Polypyridiniums with Inherent Autophagy‐Inducing Activity for Atherosclerosis Treatment by Intracellularly Co‐Delivering Two Antioxidant Enzymes
Advanced Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 27, 2024
Abstract
Atherosclerosis
is
a
chronic
inflammatory
disease
of
the
arterial
intima
and
becoming
leading
cause
morbidity
mortality
worldwide.
There
considerable
evidence
that
defective
autophagy
overproduction
reactive
oxygen
species
(ROS)
are
closely
involved
in
development
progression
atherosclerosis.
Here,
polymer
developed
with
inherent
autophagy‐inducing
activity
to
treat
atherosclerosis
by
co‐delivering
antioxidant
enzymes.
The
lead
material
P5c
screened
from
library
polypyridiniums
shows
robust
efficacy
cytosolic
protein
delivery,
efficiently
delivers
superoxide
dismutase
(SOD)
catalase
(CAT)
into
macrophages
down‐regulate
intracellular
ROS.
Moreover,
activates
sufficiently
inhibits
foam
cell
formation.
nanoparticle
loaded
both
SOD
CAT
further
coated
neutrophil
membranes
an
ApoE
−/−
mice
model.
treatment
exhibits
potent
anti‐atherosclerosis
effect
via
activating
autophagy,
decreasing
infiltration
senescent
cells
atherosclerotic
plaques,
regulating
M2
polarization
macrophages,
restoring
structure
function
splenic
corpuscles.
offers
multifaceted
approach
combat
atherosclerosis,
addressing
cellular
dysfunction
need
for
targeted
delivery
within
affected
cells.
Язык: Английский
Rational Engineering of Cytosolic Delivery Systems for Protein Therapeutics
Accounts of Materials Research,
Год журнала:
2024,
Номер
5(10), С. 1194 - 1209
Опубликована: Сен. 10, 2024
Язык: Английский
A Dynamic Deferoxamine Polymer with Exceptional Performance in Mitochondrial Iron Depletion and Cytosolic Protein Delivery
Fang Zhu,
Lanfang Ren,
Wenhua Cheng
и другие.
Small,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 13, 2025
Abstract
Deferoxamine
(DFO)
is
an
FDA‐approved
naturally
occurring
iron
chelator
commonly
used
to
treat
transfusion‐induced
overload.
The
abundant
and
flexible
hydroxamic
acid
groups
in
DFO
enable
exceptional
binding
capacity
high
protein
via
hydrogen
bonding
interactions.
However,
the
applications
of
sequester
intracellular
deliver
proteins
inside
cells
are
limited
due
poor
membrane‐permeability.
Herein,
fabrication
a
dynamic
polymer
proposed
achieve
robust
delivery
efficient
mitochondrial
depletion.
Specifically,
grafted
onto
polycatechol
scaffold
catechol‐boronate
chemistry.
obtained
shows
capacity,
formed
complexes
show
resistance
toward
serum
proteins.
It
effectively
delivers
various
cargo
into
cytosol
treated
with
maintained
bioactivity.
In
addition,
cells,
released
efficiently
depletes
iron,
which
significantly
inhibits
oxidative
phosphorylation
glycolysis.
Remarkable
synergistic
cytotoxic
effects
achieved
when
loaded
toxic
This
study
provides
general
strategy
for
facile
preparation
bioactive
delivery,
designed
can
be
promising
carrier
therapeutics
cancer.
Язык: Английский
ROS-Responsive Cationic Polymers with Intrinsic Anti-Inflammatory Activity for Intracellular Protein Delivery
Yong-ming Wang,
Yangcan Ming,
Zhichao Yu
и другие.
Biomacromolecules,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 20, 2025
The
intracellular
delivery
of
protein
drugs
via
nanocarriers
offers
significant
potential
for
expanding
their
therapeutic
applications.
However,
the
unintended
activation
innate
immune
responses
and
inflammation
triggered
by
carriers
presents
a
major
challenge,
often
compromising
efficacy.
Here,
we
present
oligoethylenimine-thioketal
(OEI-TK),
reactive
oxygen
species-responsive
cationic
polymer
with
intrinsic
anti-inflammatory
properties,
to
overcome
this
challenge.
OEI-TK
self-assembles
electrostatically
bovine
serum
albumin
(BSA)
form
stable
nanoparticles
(OTB
NPs)
excellent
encapsulation
efficiency.
In
vitro
studies
confirmed
that
OTB
NPs
retained
OEI-TK's
antioxidant
enhanced
biocompatibility,
efficiently
delivered
BSA
into
cells.
Furthermore,
facilitated
β-galactosidase
while
preserving
its
enzymatic
activity,
demonstrating
functional
transport.
These
findings
highlight
as
promising
platform
dual
benefits
modulation
delivery,
holding
synergistic
treatment
inflammation-related
diseases.
Язык: Английский
Oxidative Stress, Glutaredoxins, and Their Therapeutic Potential in Posterior Capsular Opacification
Antioxidants,
Год журнала:
2024,
Номер
13(10), С. 1210 - 1210
Опубликована: Окт. 8, 2024
Posterior
capsular
opacification
(PCO)
is
the
most
common
long-term
complication
of
cataract
surgery.
Traditionally,
pathogenesis
PCO
involves
residual
lens
epithelial
cells
(LECs),
which
undergo
transdifferentiation
into
a
myofibroblast
phenotype,
hyperproliferation,
matrix
contraction,
and
deposition.
