The
study
of
individual
proteins
in
their
natural
state
without
the
use
fluorescent
labels
is
vital
for
gaining
a
comprehensive
understanding
behavior
and
function
vivo.
However,
traditional
fluorescence
labeling
methods
can
alter
structure
proteins,
leading
to
inaccurate
data.
To
overcome
this
limitation,
our
team
has
developed
revolutionary
label-free
detection
platform
single
ultraviolet
(UV)
range.
This
utilizes
UV
autofluorescence
relies
on
new
optical
horn
antenna
design,
which
allows
unprecedented
resolution
sensitivity.
While
current
technology
state-of-the-art,
it
still
limitations,
such
as
limited
enhancement
photon
count
rate
10–15
times
higher
than
confocal
[1],
[2].
achieve
even
greater
enhancement,
we
are
exploring
rhodium
cube
gap
self-assembled
using
capillary-convective
forces
inside
rectangular
zero-mode
waveguide.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Янв. 2, 2024
Abstract
Single
molecule
fluorescence
spectroscopy
is
at
the
heart
of
molecular
biophysics
research
and
most
sensitive
biosensing
assays.
The
growing
demand
for
precision
medicine
environmental
monitoring
requires
creation
miniaturized
portable
sensing
platforms.
However,
need
highly
sophisticated
objective
lenses
has
precluded
development
single
detection
systems
truly
devices.
Here,
we
propose
a
dielectric
metalens
device
submicrometer
thickness
to
excite
collect
light
from
fluorescent
molecules
instead
an
lens.
high
numerical
aperture,
focusing
efficiency,
dual-wavelength
operation
enable
implementation
correlation
with
Alexa
647
in
focal
volume.
Moreover,
enables
real-time
individual
nanoparticle
transitions
identification
hydrodynamic
diameters
ranging
few
hundreds
nanometers.
This
advancement
sensitivity
extends
application
technology
ultracompact
single-molecule
sensors.
Intraoperative
histology
is
essential
for
surgical
guidance
and
decision-making.
However,
frozen-sectioned
hematoxylin
eosin
(H&E)
staining
suffers
from
degraded
accuracy,
whereas
the
gold-standard
formalin-fixed
paraffin-embedded
(FFPE)
H&E
too
lengthy
intraoperative
use.
Stimulated
Raman
scattering
(SRS)
microscopy
has
shown
rapid
of
brain
tissue
with
lipid/protein
contrast
but
challenging
to
yield
images
identical
nucleic
acid-/protein-based
FFPE
stains
interpretable
pathologists.
Here,
we
report
development
a
semi-supervised
stimulated
CycleGAN
model
convert
fresh-tissue
SRS
using
unpaired
training
data.
Within
3
minutes,
virtual
(SRVH)
results
that
matched
perfectly
true
could
be
generated.
A
blind
validation
indicated
board-certified
neuropathologists
are
able
differentiate
histologic
subtypes
human
glioma
on
SRVH
hardly
conventional
images.
may
provide
diagnosis
superior
frozen
in
both
speed
extendable
other
types
solid
tumors.
Photoacoustics,
Год журнала:
2023,
Номер
32, С. 100545 - 100545
Опубликована: Авг. 1, 2023
Photoacoustic
microscopy
is
advancing
with
research
on
utilizing
ultraviolet
and
visible
light.
Dual-wavelength
approaches
are
sought
for
observing
DNA/RNA-
vascular-related
disorders.
However,
the
availability
of
high
numerical
aperture
lenses
covering
both
wavelengths
severely
limited
due
to
challenges
such
as
chromatic
aberration
in
optics.
Herein,
we
present
a
groundbreaking
proposal
pioneering
simulation
study
incorporating
multilayer
metalenses
into
ultraviolet-visible
photoacoustic
microscopy.
The
proposed
metalens
has
thickness
1.4
µm
0.8.
By
arranging
cylindrical
hafnium
oxide
nanopillars,
design
an
achromatic
transmissive
lens
266
532
nm
wavelengths.
achieves
diffraction-limited
focal
spot,
surpassing
commercially
available
objective
lenses.
Through
three-dimensional
simulation,
demonstrate
high-resolution
imaging
superior
endogenous
contrast
targets
optical
absorption
bands.
This
will
open
new
possibilities
downsized
multispectral
clinical
preclinical
applications.
