A high-throughput pipeline for design and selection of peptides targeting the SARS-Cov-2 Spike protein DOI Creative Commons
Monica Wolfe, Sean Webb, Yaroslav Chushak

и другие.

Scientific Reports, Год журнала: 2021, Номер 11(1)

Опубликована: Ноя. 5, 2021

Rapid design, screening, and characterization of biorecognition elements (BREs) is essential for the development diagnostic tests antiviral therapeutics needed to combat spread viruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To address this need, we developed a high-throughput pipeline combining in silico design peptide library specific SARS-CoV-2 spike (S) protein microarray screening identify binding sequences. Our optimized platform allowed simultaneous ~ 2.5 k peptides rapid identification sequences resulting selection four with nanomolar affinity S protein. Finally, demonstrated successful integration one top into an electrochemical sensor clinically relevant limit detection spiked saliva. results demonstrate utility novel BREs response pandemic, broader application future viral threats.

Язык: Английский

A review on structural, non-structural, and accessory proteins of SARS-CoV-2: Highlighting drug target sites DOI Open Access
Md. Jahirul Islam, Nafisa Nawal Islam, Md. Siddik Alom

и другие.

Immunobiology, Год журнала: 2022, Номер 228(1), С. 152302 - 152302

Опубликована: Ноя. 15, 2022

Язык: Английский

Процитировано

35
Antiviral Peptides (AVPs) of Marine Origin as Propitious Therapeutic Drug Candidates for the Treatment of Human Viruses DOI Creative Commons
Linda Sukmarini

Molecules, Год журнала: 2022, Номер 27(9), С. 2619 - 2619

Опубликована: Апрель 19, 2022

The marine environment presents a favorable avenue for potential therapeutic agents as reservoir of new bioactive natural products. Due to their numerous pharmacological effects, marine-derived products—particularly peptides—have gained considerable attention. These peptides have shown broad spectrum biological functions, such antimicrobial, antiviral, cytotoxic, immunomodulatory, and analgesic effects. emergence virus strains viral resistance leads continuing efforts develop more effective antiviral drugs. Interestingly, antimicrobial (AMPs) that possess properties are alternatively regarded (AVPs) demonstrate vast alternative peptide-based drug candidates available infection treatments. Hence, AVPs obtained from various organisms been evaluated. This brief review features recent updates 2011 2021. Moreover, the biosynthesis this class compounds possible mechanisms action also discussed. Selected possessing activities against important human viruses—such immunodeficiency viruses, herpes simplex influenza hepatitis C virus, coronaviruses—are highlighted herein.

Язык: Английский

Процитировано

32
Novel 2D layered g-C3N4 nanocomposite materials for sustainable wastewater treatment by catalytic degradation of toxic dye DOI Creative Commons

Most Munera Khatun,

Mohammad Raza Miah,

Chunjie Yan

и другие.

Optik, Год журнала: 2024, Номер 311, С. 171959 - 171959

Опубликована: Июль 11, 2024

The photocatalytic degradation technique is one of the suitable ways dealing with environmental pollutant decontamination. This study demonstrates ability graphitic carbon nitride (g-C3N4) nanocomposites containing tellurium (Te) and molybdenum disulfide (MoS2) to photocatalytically destroy organic cationic methylene blue (MB) dye, which primarily used in textile industry. Using alkali treatment approach, layered was created. MoS2 Te, several g-C3N4 were created using ultrasonic calcination techniques. After Te immobilized individually, composites underwent a methodical characterization process utilizing variety instruments, including XRD SEM. 2D nano-g-C3N4 substrate, photo-catalytic reaction MB observed, UV spectrophotometer determine absorbance dye. 0.2 % MoS2/g-C3N4 5 Te/g-C3N4, efficiency dye (10, 15, 20 mg/L) solution examined. In comparison other produced nanocomposites, Te/g-C3N4 composite shown have highest activity terms ability, about 100 dyes degraded less than 60 minutes. As concentration increased, steadily dropped, rate around 1.5 mg/L.

Язык: Английский

Процитировано

7
Computational screening of 645 antiviral peptides against the receptor-binding domain of the spike protein in SARS-CoV-2 DOI Open Access
Md Minhas Hossain Sakib, Aktiya Anjum Nishat, Mohammad Tarequl Islam

и другие.

Computers in Biology and Medicine, Год журнала: 2021, Номер 136, С. 104759 - 104759

Опубликована: Авг. 10, 2021

Язык: Английский

Процитировано

41
Bomidin: An Optimized Antimicrobial Peptide With Broad Antiviral Activity Against Enveloped Viruses DOI Creative Commons
Rongrong Liu, Ziyu Liu, Haoran Peng

и другие.

