A DNA Machine-Based Magnetic Resonance Imaging Nanoprobe for In Vivo MicroRNA Detection DOI
Sijie Song, Qi Wang,

Jiangao Xie

и другие.

Talanta, Год журнала: 2024, Номер unknown, С. 126867 - 126867

Опубликована: Сен. 1, 2024

Язык: Английский

Polyoxometalates: Cascading Functionality to Unique On‐Off Organic Photoelectrochemical Transistor Operation DOI
Feng‐Zao Chen,

Lu Hou,

Yuan Gao

и другие.

Advanced Functional Materials, Год журнала: 2024, Номер 34(48)

Опубликована: Июнь 29, 2024

Abstract While existing polyoxometalates (POMs) are predominantly utilized in a range of energy devices, their potential bioelectronics and optoelectronics, particularly organic photoelectrochemical transistors (OPECTs), remains unexplored. Here, the POMs this aspect is unveiled by POM/sulfide‐gated OPECT. The representative PW 12 /ZnIn 2 S 4 with cascading light‐harvesting enzyme‐like functionality enables unique on‐off OPECT operation. incorporation facilitates separation photogenerated charge carriers across heterojunction interface, consequently inducing pronounced gating effect. Linking CRISPR/Cas13a system correlating target miRNA‐21 biological generation H O , will enable polymerization dopamine into polydopamine thus sensitively inhibit gating. Experimental results demonstrated that developed sensor achieved good analytical performance detection limit as low 3.9 f m . This study demonstrates Given large family multifunction POMs, its implications diverse optoelectronics expected.

Язык: Английский

Процитировано

34

A Smartphone-Mediated “All-In-One” Biosensing Chip for Visual and Value-Assisted Detection DOI
Jing Xu, Yujin Li, Futing Wang

и другие.

Analytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Сен. 20, 2024

A smartphone-mediated self-powered biosensor is fabricated for miRNA-141 detection based on the CRISPR/Cas12a cross-cutting technique and a highly efficient nanozyme. As novel nanozyme signal-amplified coreaction accelerator, AuPtPd@GDY exhibits an excellent ability to catalyze cascade color reactions high conductivity enhance electrochemical signal assays. After of S2-glucose oxidase (S2-GOD), weakened, detected by monitoring decrease in signal. On other hand, reaction among glucose, H

Язык: Английский

Процитировано

11

Advances in CRISPR/Cas13a-based biosensors for non-coding RNA detection DOI
Tao Zhu, Weiwei Jiang,

Yingyu Wu

и другие.

Talanta, Год журнала: 2025, Номер 294, С. 128223 - 128223

Опубликована: Апрель 25, 2025

Язык: Английский

Процитировано

1

Iontronic Sensing Based on Confined Ion Transport DOI
Saud Asif Ahmed, Ying Liu, Tianyi Xiong

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 96(20), С. 8056 - 8077

Опубликована: Апрель 25, 2024

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTIontronic Sensing Based on Confined Ion TransportSaud Asif AhmedSaud AhmedBeijing National Laboratory for Molecular Science, CAS Key of Analytical Chemistry Living Biosystems, Institute Chemistry, Chinese Academy Sciences, Beijing 100190, ChinaMore by Saud AhmedView BiographyView Biography, Ying LiuYing LiuBeijing ChinaUniversity Liu, Tianyi XiongTianyi XiongBeijing Xiong, Yueru ZhaoYueru ZhaoBeijing Zhao, Boyang XieBoyang XieBeijing Xie, Cong PanCong PanBeijing Pan, Wenjie MaWenjie MaBeijing Ma, and Ping Yu*Ping YuBeijing China*[email protected]More Yuhttps://orcid.org/0000-0002-6096-1933Cite this: Anal. Chem. 2024, 96, 20, 8056–8077Publication Date (Web):April 25, 2024Publication History Received12 March 2024Accepted15 April 2024Revised12 2024Published online25 inissue 21 May 2024https://pubs.acs.org/doi/10.1021/acs.analchem.4c01354https://doi.org/10.1021/acs.analchem.4c01354review-articleACS PublicationsCopyright © 2024 American Chemical SocietyRequest reuse permissionsArticle Views638Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking donut icon will load page at altmetric.com with additional details score social media presence given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Charge transport,Ions,Nanoparticles,Nanopores,Sensors Get e-Alerts

Язык: Английский

Процитировано

5

Programming the Dynamic Range of Nanochannel Biosensors for MicroRNA Detection through Allosteric DNA Probes DOI Open Access
Fan Xia, Zhiwei Shang,

Defang Ding

и другие.

