Water-Based Polyurethane Dispersions: A Detailed Analysis of the Particle Charge Using Soft and Hard Particle Model DOI

Christoph Grau,

Annette M. Schmidt, J. Wilkens

и другие.

Langmuir, Год журнала: 2024, Номер 40(42), С. 22123 - 22135

Опубликована: Окт. 9, 2024

Water-based polyurethane dispersions (PUDs) show a characteristic dependency of the electrophoretic mobility on electrolyte concentration, which can be investigated by hard and soft particle models. For this purpose, additional information obtained determining charges electrostatic potentials. PUDs with different contents an intrinsic ionic stabilizer various polyol components were synthesized according to acetone process. The charge was characterized potentiometric acid–base titration, data titration curve fitted assuming multiple functional groups adaptable acid strengths. To investigate potentials, mobilities measured as function concentration analyzed theory. Acid–base experiments indicated that not all detected are located surface but partly arranged inside polymer particle, evidenced significant decrease corresponding effective dissociation constant. evaluation electrokinetic showed model Ohshima is suitable reflect actual charge. In contrast, describe very well if relaxation effect taken into account. zeta potentials excellently modeled constant potential ψ0 distance shear plane xs. Reasonable results for both parameters only minor differences between PUD series. Nonetheless, densities calculated respect lower than expected from results. Although our indicate more complex distribution in peripheral layer, currently shows best description behavior PUDs.

Язык: Английский

Chromatography Media and Purification Processes for Complex and Super-large Biomolecules: A Review DOI

Lan Zhao,

Guanghui Ma

Journal of Chromatography A, Год журнала: 2025, Номер unknown, С. 465721 - 465721

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Interfacial Electrokinetic Phenomena of Thermoresponsive Microgels with a Spatial Gradient of Charged Groups DOI
Yuichiro Nishizawa,

Yuji Sato,

Ryuji Namioka

и другие.

Langmuir, Год журнала: 2025, Номер unknown

Опубликована: Фев. 24, 2025

When functional microgels are synthesized via radical copolymerization, spatial gradients of groups often form due to a difference in reactivity ratios between monomer and comonomer. In this study, we systematically investigated the effect decreasing gradient charged from core shell on their surface properties, which crucial for colloidal particles, through analysis interfacial electrokinetic phenomena using Ohshima's equation. A series electrophoretic analyses combined with dynamic light scattering revealed that undergoes multistep collapse during particle-size reduction dehydration upon increasing temperature. Furthermore, more complicated hierarchical within was elucidated by quantitatively evaluating changes properties precipitation polymerization based phenomena.

Язык: Английский

Процитировано

0
Optimizing nuclease treatment to enhance anion exchange chromatography of HIV-derived virus-like particles DOI Creative Commons

M S von Elling-Tammen,

Florian Taft,

Volkmar Thom

и другие.

Journal of Chromatography B, Год журнала: 2025, Номер 1256, С. 124539 - 124539

Опубликована: Фев. 27, 2025

Residual host cell chromatin imposes numerous challenges on purifying HIV-derived enveloped virus-like particles (VLPs) using anion-exchange chromatography (AEX). According to FDA guidelines, DNA must be reduced less than 10 ng per dose at a fragment size of 200 bp. To prove the fulfillment these quality criteria, methods for qualitative and quantitative analysis fragments applied adapted chromatin. impede purification HIV VLPs with AEX, co-eluting in same fractions as VLPs. Although nuclease treatments can employed, structure shield from activity. address challenges, we adjusted our analytical focus characterizing clarified VLP supernatant. We identified two subpopulations: main large population minor consisting short below Our findings demonstrated that larger are primary issue process, they co-elute desired remove long population, optimized treatment Design Experiment approach digest despite tight structure. The nucleases Benzonase, Denarase, M-SAN efficiently digested removing over 90 % DNA. By shredding before AEX step, successfully separated remaining fragments. Combined treatment, membrane offers an efficient single-step platform VLP-based vaccines other therapeutics.

Язык: Английский

Процитировано

0
Water-Based Polyurethane Dispersions: A Detailed Analysis of the Particle Charge Using Soft and Hard Particle Model DOI

Christoph Grau,

Annette M. Schmidt, J. Wilkens

и другие.

Langmuir, Год журнала: 2024, Номер 40(42), С. 22123 - 22135

Опубликована: Окт. 9, 2024

Water-based polyurethane dispersions (PUDs) show a characteristic dependency of the electrophoretic mobility on electrolyte concentration, which can be investigated by hard and soft particle models. For this purpose, additional information obtained determining charges electrostatic potentials. PUDs with different contents an intrinsic ionic stabilizer various polyol components were synthesized according to acetone process. The charge was characterized potentiometric acid–base titration, data titration curve fitted assuming multiple functional groups adaptable acid strengths. To investigate potentials, mobilities measured as function concentration analyzed theory. Acid–base experiments indicated that not all detected are located surface but partly arranged inside polymer particle, evidenced significant decrease corresponding effective dissociation constant. evaluation electrokinetic showed model Ohshima is suitable reflect actual charge. In contrast, describe very well if relaxation effect taken into account. zeta potentials excellently modeled constant potential ψ0 distance shear plane xs. Reasonable results for both parameters only minor differences between PUD series. Nonetheless, densities calculated respect lower than expected from results. Although our indicate more complex distribution in peripheral layer, currently shows best description behavior PUDs.

Язык: Английский

Процитировано

1