Delineation of Subcellular Molecular Heterogeneity in Single Cells via Ultralow-Flow-Rate Desorption Electrospray Ionization Mass Spectrometry Imaging DOI
Hua Zhang, Xudong Shi, Haiyan Lu

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 4, 2025

Single-cell analysis uncovers cellular heterogeneity and dynamic metabolism at the individual cell level, providing fundamental insights into physiological pathological mechanisms of life. Spatial molecular mapping single cells requires advanced techniques that achieve high spatial resolution comprehensive coverage while preserving cells' native state with minimal preparation. Here, we report an ultralow-flow-rate desorption electrospray ionization mass spectrometry imaging (u-DESI-MSI) platform for in situ biomolecule subcellular resolution. The u-DESI-MSI single-cell utilizes a stable ultralow-solvent-flow-rate (150 nL/min) system optimized geometrical settings, avoiding complicated hardware modifications on ESI emitter. capability was demonstrated by human pancreatic from different lineages. An unprecedented achieved under rastering step size 5 μm, even revealing lipid distribution regions. Using this high-spatial-resolution platform, effectively visualized both intercellular intracellular cells. Notably, provides versatile tool direct their states ambient conditions, eliminating need extensive sample We anticipate will facilitate exploration heterogeneity, offering valuable variability metabolism.

Язык: Английский

Navigating the challenges of engineering composite specialized metabolite pathways in plants DOI Creative Commons
Sachin A. Gharat, Vaijayanti A. Tamhane, Ashok P. Giri

и другие.

The Plant Journal, Год журнала: 2025, Номер 121(6)

Опубликована: Март 1, 2025

SUMMARY Plants are a valuable source of diverse specialized metabolites with numerous applications. However, these compounds often produced in limited quantities, particularly under unfavorable ecological conditions. To achieve sufficient levels target metabolites, alternative strategies such as pathway engineering heterologous systems like microbes (e.g., bacteria and fungi) or cell‐free can be employed. Another approach is plant engineering, which aims to either enhance the native production original reconstruct model system. Although increasing metabolite promising strategy, plants exotic pose significant challenges for genetic manipulation. Effective requires comprehensive prior knowledge genes enzymes involved, well precursor, intermediate, branching, final metabolites. Thus, thorough elucidation biosynthetic closely linked successful metabolic host systems. In this review, we highlight recent advances engineering. We focus on efforts engineer complex, multi‐step pathways that require expression at least eight transient three stable transformation. Reports complex stably transformed remain relatively scarce. discuss major hurdles overcoming them, followed by an overview achievements, challenges, solutions reconstitution through Recent including computer‐based predictions offer platforms sustainable plants.

Язык: Английский

Процитировано

1
Single-cell mass spectrometry imaging of lipids in HeLa cells via tapping-mode scanning probe electrospray ionization DOI Creative Commons
Yoichi Otsuka, Kazuya Kabayama,

Ayane Miura

и другие.

Communications Chemistry, Год журнала: 2025, Номер 8(1)

Опубликована: Май 14, 2025

Язык: Английский

Процитировано

0
Linking oxidative stress biomarkers to disease progression and antioxidant therapy in hypertension and diabetes mellitus DOI Creative Commons

Alberto J. Nuñez-Selles,

Rodolfo A. Nuñez-Musa,

Rafael A. Guillen-Marmolejos

и другие.

Frontiers in Molecular Biosciences, Год журнала: 2025, Номер 12

Опубликована: Май 26, 2025

Oxidative stress (OS) is increasingly recognized as a key factor linking hypertension (HTN) and diabetes mellitus (DM). This review summarizes recent evidence regarding the dual role of OS both an instigator amplifier cardiometabolic dysfunction. In HTN, reactive oxygen species (ROS) produced by NADPH oxidases (NOXs) mitochondrial dysfunction contribute to endothelial impairment vascular remodeling. DM, hyperglycemia-induced ROS production worsens beta-cell failure insulin resistance through pathways such AGE-RAGE signaling, protein kinase C (PKC) activation, polyol pathway. Clinically validated biomarkers OS, F2-isoprostanes (which indicate lipid peroxidation), 8-OHdG indicates DNA damage), activities redox enzymes like superoxide dismutase (SOD) glutathione peroxidase (GPx), show strong correlations with disease progression end-organ complications. Despite promising preclinical results, application antioxidant therapies in clinical settings has faced challenges due inconsistent outcomes, highlighting need for targeted approaches. Emerging strategies include: 1. Mitochondria-targeted antioxidants enhance function resistant HTN; 2. Nrf2 activators restore balance early diabetes; 3. Specific inhibitors NOX isoforms. We emphasize three transformative areas research: (i) interaction between microbiome ROS, where modifying gut microbiota can reduce systemic OS; (ii) use nanotechnology deliver directly pancreatic islets or atherosclerotic plaques; (iii) phenotype-specific diagnosis therapy guided genetic profiling (for example, KEAP1/NRF2 polymorphisms). Integrating these advances lifestyle modifications, following Mediterranean diet exercising regularly, may provide additional benefits. outlines mechanistic framework targeting comorbidity HTN DM while identifying critical knowledge gaps, particularly timing signaling development personalized medicine, which serve reference researchers clinicians working this area.

Язык: Английский

Процитировано

0
Multidimensional single-cell analysis: Diverse strategies and emerging applications in the life sciences DOI
Boyang Zhang,

Xinyue Lan,

Siyuan Tan

и другие.

TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118170 - 118170

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Recent advances in single-cell metabolomics using mass spectrometry: emerging challenges and future perspectives DOI
Rafiqul Alam, Jung‐Hyun Lee, Dasom Shin

и другие.

Applied Spectroscopy Reviews, Год журнала: 2025, Номер unknown, С. 1 - 37

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Delineation of Subcellular Molecular Heterogeneity in Single Cells via Ultralow-Flow-Rate Desorption Electrospray Ionization Mass Spectrometry Imaging DOI
Hua Zhang, Xudong Shi, Haiyan Lu

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 4, 2025

Single-cell analysis uncovers cellular heterogeneity and dynamic metabolism at the individual cell level, providing fundamental insights into physiological pathological mechanisms of life. Spatial molecular mapping single cells requires advanced techniques that achieve high spatial resolution comprehensive coverage while preserving cells' native state with minimal preparation. Here, we report an ultralow-flow-rate desorption electrospray ionization mass spectrometry imaging (u-DESI-MSI) platform for in situ biomolecule subcellular resolution. The u-DESI-MSI single-cell utilizes a stable ultralow-solvent-flow-rate (150 nL/min) system optimized geometrical settings, avoiding complicated hardware modifications on ESI emitter. capability was demonstrated by human pancreatic from different lineages. An unprecedented achieved under rastering step size 5 μm, even revealing lipid distribution regions. Using this high-spatial-resolution platform, effectively visualized both intercellular intracellular cells. Notably, provides versatile tool direct their states ambient conditions, eliminating need extensive sample We anticipate will facilitate exploration heterogeneity, offering valuable variability metabolism.

Язык: Английский

Процитировано

0