Functional characterization and site-directed mutagenesis of a novel UDP-glycosyltransferase from Panax japonicus var. major
Planta,
Год журнала:
2025,
Номер
261(3)
Опубликована: Янв. 31, 2025
Язык: Английский
Proteomic Analysis of Midgut of Silkworm Reared on Artificial Diet and Mulberry Leaves and Functional Study of Three UGT Genes
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(3), С. 1309 - 1309
Опубликована: Фев. 4, 2025
There
remains
a
significant
gap
in
production
performance
and
disease
resistance
between
silkworms
reared
on
artificial
diets
those
mulberry
leaves.
This
study
aims
to
identify
key
differential
proteins
through
proteomic
analysis
of
the
midgut
fed
compared
Utilizing
molecular
docking
technology,
three
anti-nutritional
factors
that
consistently
bind
UGT40B4,
UGT340C2,
UGT40A1
were
selected,
expression
these
UGT
genes
response
various
was
examined.
The
findings
indicate
variations
feed
significantly
influence
digestive,
metabolic,
immune-related
within
silkworm
midgut.
Notably,
levels
vary
across
different
organs
developmental
stages,
reflecting
their
respective
physiological
roles.
Furthermore,
effects
soybean
isoflavone,
tannic
acid,
arabinoxylan
growth
cocoon
quality
found
differ
when
substances
incorporated
into
semi-synthetic
feed.
research
is
anticipated
provide
valuable
insights
for
future
studies
role
formulation
silkworms.
Язык: Английский
Enhancing active ingredient biosynthesis in Chinese herbal medicine: biotechnological strategies and molecular mechanisms
PeerJ,
Год журнала:
2025,
Номер
13, С. e18914 - e18914
Опубликована: Фев. 10, 2025
Background
Chinese
herbal
medicine
(CHM)
is
a
fundamental
component
of
traditional
medical
practice,
offering
rich
source
natural
remedies
with
significant
therapeutic
potential.
However,
the
scarcity
active
ingredients
and
complex
extraction
procedures
present
substantial
challenges
to
their
widespread
clinical
application.
This
review
aims
address
this
gap
by
exploring
potential
modern
biotechnological
advancements
in
enhancing
biosynthesis
these
valuable
compounds.
Methodology
The
study
takes
comprehensive
approach,
delving
into
chemical
composition
CHM’s
elucidating
biosynthetic
pathways
molecular
regulatory
mechanisms.
Additionally,
it
surveys
recent
progress
methodologies
evaluates
engineering
strategies
aimed
at
synthetic
production.
multifaceted
analysis
forms
foundation
for
examining
role
biology
augmenting
ingredient
synthesis.
Results
Our
examination
provides
insights
intricate
governing
formation
ingredients,
as
well
networks
that
underlie
processes.
Furthermore,
highlights
techniques,
demonstrating
ability
streamline
enhance
isolation
Engineering
approaches
production,
including
metabolic
tools,
are
assessed
overcome
limitations
scale
up
Conclusions
By
integrating
from
biosynthesis,
regulation,
methodologies,
biology,
establishes
robust
theoretical
framework
production
ingredients.
proposed
practical
guidance
aim
facilitate
broader
utilization
while
promoting
sustainability
accessibility
within
invaluable
medicinal
heritage.
Язык: Английский
Anemarrhena asphodeloides Bunge: A review of its biological characteristics, steroidal saponin biosynthesis and their molecular mechanisms
Industrial Crops and Products,
Год журнала:
2025,
Номер
227, С. 120765 - 120765
Опубликована: Фев. 28, 2025
Язык: Английский
Phylogenetic Analysis and Expression Patterns of Triterpenoid Saponin Biosynthesis Genes in 19 Araliaceae Plants
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(7), С. 3439 - 3439
Опубликована: Апрель 7, 2025
The
Araliaceae
family
has
significant
economic
and
medicinal
value.
However,
the
phylogenetic
relationships
expression
patterns
of
key
genes
active
triterpenoid
substance
within
this
are
still
unclear.
In
study,
we
employed
comparative
transcriptomics
to
analyze
transcriptomes
19
species
from
11
genera
Araliaceae,
aiming
elucidate
evolutionary
history
in
ginsenoside
biosynthesis
pathway.
Our
results
divide
into
two
subfamilies:
Aralioideae
Hydrocotyloideae.
is
further
classified
three
groups:
Aralia-Panax
group,
Polyscias-Pseudopanax
Asian
Palmate
group.
