ACS Sensors,
Год журнала:
2024,
Номер
9(7), С. 3540 - 3548
Опубликована: Июнь 22, 2024
Infection
with
drug-resistant
bacteria
poses
a
significant
threat
to
human
health.
Judicious
use
of
antibiotics
could
reduce
the
likelihood
bacterial
resistance,
which
can
be
evaluated
through
antibiotic
susceptibility
testing
(AST).
This
paper
focuses
on
application
needle-like
nanocapillary
tip
filled
chitosan
(CS)/polyethylene
pyrrolidone
(PVP)
hydrogel
based
its
specific
pH-sensitive
properties.
The
gel-filled
has
potential
used
for
electrical
pH
detection
sensitivity
3.06
nA/pH
and
linear
range
from
7.3
4.3.
Such
measurement
extended
monitoring
(such
as
Escherichia
coli
Streptococcus
salivarius)
growth
because
relationship
between
growth.
Bacterial
curves
obtained
using
hydrogel-filled
showed
good
agreement
OD600
method.
Moreover,
this
device
applied
rapid
AST
tetracycline
norfloxacin
E.
minimum
inhibitory
concentrations
2
0.125
μg/mL,
respectively.
study
expands
hydrogel-based
research
by
changes
in
values.
Advanced Functional Materials,
Год журнала:
2024,
Номер
34(48)
Опубликована: Июнь 29, 2024
Abstract
While
existing
polyoxometalates
(POMs)
are
predominantly
utilized
in
a
range
of
energy
devices,
their
potential
bioelectronics
and
optoelectronics,
particularly
organic
photoelectrochemical
transistors
(OPECTs),
remains
unexplored.
Here,
the
POMs
this
aspect
is
unveiled
by
POM/sulfide‐gated
OPECT.
The
representative
PW
12
/ZnIn
2
S
4
with
cascading
light‐harvesting
enzyme‐like
functionality
enables
unique
on‐off
OPECT
operation.
incorporation
facilitates
separation
photogenerated
charge
carriers
across
heterojunction
interface,
consequently
inducing
pronounced
gating
effect.
Linking
CRISPR/Cas13a
system
correlating
target
miRNA‐21
biological
generation
H
O
,
will
enable
polymerization
dopamine
into
polydopamine
thus
sensitively
inhibit
gating.
Experimental
results
demonstrated
that
developed
sensor
achieved
good
analytical
performance
detection
limit
as
low
3.9
f
m
.
This
study
demonstrates
Given
large
family
multifunction
POMs,
its
implications
diverse
optoelectronics
expected.
Analytical Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 20, 2024
A
smartphone-mediated
self-powered
biosensor
is
fabricated
for
miRNA-141
detection
based
on
the
CRISPR/Cas12a
cross-cutting
technique
and
a
highly
efficient
nanozyme.
As
novel
nanozyme
signal-amplified
coreaction
accelerator,
AuPtPd@GDY
exhibits
an
excellent
ability
to
catalyze
cascade
color
reactions
high
conductivity
enhance
electrochemical
signal
assays.
After
of
S2-glucose
oxidase
(S2-GOD),
weakened,
detected
by
monitoring
decrease
in
signal.
On
other
hand,
reaction
among
glucose,
H
Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 4, 2024
Abstract
Solid‐state
nanochannel
biosensors
are
extensively
utilized
for
microRNA
(miRNA)
detection
owing
to
their
high
sensitivity
and
rapid
response.
However,
conventional
face
limitations
in
fixed
dynamic
range,
restricting
versatility
efficacy.
Herein,
we
introduce
tunable
triblock
DNA
probes
with
varying
affinities
target
miRNA
engineer
solid‐state
capable
of
customizable
range
adjustment.
The
architecture
comprises
a
poly‐adenine
(polyA)
block
adjustable
surface
density
anchoring,
alongside
stem
loop
blocks
modulating
structural
stability.
Through
systematic
manipulation
these
blocks,
demonstrate
the
ability
achieve
diverse
binding
limits,
achieving
an
initial
81‐fold
range.