Frontiers in Immunology, Год журнала: 2022, Номер 13

Опубликована: Май 19, 2022

The rapid evolution of highly infectious pathogens is a major threat to global public health. In the front line defense against bacteria, fungi, and viruses, antimicrobial peptides (AMPs) are naturally produced by all living organisms offer new possibilities for next-generation antibiotic development. However, low yields difficulties in extraction purification AMPs have hindered their industry scientific research applications. To overcome these barriers, we enabled high expression bomidin, commercial recombinant AMP based upon bovine myeloid peptide-27. This novel AMP, which can be expressed Escherichia coli adding methionine bomidin sequence, bulk more biologically active than chemically synthesized AMPs. We verified function variety bacteria enveloped including severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), herpes simplex virus (HSV), dengue (DENV), chikungunya (CHIKV). Furthermore, on molecular modeling membrane lipids, elucidated possible mechanism disrupts bacterial viral membranes. Thus, obtained with an optimized, efficient heterologous system potential therapeutic application wide range life-threatening pathogens.

Язык: Английский

Процитировано

29
Synthesis and characterization of novel copper(ii) complexes as potential drug candidates against SARS-CoV-2 main protease DOI
Sunil Kumar, Mukesh Choudhary

New Journal of Chemistry, Год журнала: 2022, Номер 46(10), С. 4911 - 4926

Опубликована: Янв. 1, 2022

Two novel copper( ii ) Schiff base complexes, [Cu(L 1 2 ] (1) and )(CH 3 OH)(Cl)] (2) of [( Z )-(5-chloro-2-((3,5-dichloro-2-hydroxybenzylidene)amino)phenyl)(phenyl)methanone (L H) ( )-(2((5-bromo-2-hydroxybenzylidene)amino-5-chlorophenyl)(phenyl)methanone)(L H)], have been designed, synthesized characterized.

Язык: Английский

Процитировано

22
Leveraging the therapeutic, biological, and self-assembling potential of peptides for the treatment of viral infections DOI
Maya K. Monroe, Han Wang, Caleb F. Anderson

и другие.

Journal of Controlled Release, Год журнала: 2022, Номер 348, С. 1028 - 1049

Опубликована: Июль 6, 2022

Язык: Английский

Процитировано

20
Amaranth proteins and peptides: Biological properties and food uses DOI
Fan Zhu

Food Research International, Год журнала: 2022, Номер 164, С. 112405 - 112405

Опубликована: Дек. 29, 2022

Язык: Английский

Процитировано

20
In vitro and in vivo suppression of SARS‐CoV‐2 replication by a modified, short, cell‐penetrating peptide targeting the C‐terminal domain of the viral spike protein DOI

Dongbum Kim,

Jin-Soo Kim, Seungchan An

и другие.

Journal of Medical Virology, Год журнала: 2023, Номер 95(3)

Опубликована: Март 1, 2023

Abstract Peptides are promising therapeutic agents for COVID‐19 because of their specificity, easy synthesis, and ability to be fine‐tuned. We previously demonstrated that a cell‐permeable peptide corresponding the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) Spike C‐terminal domain (CD) inhibits interaction between viral spike nucleocapsid proteins results in SARS‐CoV‐2 replication vitro. Here, we used docking studies design R‐t‐Spike CD(D), more potent short cell‐penetrating composed all D‐form amino acids evaluated its inhibitory effect against S clade other variants. CD(D) was internalized into Vero cells Calu‐3 suppressed clade, delta variant, omicron variant with higher potency than original peptide. In hemizygous K18‐hACE2 mice, intratracheal administration effectively delivered trachea lungs, whereas intranasal mostly upper system stomach, small amount lungs. Administration by either route reduced loads mouse lungs turbinates. Furthermore, intranasally administered mitigated pathological change increased survival mice after infection or variant. Our data suggest has potential as agent infection.

Язык: Английский

Процитировано

12
Comparative analysis of machine learning-based approaches for identifying therapeutic peptides targeting SARS-CoV-2 DOI Creative Commons
Balachandran Manavalan, Shaherin Basith, Gwang Lee

и другие.

Briefings in Bioinformatics, Год журнала: 2021, Номер 23(1)

Опубликована: Сен. 9, 2021

Coronavirus disease 2019 (COVID-19) has impacted public health as well societal and economic well-being. In the last two decades, various prediction algorithms tools have been developed for predicting antiviral peptides (AVPs). The current COVID-19 pandemic underscored need to develop more efficient accurate machine learning (ML)-based rapid identification of therapeutic against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Several peptide-based ML approaches, including anti-coronavirus (ACVPs), IL-6 inducing epitopes other targeting SARS-CoV-2, implemented in therapeutics. Owing growing interest field, it is crucial systematically compare existing based on their performances. Accordingly, we comprehensively evaluated state-of-the-art AVP predictors coronaviruses terms core algorithms, feature encoding schemes, performance evaluation metrics software usability. A comprehensive assessment was then conducted evaluate robustness scalability using well-constructed independent validation datasets. Additionally, discussed advantages disadvantages methods, providing useful insights into development novel computational characterizing identifying or ACVPs. gained from this review are anticipated provide critical guidance scientific community design next-generation silico SARS-CoV-2.

Язык: Английский

Процитировано

26