Nano Letters,
Год журнала:
2024,
Номер
24(28), С. 8595 - 8601
Опубликована: Июнь 13, 2024
Protein
imaging
aids
diagnosis
and
drug
development
by
revealing
protein–drug
interactions
or
protein
levels.
However,
the
challenges
of
multiple
proteins,
reduced
sensitivity,
high
reliance
on
specific
properties
such
as
Raman
peaks
refractive
index
hinder
understanding.
Here,
we
introduce
multiprotein
colorful
through
signal
classification.
Our
method
utilized
machine
learning-assisted
classification
signals,
which
are
distinctive
features
label-free
proteins.
As
a
result,
three
types
proteins
could
be
imaged
simultaneously.
In
addition,
quantify
individual
from
mixture
over
wide
detection
range
(10
fg/mL–1
μg/mL).
These
results
showed
1000-fold
improvement
in
sensitivity
30-fold
increase
upper
limit
compared
to
existing
methods.
advances
will
enhance
our
understanding
biology
facilitate
disease
diagnoses
treatments.
ACS Applied Materials & Interfaces,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 24, 2025
The
year
2024
marks
the
50th
anniversary
of
discovery
surface-enhanced
Raman
spectroscopy
(SERS).
Over
recent
years,
SERS
has
experienced
rapid
development
and
became
a
critical
tool
in
biomedicine
with
its
unparalleled
sensitivity
molecular
specificity.
This
review
summarizes
advancements
challenges
substrates,
nanotags,
instrumentation,
spectral
analysis
for
biomedical
applications.
We
highlight
key
developments
colloidal
solid
an
emphasis
on
surface
chemistry,
hotspot
design,
3D
hydrogel
plasmonic
architectures.
Additionally,
we
introduce
innovations
including
those
interior
gaps,
orthogonal
reporters,
near-infrared-II-responsive
properties,
along
biomimetic
coatings.
Emerging
technologies
such
as
optical
tweezers,
nanopores,
wearable
sensors
have
expanded
capabilities
single-cell
single-molecule
analysis.
Advances
analysis,
signal
digitalization,
denoising,
deep
learning
algorithms,
improved
quantification
complex
biological
data.
Finally,
this
discusses
applications
nucleic
acid
detection,
protein
characterization,
metabolite
monitoring,
vivo
spectroscopy,
emphasizing
potential
liquid
biopsy,
metabolic
phenotyping,
extracellular
vesicle
diagnostics.
concludes
perspective
clinical
translation
SERS,
addressing
commercialization
potentials
tissue
sensing
imaging.
Journal of Neuroinflammation,
Год журнала:
2025,
Номер
22(1)
Опубликована: Фев. 7, 2025
Mitochondrial
dysfunction
is
a
pivotal
instigator
of
neuroinflammation,
with
mitochondrial
DNA
(mtDNA)
leakage
as
critical
intermediary.
This
review
delineates
the
intricate
pathways
leading
to
mtDNA
release,
which
include
membrane
permeabilization,
vesicular
trafficking,
disruption
homeostatic
regulation,
and
abnormalities
in
dynamics.
The
escaped
activates
cytosolic
sensors,
especially
cyclic
gmp-amp
synthase
(cGAS)
signalling
inflammasome,
initiating
neuroinflammatory
cascades
via
pathways,
exacerbating
spectrum
neurological
pathologies.
therapeutic
promise
targeting
discussed
detail,
underscoring
necessity
for
multifaceted
strategy
that
encompasses
preservation
homeostasis,
prevention
leakage,
reestablishment
dynamics,
inhibition
activation
sensors.
Advancing
our
understanding
complex
interplay
between
neuroinflammation
imperative
developing
precision
interventions
disorders.
ACS Nano,
Год журнала:
2024,
Номер
18(26), С. 17228 - 17239
Опубликована: Июнь 15, 2024
Nanoplastics
(NPs)
represent
a
growing
concern
for
global
environmental
health,
particularly
in
marine
ecosystems
where
they
predominantly
accumulate.
The
impact
of
NPs
on
benthic
organisms,
such
as
bivalves,
raises
critical
questions
regarding
ecological
integrity
and
food
safety.
Traditional
methods
assessing
NP
toxicity
are
often
limited
by
their
time-intensive
nature
ethical
considerations.
