
HFD-fed
Flow
cytometry
revealed
that
splenocytes
exhibited
102.32%
CD4+T-bet+
(Th1)
populations
38.69%
CD4+RORγt+
(Th17)
compared
COD-fed
allergic
These
changes
corresponded
with
28.41%
reduction
Th2/Th1
ratio
56.67%
Th17/Treg
ratio.
Moreover,
real-time
quantitative
PCR
showed
had
higher
expressions
Foxp3,
Occludin1,
TJP1
intestine.
Therefore,
we
concluded
restored
balance,
reinforced
intestinal
barrier
function,
thereby
did
not
risk
Язык: Английский