Frontiers in Chemistry,
Год журнала:
2024,
Номер
12
Опубликована: Июнь 26, 2024
Fluorescence
spectroscopy
serves
as
an
ultrasensitive
sophisticated
tool
where
background
noises
which
serve
a
major
impediment
to
the
detection
of
desired
signals
can
be
safely
avoided
for
detections
down
single-molecule
levels.
One
such
way
bypassing
noise
is
plasmon-enhanced
fluorescence
(PEF),
interactions
fluorophores
at
surface
metals
or
plasmonic
nanoparticles
are
probed.
The
underlying
condition
significant
spectral
overlap
between
localized
plasmon
resonance
(LSPR)
nanoparticle
and
absorption
emission
spectra
fluorophore.
rationale
being
coupling
excited
state
fluorophore
with
leads
augmented
emission,
owing
local
field
enhancement.
It
manifested
in
enhanced
quantum
yields
concurrent
decrease
lifetimes,
increase
radiative
rate
constants.
This
improvement
provided
by
PEF
allows
scope
expansion
domain
weakly
emitting
otherwise
would
have
remained
unperceivable.
concept
weak
emitters
plasmons
bypass
problems
photobleaching,
opening
up
avenues
imaging
significantly
higher
sensitivity
improved
resolution.
Furthermore,
amplification
signal
free
electrons
metal
provides
ample
opportunities
achieving
lower
limits
that
involved
biological
molecular
sensing.
avenue
has
attracted
attraction
last
few
years
fast,
label-free
bio-analytes
under
physiological
conditions
using
point-of-care
analysis.
review
focusses
on
applications
nanomaterials
biosensing,
brief
introduction
different
aspects
LSPR
fabrication
techniques.
Advanced Optical Materials,
Год журнала:
2023,
Номер
11(13)
Опубликована: Апрель 7, 2023
Abstract
Single‐molecule
fluorescence
techniques
are
essential
for
investigating
the
molecular
mechanisms
in
biological
processes.
However,
achieving
sub‐millisecond
temporal
resolution
to
monitor
fast
dynamics
remains
a
significant
challenge.
The
brightness
is
key
parameter
that
generally
defines
these
techniques.
Conventional
microscopes
and
standard
fluorescent
emitters
fall
short
high
required
monitoring.
Plasmonic
nanoantennas
proposed
as
solution,
but
despite
huge
enhancement
having
been
obtained
with
structures,
below
1
million
photons/s/molecule.
Therefore,
improvement
of
overlooked.
This
article
presents
method
single‐molecule
using
plasmonic
nanoantennas,
specifically
optical
horn
antennas.
work
demonstrates
about
90%
collection
efficiency
total
emitted
light,
reaching
2
photons/s/molecule
saturation
regime.
enables
observations
single
molecules
microsecond
binning
time
correlation
spectroscopy
measurements.
expands
applications
antennas
zero‐mode
waveguides
regime
toward
brighter
signal,
faster
resolutions,
improved
detection
rates
advance
sensing,
DNA
sequencing,
dynamic
studies
interactions.
Optics Express,
Год журнала:
2023,
Номер
31(8), С. 12162 - 12162
Опубликована: Март 13, 2023
Hyperlenses
offer
an
appealing
opportunity
to
unlock
bioimaging
beyond
the
diffraction
limit
with
conventional
optics.
Mapping
hidden
nanoscale
spatiotemporal
heterogeneities
of
lipid
interactions
in
live
cell
membrane
structures
has
been
accessible
only
using
optical
super-resolution
techniques.
Here,
we
employ
a
spherical
gold/silicon
multilayered
hyperlens
that
enables
sub-diffraction
fluorescence
correlation
spectroscopy
at
635
nm
excitation
wavelength.
The
proposed
focusing
Gaussian
diffraction-limited
beam
below
40
nm.
Despite
pronounced
propagation
losses,
quantify
energy
localization
inner
surface
determine
(FCS)
feasibility
depending
on
resolution
and
field
view.
We
simulate
diffusion
FCS
function
demonstrate
reduction
time
fluorescent
molecules
up
nearly
2
orders
magnitude
as
compared
free
space
excitation.
show
can
effectively
distinguish
transient
trapping
sites
simulated
2D
membranes.
Altogether,
versatile
fabricable
platforms
display
pertinent
applicability
for
enhanced
reveal
biological
dynamics
single
molecules.