This
process
driven
by
marked
upregulation
inflammatory
growth
factors
post-surgery.
Recently,
research
on
role
redox
environments
has
gained
considerable
attention.
LECs,
are
in
direct
contact
with
aqueous
humour
after
surgery,
subjected
to
oxidative
stress
due
decreased
levels
reduced
glutathione
increased
oxygen
content
compared
outer
fibre
layer
before
In
this
review,
we
examine
critical
formation.
We
also
focus
glutaredoxins
(Grxs),
antioxidative
enzymes
produced
via
deglutathionylation,
their
protective
against
formation,
therapeutic
potential.
Furthermore,
discuss
latest
advancements
therapy,
particularly
development
advanced
pharmacological
agents,
emphasise
importance
approaches
anti-inflammatory
antioxidant
treatments
management.
conclusion,
review
highlights
significant
roles
PCO,
effects
Grxs
potential
therapies
treating
PCO.
Язык: Английский
Nanocarriers for intracellular delivery of proteins in biomedical applications: strategies and recent advances
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Ноя. 10, 2024
Protein
drugs
are
of
great
importance
in
maintaining
the
normal
functioning
living
organisms.
Indeed,
they
have
been
instrumental
combating
tumors
and
genetic
diseases
for
decades.
Among
these
pharmaceutical
agents,
those
that
target
intracellular
components
necessitate
use
therapeutic
proteins
to
exert
their
effects
within
targeted
cells.
However,
protein
is
limited
by
short
half-life
potential
adverse
physiological
environment.
The
advent
nanoparticles
offers
a
promising
avenue
prolonging
drugs.
This
achieved
encapsulating
proteins,
thereby
safeguarding
biological
activity
ensuring
precise
delivery
into
nanomaterial-based
drug
system
mitigates
rapid
hydrolysis
unwarranted
diffusion
minimizing
side
circumventing
limitations
inherent
traditional
techniques
like
electroporation.
review
examines
established
systems,
including
based
on
polymers,
liposomes,
nanoparticles.
We
delve
operational
principles
transport
mechanisms
nanocarriers,
discussing
various
considerations
essential
designing
cutting-edge
platforms.
Additionally,
we
investigate
innovative
designs
applications
cytosolic
systems
medical
research
clinical
practice,
particularly
areas
tumor
treatment,
gene
editing
fluorescence
imaging.
sheds
light
current
restrictions
anticipates
future
avenues,
aiming
foster
continued
advancement
this
field.
Язык: Английский
A Hydroxyquinoline Polymer with Excellent Amyloidosis Inhibition and Protein Delivery Ability to Combat Amyloid-β-Mediated Neurotoxicity
Nano Letters,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 1, 2024
The
accumulation
of
abnormal
protein
deposits
known
as
amyloid-β
(Aβ)
plaques
contributes
to
the
development
and
progression
Alzheimer's
disease.
Aggregated
Aβ
exacerbates
oxidative
stress
by
stimulating
production
reactive
oxygen
species
(ROS)
in
a
detrimental
feedback
loop.
8-Hydroxyquinoline
(8-HQ)
is
recognized
for
its
ability
inhibit
or
reverse
aggregation
reduce
neurotoxicity.
Here,
an
8-HQ-based
polymer,
DHQ,
was
developed
combat
Aβ-mediated
neurotoxicity
delivering
antioxidant
enzyme.
DHQ
efficiently
delivers
superoxide
dismutase
into
targeted
cells,
thereby
downregulating
intracellular
ROS
level.
Additionally,
polymer
effectively
inhibits
fibrillization
three
proteins
involved
fibrosis,
β-lactoglobulin
(BLG),
insulin,
Aβ1-40,
at
nanomolar
concentrations.
Cell
culture
models
demonstrated
that
reduces
levels
induced
Aβ1-40
aggregation,
rescuing
cell
viability
preventing
apoptosis.
Intracellular
delivery
SOD
further
enhanced
maintain
homeostasis.
This
offers
multifaceted
approach
treating
diseases
associated
with
amyloidosis.
Язык: Английский
Zwitterionic polymers with high serum tolerance for intracellular protein delivery
Biomaterials Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Modification
with
benzenesulphonate
on
a
phase-transition
polymer
can
improve
the
protein
delivery
efficacy,
reduce
cytotoxicity
and
adsorption.
Язык: Английский
A Supramolecular Deferoxamine–Crisaborole Nanoparticle Targets Ferroptosis, Inflammation, and Oxidative Stress in the Treatment of Retinal Ischemia/Reperfusion Injury
Nano Letters,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 13, 2024
Retinal
ischemia-reperfusion
(IR)
injury
is
a
major
cause
of
vision
loss
worldwide,
with
ferroptosis,
oxidative
stress,
and
inflammation
playing
key
roles
in
its
pathogenesis.
Currently,
treatments
targeting
multiple
aspects
this
condition
are
limited.
This
study
introduces
supramolecular
nanoparticle
combining
the
phosphodiesterase
4
(PDE4)
inhibitor
crisaborole
ferroptosis
deferoxamine
to
address
these
pathological
processes.
Crisaborole
forms
dynamic
bond
via
benzoxaborole-catechol
chemistry,
creating
an
amphiphilic
molecule
that
assembles
into
nanoparticles.
Treatment
nanoparticles
enhances
glutathione
peroxidase
(GPX4)
levels,
downregulates
ferroptosis-related
genes
[Acyl-CoA
synthetase
long
chain
family
member
(
Язык: Английский