The Journal of Physical Chemistry Letters,
Год журнала:
2024,
Номер
unknown, С. 12293 - 12300
Опубликована: Дек. 5, 2024
Fluorescence
probes
play
crucial
roles
in
unraveling
the
structure
and
dynamics
of
cell
membranes
including
membrane
fluidity,
polarity,
lipid
molecule
ordering.
The
fluorescence
lifetime
describes
average
duration
time
that
a
fluorescent
remains
an
excited
state
before
returning
to
ground
state,
which
is
sensitive
environmental
changes.
However,
molecular
mechanism
inherent
properties
determine
lifetimes
remain
unexplored
inadequately
studied.
Furthermore,
effects
probe
on
are
also
unclear.
In
this
study,
we
investigated
interactions
between
lipids,
as
well
structural
within
outer
inner
Mycobacterium
smegmatis
(Msm)
by
combining
(MD)
simulations,
enhanced
sampling
methods,
imaging
microscopy
(FLIM),
time-correlated
single
photon
counting
(TCSPC).
results
show
even
though
have
very
little
effect
different
environments
significantly
affect
probes.
analysis
based
all-atom
simulations
shows
strong
correlation
probe's
immersion
depth
its
lifetime.
Specifically,
buried
environment
shielded
from
rapid
water
collisions
exhibit
longer
lifetimes.
basis
revealed
work
would
enhance
comprehension
facilitate
rational
design
novel
efficient
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 10, 2025
Abstract
Mitochondria
play
critical
roles
in
regulating
cell
fate,
with
dysfunction
correlating
the
development
of
multiple
diseases,
emphasizing
need
for
engineered
nanomedicines
that
cross
biological
barriers.
Said
often
target
fluctuating
mitochondrial
properties
and/or
present
inefficient/insufficient
cytosolic
delivery
(resulting
poor
overall
activity),
while
many
require
complex
synthetic
procedures
involving
targeting
residues
(hindering
clinical
translation).
The
synthesis/characterization
polypeptide‐based
penetrating
diblock
copolymers
poly‐L‐ornithine
(PLO)
and
polyproline
(PLP)
(PLO
n
‐PLP
m
,
n:m
ratio
1:3)
are
described
as
mitochondria‐targeting
nanocarriers.
Synthesis
involves
a
simple
two‐step
methodology
based
on
N‐carboxyanhydride
ring‐opening
polymerization,
scale‐up
optimization
using
“design
experiments”
approach.
molecular
mechanisms
behind
targetability
therapeutic
activity
investigated
through
physical/biological
processes
themselves
or
moieties
poly‐L‐glutamic
(PGA)‐based
conjugate.
Diblock
prompt
rapid
entry
via
energy‐independent
recognize
mitochondria
mitochondria‐specific
phospholipid
cardiolipin
(CL).
Stimuli‐driven
conditions
polarization
dynamics,
which
decrease
efficacy
depending
disease
type/stage,
do
not
compromise
copolymer
uptake/targetability.
exhibit
inherent
concentration‐dependent
anti‐tumorigenic
at
level.
conjugate
possesses
improved
safety,
significant
penetration,
accumulation
recognition.
These
findings
may
support
efficient
safe
mitochondrial‐targeting
nanomedicines.
Biophysical Journal,
Год журнала:
2024,
Номер
123(12), С. 1592 - 1609
Опубликована: Май 3, 2024
Sensing
of
the
biophysical
properties
membranes
using
molecular
reporters
has
recently
regained
widespread
attention.
This
was
elicited
by
development
new
probes
exquisite
optical
and
increased
performance,
combined
with
developments
in
fluorescence
detection.
Here,
we
report
on
lifetime
imaging
various
rigid
flexible
fluorescent
dyes
to
probe
synthetic
biological
at
steady
state
as
well
upon
action
external
membrane-modifying
agents.
We
tested
solvatochromic
Nile
red
1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl)
(ammonium
salt)
(NBD),
viscosity
sensor
Bodipy
C
Journal of Cell Science,
Год журнала:
2024,
Номер
137(20)
Опубликована: Сен. 11, 2024
Environment-sensitive
probes
are
frequently
used
in
spectral
and
multi-channel
microscopy
to
study
alterations
cell
homeostasis.
However,
the
few
open-source
packages
available
for
processing
of
images
limited
scope.
Here,
we
present
VISION,
a
stand-alone
software
based
on
Python
analysis
with
improved
applicability.
In
addition
classical
intensity-based
analysis,
our
can
batch-process
multidimensional
an
advanced
single-cell
segmentation
capability
apply
user-defined
mathematical
operations
spectra
calculate
biophysical
metabolic
parameters
single
cells.
VISION
allows
3D
temporal
mapping
properties
such
as
membrane
fluidity
mitochondrial
potential.
We
demonstrate
broad
applicability
by
applying
it
effect
various
drugs
cellular
properties.
correlation
between
potential,
protein
distribution
cell-cell
contacts
nanodomains
cell-derived
vesicles.
Together
code,
provide
graphical
user
interface
easy
adoption.
Faraday Discussions,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
We
use
atomistic
simulations
of
lipid
bilayers
to
generate
representative
datasets
from
model
membranes
in
vitro
,
and
examine
the
sensitivity
various
experimental
techniques
abundance
imbalances
compositionally
asymmetric
bilayers.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 16, 2024
Abstract
Tension
propagates
in
lipid
bilayers
over
hundreds
of
microns
within
milliseconds,
precluding
the
formation
tension
gradients.
Nevertheless,
plasma
membrane
gradients
have
been
evidenced
migrating
cells
and
along
axons.
Here,
using
a
fluorescent
probe,
we
show
that
exist
all
adherent
cells,
whether
they
migrate
or
not.
Non-adhering
do
not
display
We
further
branched
actin
increases
tension,
while
membrane-to-cortex
attachments
facilitate
its
propagation.
is
lowest
at
edge
adhesion
sites
highest
protrusions,
setting
boundaries
By
providing
quantitative
mechanistic
basis
behind
organization
gradients,
our
work
explains
how
are
actively
sustained
cells.
Analytical Methods,
Год журнала:
2024,
Номер
16(32), С. 5509 - 5518
Опубликована: Янв. 1, 2024
This
review
introduces
various
studies
on
cell-sized
liposomes,
which
facilitate
observation
for
the
rapid
detection
and
analysis
of
diverse
materials
based
their
size,
dynamic
shape
changes,
phase
separation,
providing
valuable
insights.