By
combining
various
affinities,
extend
this
significantly
10,900‐fold.
Furthermore,
by
implementing
sequestration
mechanism,
effective
biosensor
is
narrowed
only
3‐fold
span
concentrations.
advanced
makes
them
highly
promising
broad
spectrum
biomedical
clinical
applications.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 2, 2025
Sensitive
and
direct
detection
of
circulating
microRNAs
(miRNAs)
in
undiluted
human
serums
can
significantly
facilitate
the
diagnosis
different
diseases
early
stages;
however,
achievement
such
a
goal
is
highly
challenged
by
extremely
complex
sample
matrix
nature
serums.
Here,
we
show
that
sensitive
rapid
let-7a
miRNA
biomarker
cancer
patient
be
realized
new
size-permeable
microcapsule
bioreactor.
The
Cas13a/crRNA-dsDNA@polyethylene
glycol
core/shell
biomicrocapsules
are
synthesized
simple
encapsulation
etching
steps.
Such
exhibit
high
stability,
enhanced
protein
antifouling
capability,
size-selective
permeability
only
allows
short
sequences
to
enter
core
cavities,
thereby
enabling
down
0.21
pM
within
20
min
via
signal
amplification
Cas13a/crRNA
near-infrared
(NIR)
fluorescence
signals.
biomicrocapsule
also
unambiguous
discrimination
clear
cell
renal
carcinoma
patients
from
healthy
groups
serum
samples,
highlighting
its
robust
clinical
potential
for
disease
diagnoses.
ACS Applied Materials & Interfaces,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 26, 2025
Early
cancer
detection
is
crucial
for
improving
patient
survival
rates.
However,
current
single-biomarker
methods
often
face
challenges,
such
as
insufficient
sensitivity,
poor
accuracy,
and
false
positives.
To
address
these
issues,
we
report
a
dual-functionalized
glass
micropipet
sensor
(DFMS)
capable
of
simultaneously
detecting
two
biomarkers,
nucleic
acids
proteins.
The
inner
surface
the
functionalized
with
amino-modified
silicon
nanowires
(SiNWs)
to
capture
disease-related
miRNAs,
enabling
ionic-current-based
detection,
while
outer
decorated
gold
nanoparticles
anchor
specific
protein
aptamers
Raman-based
detection.
This
dual-functionalization
significantly
enhances
sensitivity
selectivity
by
combining
ionic
amplification
plasmonic
Raman
signal
enhancement.
achieves
limits
1
aM
miRNAs
0.001
ng/mL
proteins,
minimal
mutual
interference
between
modes,
ensuring
accurate
independent
Validation
prostate
biomarkers
miRNA-1246
PSA,
well
gastric
miRNA-106a
CD44,
demonstrates
its
outstanding
selectivity,
stability,
broad
applicability,
providing
novel
approach
early
significant
clinical
implications.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 27, 2025
Clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
and
CRISPR-associated
(Cas)
protein
systems
are
renowned
for
their
high
sensitivity
specificity,
enabling
them
as
a
powerful
diagnostic
toolbox.
Multiplexed
detection
of
panels
targets,
opposed
to
single
is
imperative
reliable
conclusive
disease
diagnostics.
However,
multiplex
application
the
CRISPR/Cas
system
has
long
been
hindered
by
indistinguishable
signals
from
specific
targets
due
nonspecific
chaotic
trans-cleavage.
To
make
breakthrough,
substantial
efforts
have
devoted
CRISPR/Cas-powered
multiplexed
biosensing
strategies,
which
consequently
experienced
rapid
development
over
past
five
years.
This
review
systematically
summarizes
recent
advances
in
encompassing
Cas9,
Cas12,
Cas13.
Key
focus
issues
include
strategies
respective
advantages
limitations,
sensing
mechanisms,
performance
novel
validated
examples.
Finally,
status
challenges
critically
discussed,
future
outlooks
proposed
potential
practical
application.
Perspective
aims
inspire
significant
research
promote
next
generation
biosensing.
