Advances with Lipid-Based Nanosystems for siRNA Delivery to Breast Cancers
Pharmaceuticals,
Год журнала:
2023,
Номер
16(7), С. 970 - 970
Опубликована: Июль 6, 2023
Breast
cancer
is
the
most
frequently
diagnosed
among
women.
also
key
reason
for
worldwide
cancer-related
deaths
The
application
of
small
interfering
RNA
(siRNA)-based
drugs
to
combat
breast
requires
effective
gene
silencing
in
tumor
cells.
To
overcome
challenges
drug
delivery
tumors,
various
nanosystems
siRNA
delivery,
including
lipid-based
nanoparticles
that
protect
from
degradation
cells
have
been
developed.
These
shown
great
potential
efficient
and
targeted
Lipid-based
remain
promising
as
carriers
safe
therapy
cancer.
Lipid
(LNPs)
encapsulating
enable
specific
oncogenes
tumors.
This
review
discusses
a
variety
cationic
lipids,
sterols,
phospholipids,
PEG-lipid
conjugates,
ionizable
liposomes,
exosomes
clinical
translation
solid
Язык: Английский
Phosphorodiamidate Morpholino Oligomers-Loaded Nanobubbles for Ultrasound-Mediated Delivery to the Myocardium in Muscular Dystrophy
ACS Omega,
Год журнала:
2025,
Номер
10(9), С. 9639 - 9648
Опубликована: Фев. 27, 2025
Microbubbles
(MBs)
and
nanobubbles
(NBs)
can
oscillate
collapse
in
response
to
ultrasound
exposure,
resulting
contrast
delivery
effects.
Therefore,
the
retention
of
entrapped
gas
is
an
important
condition
bubble
formulations,
especially
for
MBs
NBs
with
lipid
shells,
stability
membrane
considered
be
affected.
We
previously
developed
NBs,
which
are
polyethylene
glycol-modified
liposomes
entrapping
that
serve
as
nucleic
acid
carriers
agents.
In
particular,
containing
cationic
lipids
were
useful
systemic
tools
load
genes
acids
on
their
surfaces.
However,
low,
leaving
room
improvement
this
study,
we
attempted
prepare
anionic
improve
vivo,
found
they
lasted
longer
time
than
previous
NBs.
order
utilize
evaluated
usefulness
cationic-peptide-conjugated
phosphorodiamidate
morpholino
oligomers
(PMO).
PMO
has
attracted
attention
a
therapeutic
agent
Duchenne
muscular
dystrophy
(DMD);
however,
its
charge
neutrality
makes
into
muscle
fibers
challenging,
more
difficult
apply
myocardial
damage.
examined
heart
using
combination
ultrasound.
Furthermore,
usability
octaarginine
(R8),
cell-penetrating
peptide
(CPP),
loading
onto
surface
verified
potential
PMO-loaded
therapy
cardiac
dysfunction
dystrophy.
Язык: Английский
mRNA Sonotransfection of Tumors with Polymeric Microbubbles: Co‐Formulation versus Co‐Administration
Advanced Science,
Год журнала:
2024,
Номер
11(15)
Опубликована: Фев. 11, 2024
Abstract
Despite
its
high
potential,
non‐viral
gene
therapy
of
cancer
remains
challenging
due
to
inefficient
nucleic
acid
delivery.
Ultrasound
(US)
with
microbubbles
(MB)
can
open
biological
barriers
and
thus
improve
DNA
mRNA
passage.
Polymeric
MB
are
an
interesting
alternative
clinically
used
lipid‐coated
because
their
stability,
narrow
size
distribution,
easy
functionalization.
However,
besides
choosing
the
ideal
MB,
it
unclear
whether
nanocarrier‐encapsulated
should
be
administered
separately
(co‐administration)
or
conjugated
(co‐formulation).
Therefore,
impact
poly(n‐butyl
cyanoacrylate)
co‐administration
mRNA‐DOTAP/DOPE
lipoplexes
co‐formulation
on
transfection
cells
in
vitro
vivo
is
analyzed.
Sonotransfection
improved
delivery
into
4T1
breast
being
more
efficient
than
co‐formulation.
In
vivo,
sonotransfection
approach
also
resulted
higher
efficiency
reached
deeper
tumor
tissue.
On
contrary,
mainly
promoted
endothelial
perivascular
cells.
Furthermore,
much
dependent
US
trigger,
resulting
significantly
lower
off‐site
transfection.
Thus,
findings
indicate
that
choice
depend
targeted
cell
population,
tolerable
transfection,
therapeutic
purpose.
Язык: Английский
Brief Comparison of the Efficacy of Cationic and Anionic Liposomes as Nonviral Delivery Systems
ACS Omega,
Год журнала:
2024,
Номер
9(47), С. 46664 - 46678
Опубликована: Ноя. 13, 2024
In
recent
decades,
the
development
and
application
of
nonviral
vectors,
such
as
liposomes
lipidic
nanoparticles,
for
gene
therapy
drug
delivery
have
seen
substantial
progress.
The
interest
in
physicochemical
properties
structures
complexes
liposome/DNA
liposome/RNA
is
due
to
their
potential
substitute
viruses
carriers
drugs
or
genetic
material
into
cells
with
minimal
cytotoxicity,
which
could
lead
use
therapy.
Initially,
cationic
were
utilized
DNA
vectors;
subsequently,
different
molecules,
polymers,
incorporated
enhance
transfection
efficiency.
Additionally,
liposome/protein
been
developed
vectors
treatment
diseases.
most
relevant
internalization
pathways
these
few
results
obtained
using
targeted
nontargeted
are
discussed
below.
high
cytotoxicity
represents
a
significant
challenge
delivery.
Anionic
offer
promising
alternative
address
limitations
conventional
liposomes,
including
immune
response,
short
circulation
time,
low
toxicity.
This
review
will
discuss
advantages
novel
anionic
liposome-based
systems
that
emerged
result.
advent
designs
manufacturing
techniques
has
facilitated
innovative
systems,
designated
lipid
nanoparticles
(LNPs),
serve
highly
efficacious
regulators
system.
Язык: Английский