Angewandte Chemie International Edition, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 4, 2024

Abstract Solid‐state nanochannel biosensors are extensively utilized for microRNA (miRNA) detection owing to their high sensitivity and rapid response. However, conventional face limitations in fixed dynamic range, restricting versatility efficacy. Herein, we introduce tunable triblock DNA probes with varying affinities target miRNA engineer solid‐state capable of customizable range adjustment. The architecture comprises a poly‐adenine (polyA) block adjustable surface density anchoring, alongside stem loop blocks modulating structural stability. Through systematic manipulation these blocks, demonstrate the ability achieve diverse binding limits, achieving an initial 81‐fold range. By combining various affinities, extend this significantly 10,900‐fold. Furthermore, by implementing sequestration mechanism, effective biosensor is narrowed only 3‐fold span concentrations. advanced makes them highly promising broad spectrum biomedical clinical applications.

Язык: Английский

Процитировано

3

DNA-Assembled Size-Permeable Microcapsule Bioreactor Enables Direct and Rapid Sensing of Trace Circulating MicroRNA Biomarkers in Undiluted Cancer Patient Serum DOI
Fang Yang,

Shunmei Li,

Xin Bi

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 2, 2025

Sensitive and direct detection of circulating microRNAs (miRNAs) in undiluted human serums can significantly facilitate the diagnosis different diseases early stages; however, achievement such a goal is highly challenged by extremely complex sample matrix nature serums. Here, we show that sensitive rapid let-7a miRNA biomarker cancer patient be realized new size-permeable microcapsule bioreactor. The Cas13a/crRNA-dsDNA@polyethylene glycol core/shell biomicrocapsules are synthesized simple encapsulation etching steps. Such exhibit high stability, enhanced protein antifouling capability, size-selective permeability only allows short sequences to enter core cavities, thereby enabling down 0.21 pM within 20 min via signal amplification Cas13a/crRNA near-infrared (NIR) fluorescence signals. biomicrocapsule also unambiguous discrimination clear cell renal carcinoma patients from healthy groups serum samples, highlighting its robust clinical potential for disease diagnoses.

Язык: Английский

Процитировано

0

CRISPR/Cas-powered “sweet” DNA nano-sponge for highly sensitive detection of pathogen nucleic acid with portable personal glucometer DOI

Huiling You,

Jingwen Shan,

Yang Bai

и другие.

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160618 - 160618

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0

Dual-Functionalized Glass Micropipette Sensor for Simultaneous High Sensitivity Detection of Cancer Biomarkers DOI
Guofeng Wang,

Shiwei Xu,

Yueyue Feng

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Март 26, 2025

Early cancer detection is crucial for improving patient survival rates. However, current single-biomarker methods often face challenges, such as insufficient sensitivity, poor accuracy, and false positives. To address these issues, we report a dual-functionalized glass micropipet sensor (DFMS) capable of simultaneously detecting two biomarkers, nucleic acids proteins. The inner surface the functionalized with amino-modified silicon nanowires (SiNWs) to capture disease-related miRNAs, enabling ionic-current-based detection, while outer decorated gold nanoparticles anchor specific protein aptamers Raman-based detection. This dual-functionalization significantly enhances sensitivity selectivity by combining ionic amplification plasmonic Raman signal enhancement. achieves limits 1 aM miRNAs 0.001 ng/mL proteins, minimal mutual interference between modes, ensuring accurate independent Validation prostate biomarkers miRNA-1246 PSA, well gastric miRNA-106a CD44, demonstrates its outstanding selectivity, stability, broad applicability, providing novel approach early significant clinical implications.

Язык: Английский

Процитировано

0

Ultrasensitive Clinical identification of Hepatitis B surface antigen (HBsAg) by CRISPR-assisted nanopore sensing DOI

Minghan Li,

Cun-wen Tao,

Zhuoyun Tang

и другие.

Biosensors and Bioelectronics, Год журнала: 2025, Номер 286, С. 117579 - 117579

Опубликована: Май 15, 2025

Язык: Английский

Процитировано

0

CRISPR/Cas Multiplexed Biosensing: Advances, Challenges, and Perspectives DOI

Luyu Wei,

Zhilong Wang, Yuanbin She

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 27, 2025

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) protein systems are renowned for their high sensitivity specificity, enabling them as a powerful diagnostic toolbox. Multiplexed detection of panels targets, opposed to single is imperative reliable conclusive disease diagnostics. However, multiplex application the CRISPR/Cas system has long been hindered by indistinguishable signals from specific targets due nonspecific chaotic trans-cleavage. To make breakthrough, substantial efforts have devoted CRISPR/Cas-powered multiplexed biosensing strategies, which consequently experienced rapid development over past five years. This review systematically summarizes recent advances in encompassing Cas9, Cas12, Cas13. Key focus issues include strategies respective advantages limitations, sensing mechanisms, performance novel validated examples. Finally, status challenges critically discussed, future outlooks proposed potential practical application. Perspective aims inspire significant research promote next generation biosensing.

Язык: Английский

Процитировано

0