Lactobacillus rhamnosus GG as Biosensor for Oral and Systemic Health Conditions: A Pilot Study DOI Creative Commons
Pooja Mali, Andrei C. Ionescu, Alessio Soggiù

и другие.

Applied Sciences, Год журнала: 2025, Номер 15(9), С. 4809 - 4809

Опубликована: Апрель 26, 2025

Early disease detection using biosensors is a significant challenge in modern medicine. This study aimed to investigate Lactobacillus rhamnosus GG (LGG) as bacterial biosensor for biomarkers indicative of oral and systemic health conditions. For this purpose, LGG was cultured then exposed phosphate buffer, 10 wt.% sucrose solution, pH = 4.0, lactic acid, filter-sterile saliva from five subjects. A total groups consisted filter-sterile, freshly pooled subject 1 (SANT), 2 (SLAN), 3 (SLFU), 4 (SLPA), 5 (SLPO), buffer solution (pH 7.4, BUF), PBS with resin. Subsequently, the proteomic profiling samples done by high-resolution mass spectrometry, focusing on expression proteins. The were evaluated biosensing capacity through its expression. Statistical comparisons performed outline changes, clustering upregulated downregulated proteins relevant stress response, metabolism, environmental adaptation. identification key associated metabolic regulation, response oxidative stress, adaptation possible heatmaps volcano plots. Each subject’s salivary composition also presented unique, characteristic signature. Results showed massive downregulation linked under nutrient-rich In conclusion, early protein modifications related niche changes has shown that can serve promising novel diagnostic tool, potentially overcoming many drawbacks current physicochemical transducer-based biosensors.

Язык: Английский

Lactobacillus rhamnosus GG as Biosensor for Oral and Systemic Health Conditions: A Pilot Study DOI Creative Commons
Pooja Mali, Andrei C. Ionescu, Alessio Soggiù

и другие.

Applied Sciences, Год журнала: 2025, Номер 15(9), С. 4809 - 4809

Опубликована: Апрель 26, 2025

Early disease detection using biosensors is a significant challenge in modern medicine. This study aimed to investigate Lactobacillus rhamnosus GG (LGG) as bacterial biosensor for biomarkers indicative of oral and systemic health conditions. For this purpose, LGG was cultured then exposed phosphate buffer, 10 wt.% sucrose solution, pH = 4.0, lactic acid, filter-sterile saliva from five subjects. A total groups consisted filter-sterile, freshly pooled subject 1 (SANT), 2 (SLAN), 3 (SLFU), 4 (SLPA), 5 (SLPO), buffer solution (pH 7.4, BUF), PBS with resin. Subsequently, the proteomic profiling samples done by high-resolution mass spectrometry, focusing on expression proteins. The were evaluated biosensing capacity through its expression. Statistical comparisons performed outline changes, clustering upregulated downregulated proteins relevant stress response, metabolism, environmental adaptation. identification key associated metabolic regulation, response oxidative stress, adaptation possible heatmaps volcano plots. Each subject’s salivary composition also presented unique, characteristic signature. Results showed massive downregulation linked under nutrient-rich In conclusion, early protein modifications related niche changes has shown that can serve promising novel diagnostic tool, potentially overcoming many drawbacks current physicochemical transducer-based biosensors.

Язык: Английский

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