PhyloNet
analysis
reveals
that
common
ancestor
Panax
ginseng,
quinquefolius,
japonicus
was
an
allopolyploid,
likely
resulting
hybridization
between
notoginseng
pseudoginseng.
Additionally,
all
underwent
pg-β
event,
which
may
be
critical
for
biosynthesis.
We
discovered
exhibit
distinct
enzyme
(β-AS,
DDS,
CYP450,
UGTs)
compared
other
species.
These
show
independent
lineages
gene
trees,
suggesting
unique
functional
adaptations
enable
efficiently
synthesize
ginsenosides.
This
study
provides
a
theoretical
foundation
conservation
utilization
germplasm
resources.
Язык: Английский
Immobilization of β-glucosidase and β-xylosidase on inorganic nanoparticles for glycosylated substances conversion
International Journal of Biological Macromolecules,
Год журнала:
2024,
Номер
292, С. 139173 - 139173
Опубликована: Дек. 26, 2024
Язык: Английский
Characterization of a Xylosyltransferase from Panax notoginseng Catalyzing Ginsenoside 2′-O Glycosylation in the Biosynthesis of Notoginsenosides
Journal of Natural Products,
Год журнала:
2024,
Номер
87(9), С. 2160 - 2169
Опубликована: Авг. 27, 2024
Notoginsenosides
are
important
bioactive
compounds
from
Язык: Английский
Insights into functional divergence, catalytic versatility and specificity of small molecule glycosyltransferases
International Journal of Biological Macromolecules,
Год журнала:
2024,
Номер
unknown, С. 138821 - 138821
Опубликована: Дек. 1, 2024
Язык: Английский
Recent trends in the elucidation of complex triterpene biosynthetic pathways in horticultural trees
Horticulture Research,
Год журнала:
2024,
Номер
12(1)
Опубликована: Окт. 6, 2024
Abstract
Triterpene
(C30
isoprene
compounds)
represents
the
most
structurally
diverse
class
of
natural
products
and
has
been
extensively
exploited
in
food,
medicine,
industrial
sectors.
Decades
research
on
medicinal
triterpene
biosynthetic
pathways
have
revealed
their
roles
stress
tolerance
shaping
microbiota.
However,
biological
function
mechanism
triterpenes
are
not
fully
identified.
Even
this
scientific
window
narrows
down
for
horticultural
trees.
The
lack
knowledge
a
scalable
production
system
limits
discovery
pathways.
Recent
synthetic
biology
several
important
that
define
address
many
societal
sustainability
challenges.
Here,
I
review
chemical
diversity
enzymes
involved
biosynthesis
This
also
outlines
integrated
Design-Build-Test-Learn
(DBTL)
pipelines
discovery,
characterization,
optimization
Further,
these
DBTL
components
share
fundamental
technical
difficulties,
highlighting
opportunities
interdisciplinary
collaboration
between
researchers
worldwide.
advancement
opens
up
unprecedented
bioengineering
compounds
toward
development
scaleup
processes.
Язык: Английский
Discovering a novel glycosyltransferase gene CmUGT1 enhances main metabolites production of Cordyceps militaris
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Окт. 22, 2024
Cordyceps
militaris
is
a
filamentous
fungus
used
for
both
medicinal
and
culinary
purposes.
It
exhibits
wide
range
of
pharmacological
activities
due
to
its
valuable
contents
cordycepin,
polysaccharides,
carotenoids,
terpenoids
other
metabolites.
However,
C.
strains
are
highly
susceptible
irreversible
degradation
in
agricultural
production,
which
often
manifested
as
prolonged
color
change
period
significant
decrease
the
production
secondary
UDP-glycosyltransferases
an
important
enzyme
family
that
participates
synthesis
by
performing
glycosylation
key
residues
enzymes
or
molecules.
few
studies
have
focused
on
effect
regulation
metabolite
.
Therefore,
this
study,
we
performed
transcriptome
analysis
across
four
different
developmental
stages
target
putative
glycosyltransferase
gene
CmUGT1,
plays
roles
production.
We
further
constructed
screened
CmUGT1
-overexpressing
strain
Agrobacterium
tumefaciens
-mediated
infestation
spores.
The
major
wild-type
was
determined
after
short-term
shake-flask
cultivation
mycelia.
results
showed
yields
carotenoids
polysaccharides
mycelia
were
3.8
3.4
times
greater
than
those
wild
type,
respectively
(
p
<
0.01).
levels
intracellular
extracellular
cordycepin
produced
overexpression
4.4
8.0
This
suggests
enhances
main
related
provides
guide
obtaining
excellent
recombinant
Язык: Английский