Herein,
we
explore
the
toxicological
effects
bivalve
Food Chemistry X,
Год журнала:
2024,
Номер
22, С. 101387 - 101387
Опубликована: Апрель 13, 2024
A
Se
site
targeted-two
circles
antioxidant
of
polyphenols
EGCG
and
genistein
in
glutathione
peroxidase
4
(GPx4)–like
catalytic
peroxide
H2O2
cumene
hydroperoxide
degradation
was
demonstrated
by
surface-enhanced
Raman
scattering
(SERS).
atom's
active
center
is
presenting
a
'low-oxidation'
'high-oxidation'
cycle.
The
former
oxidized
to
selenenic
acid
(SeO−)
with
bond
at
619/
610
cm−1
assigned
the
νO
−
substrate
544/
551
ωHSeC
decreased.
Under
oxidative
stress,
enzyme
shifted
cycle,
which
GPx4
shuttles
between
R-SeO−
R-SeOO−
intensity
840/
860
νOSe.
could
act
as
reducing
agent
both
Cu-OOH
degradation,
while,
can
only
reduce
Cu-OOH,
because
it
binds
more
readily
selenium
than
closer
proximity,
therefore
may
affect
its
simultaneous
binding
coenzymes.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 3, 2025
Inducing
programmed
cell
death
is
an
efficient
strategy
for
cancer
treatments,
and
a
deep
understanding
of
the
molecular
mechanisms
underlying
pathways
significance
rational
design
anticancer
drugs.
Herein,
propiolamide-mediated
crosstalk
between
ferroptosis
apoptosis
investigated.
In
situ
monitoring
reactive
oxygen
species
(ROS)
formation
structural
changes
propiolamide
compounds
achieved
by
Raman
spectroscopy.
The
propiolamides
in
inducing
monooxygenase-mediated
ROS
generation
inhibiting
GPX4
activities
are
revealed.
Furthermore,
pro-ferroptotic
pro-apoptotic
roles
containing
terminal
alkynes
verified.
This
study
provides
label-free
enzyme-drug
interactions
their
dynamics.
It
first
attempt
to
basis
through
two
important
enzymes
death.
paves
way
designing
novel
drugs
that
capable
triggering
synergistic
contribution
multiple
forms
efficacy.
Cell Biochemistry and Function,
Год журнала:
2025,
Номер
43(3)
Опубликована: Март 1, 2025
ABSTRACT
Cardiolipin
(CL),
an
exclusive
phospholipid,
is
predominantly
found
within
the
confines
of
inner
mitochondrial
membrane,
playing
indispensable
role
in
sustenance
operations
and
regulation
cellular
energy
metabolism.
The
influence
CL
on
pathways
cell
death
has
garnered
significant
interest
recent
scholarly
discourse.
This
review
delves
into
multifaceted
roles
across
various
modes
demise,
encompassing
apoptosis,
autophagy,
pyroptosis,
ferroptosis,
necrosis,
necroptosis.
discussion
extends
to
examination
CL's
implications
a
clinical
context,
particularly
concerning
cardiovascular
maladies,
neurological
degeneration,
oncological
conditions.
Through
integrative
analysis
contemporary
research
findings,
aim
elucidate
intricate
dynamics
involvement
phenomena.
While
acknowledging
inherent
limitations
hurdles
faced
by
current
endeavors,
therapeutic
potential
as
modulator
nonetheless
encouraging.
Forthcoming
investigations
must
surmount
these
obstacles,
thereby
uncovering
nuanced
mechanisms
impacts
realm
associated
pathologies,
potentially
paving
way
for
innovative
intervention
strategies.
Multi-biomarker
analysis
can
enhance
the
accuracy
of
single-biomarker
by
reducing
errors
caused
genetic
and
environmental
differences.
For
this
reason,
multi-biomarker
shows
higher
in
early
precision
diagnosis.
However,
conventional
methods
have
limitations
for
because
their
long
pre-processing
times,
inconsistent
results,
large
sample
requirements.
To
solve
these,
a
fast
accurate
diagnostic
method
is
introduced
lung
cancer
profiling
using
single
drop
blood.
this,
surface-enhanced
Raman
spectroscopic
immunoassay
(SERSIA)
employed
accurate,
quick,
reliable
quantification
biomarkers.
Then,
it
checked
statistical
relation
multi-biomarkers
to
differentiate
between
healthy
controls
patients.
This
approach
has
proven
effective;
with
20
µL
blood
serum,
diagnosed
92%
accuracy.
It
also
accurately
identifies
type
stage
87%
85%,
respectively.
These
results
show
importance
overcoming
challenges
posed
diagnostics.
Furthermore,
markedly
improves
multi-biomarker-based
methods,
illustrating
its
important
impact
on
clinical
