Enthalpy-Driven Interaction between Bovine Serum Albumin and Biomass-Derived Low-Melting Mixture Solvents (LoMMSs) for Efficient and Green Purification of Protein

Biomacromolecules, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

Green separation of protein (e.g., bovine serum albumin (BSA)) by low-melting mixture solvents (LoMMSs) depends on the underlying mechanism between BSA and LoMMSs. Here, we for first time find that eco-friendly biomass-derived LoMMSs could be potentially used efficient green purification enthalpy-driven interactions. Biomass-derived possess merits high biocompatibility, degradability, abundance, low cost. A single high-affinity binding site via hydrogen bonding van der Waals forces is observed fluorescence thermodynamic analysis. Experimental results from circular dichroism infrared spectra demonstrate addition stabilizes secondary structure protein. This work provides a valuable indication design cost-effective

Язык: Английский

Rational design of hydrophobic type Ⅴ deep eutectic solvents as efficient CO2 absorbents

Chemical Engineering Science, Год журнала: 2025, Номер unknown, С. 121904 - 121904

Опубликована: Май 1, 2025

Язык: Английский