Transcriptional profiling of exosomes derived from serum of patients with rare earth pneumoconiosis by RNA-sequencing and PI3K/Akt pathway is activated in lung of mice exposed to rare earth Nd2O3.
Toxicology Letters,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Язык: Английский
How mass spectrometry can be exploited to study AMPK
Essays in Biochemistry,
Год журнала:
2024,
Номер
68(3), С. 283 - 294
Опубликована: Июль 26, 2024
AMP-activated
protein
kinase
(AMPK)
is
a
key
regulator
of
metabolism
and
recognised
target
for
the
treatment
metabolic
diseases
such
as
Type
2
diabetes
(T2D).
Here,
we
review
how
mass
spectrometry
(MS)
can
be
used
to
study
short-term
control
by
AMPK
via
phosphorylation
long-term
due
changes
in
expression.
We
discuss
MS
quantify
subunit
levels
tissues
from
different
species.
propose
hydrogen-deuterium
exchange
(HDX)-MS
investigate
molecular
mechanisms
activation
thermoproteomic
profiling
(TPP)
assess
off-target
effects
pharmacological
activators/inhibitors.
Lastly,
because
large
data
sets
are
generated,
consider
approaches
that
their
interpretation.
Язык: Английский
Integrated single cell functional-proteomic profiling of human skeletal muscle reveals a shift in cellular specificity in nemaline myopathy
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 17, 2024
Abstract
Skeletal
muscle
is
a
complex
syncytial
arrangement
of
an
array
cell
types
and,
in
the
case
specific
cells
(myofibers),
sub-types.
There
exists
extensive
heterogeneity
skeletal
functional
behaviour
and
molecular
landscape,
at
composition,
myofiber
sub-type
intra-myofiber
level.
This
highlights
limitations
currently
applied
methodological
approaches,
which
has
stagnated
our
understanding
fundamental
biology
both
healthy
myopathic
contexts.
Here,
we
developed
novel
approach
that
combines
fluorescence
based
assay
for
biophysical
examination
sarcomeric
protein,
myosin,
coupled
with
same-myofiber
high
sensitivity
proteome
profiling,
termed
Single
Myofiber
Protein
Function-Omics
(SMPFO).
Successfully
applying
this
to
human
tissue,
identify
integrate
relationship
between
functionality
underlying
proteomic
landscape
guides
divergent,
but
physiologically
important,
By
SMPFO
two
forms
nemaline
myopathy
(
ACTA1
TNNT1
mutations),
reveal
significant
reduction
divergence
sub-types,
across
behaviour.
Collectively,
develop
as
study
greater
specificity,
accuracy
resolution
then
methods,
facilitating
advancement
SkM
tissue
diseased
states.
Язык: Английский
The ARK2N (C18ORF25) Genetic Variant Is Associated with Muscle Fiber Size and Strength Athlete Status
Metabolites,
Год журнала:
2024,
Номер
14(12), С. 684 - 684
Опубликована: Дек. 5, 2024
Background:
Data
on
the
genetic
factors
contributing
to
inter-individual
variability
in
muscle
fiber
size
are
limited.
Recent
research
has
demonstrated
that
mice
lacking
Arkadia
(RNF111)
N-terminal-like
PKA
signaling
regulator
2N
(Ark2n;
also
known
as
C18orf25)
gene
exhibit
reduced
size,
contraction
force,
and
exercise
capacity,
along
with
defects
calcium
handling
within
fast-twitch
fibers.
However,
role
of
ARK2N
human
physiology,
particularly
athletic
populations,
remains
poorly
understood.
The
aim
this
study
was
threefold:
(a)
compare
expression
between
power
endurance
athletes;
(b)
analyze
relationship
composition;
(c)
investigate
association
functional
variant
gene,
sport-related
phenotypes.
Results:
We
found
significantly
higher
athletes
compared
(p
=
0.042)
positively
associated
proportion
oxidative
(type
IIA)
fibers
untrained
subjects
0.017,
adjusted
for
age
sex).
Additionally,
we
observed
rs6507691
T
allele,
which
predicts
high
3.8
×
10−12),
a
greater
cross-sectional
area
strength
0.015)
over-represented
world-class
(38.6%;
OR
2.2,
p
0.023)
wrestlers
(33.8%;
1.8,
0.044)
controls
(22.0%).
Conclusions:
In
conclusion,
appears
be
specific
myofibers,
its
being
strength-athlete
status.
Язык: Английский