Digital spatial profiling identifies the tumor center as a topological niche in prostate cancer characterized by an upregulation of BAD DOI Creative Commons
Ann‐Kathrin Huber,

Adam Kaczorowski,

F Schneider

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Авг. 31, 2024

Abstract Prostate cancer is characterized by a high degree of intratumoral heterogeneity. However, little known about the spatial distribution cells with respect to specific functional characteristics and formation niches. Here, we used digital profiling (DSP) investigate differences in protein expression tumor center versus periphery. Thirty-seven regions interest were analyzed for 47 proteins, which included components PI3K-AKT, MAPK, cell death signaling pathways as well immune markers. A total 1739 data points collected from five patients. DSP identified BCL-2 associated agonist (BAD) most significantly upregulated center. BAD upregulation was confirmed conventional immunohistochemistry, furthermore showed phosphorylation at serine 112 indicating its inactivation. Knockdown prostate vitro led decreased viability colony growth. Clinically, shorter time biochemical recurrence 158 mostly high-risk Collectively, our results suggest that topological niche may drive progression.

Язык: Английский

Digital spatial profiling identifies the tumor center as a topological niche in prostate cancer characterized by an upregulation of BAD DOI Creative Commons
Ann‐Kathrin Huber,

Adam Kaczorowski,

F Schneider

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Авг. 31, 2024

Abstract Prostate cancer is characterized by a high degree of intratumoral heterogeneity. However, little known about the spatial distribution cells with respect to specific functional characteristics and formation niches. Here, we used digital profiling (DSP) investigate differences in protein expression tumor center versus periphery. Thirty-seven regions interest were analyzed for 47 proteins, which included components PI3K-AKT, MAPK, cell death signaling pathways as well immune markers. A total 1739 data points collected from five patients. DSP identified BCL-2 associated agonist (BAD) most significantly upregulated center. BAD upregulation was confirmed conventional immunohistochemistry, furthermore showed phosphorylation at serine 112 indicating its inactivation. Knockdown prostate vitro led decreased viability colony growth. Clinically, shorter time biochemical recurrence 158 mostly high-risk Collectively, our results suggest that topological niche may drive progression.

Язык: Английский

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