In vivo 2H‐MR spectroscopy and imaging of hepatic metabolic formation of trimethylamine‐N‐oxide DOI Creative Commons

Hadar Dessau,

Talia Harris, Robin A. de Graaf

и другие.

Magnetic Resonance in Medicine, Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

Abstract Purpose Despite growing evidence of the link between elevated levels trimethylamine‐N‐oxide (TMAO) and multiple diseases, there is no method with which to spatially monitor its hepatic formation from interstitially produced trimethylamine (TMA). This study aimed develop a deuterium metabolic spectroscopy (DMS) imaging (DMI) approach detect TMA‐to‐TMAO metabolism in vivo. Methods The 2 H 9 ‐TMA (TMA‐ d ) ‐TMAO (TMAO‐ cells overexpressing enzyme flavin‐dependent monooxygenase 3 (FMO3) was monitored vitro H‐NMR. Using an ultrahigh‐field (15.2T) MRI scanner, orally administered TMA‐ TMAO‐ studied mice DMS DMI. Results spectrally resolved H‐NMR peaks intracellularly (3.1 ppm) that supplemental (2.7 could be detected only overexpressed FMO3. In vivo, DMI experiments performed after oral administration revealed conversion high liver females, express contrast, indication production males, agreement reports role testosterone downregulating expression Conclusion work shows ability use H‐MR‐based methodologies pathway thus should explored further investigate TMAO diverse pathologies.

Язык: Английский

In vivo 2H‐MR spectroscopy and imaging of hepatic metabolic formation of trimethylamine‐N‐oxide DOI Creative Commons

Hadar Dessau,

Talia Harris, Robin A. de Graaf

и другие.

Magnetic Resonance in Medicine, Год журнала: 2025, Номер unknown

Опубликована: Апрель 14, 2025

Abstract Purpose Despite growing evidence of the link between elevated levels trimethylamine‐N‐oxide (TMAO) and multiple diseases, there is no method with which to spatially monitor its hepatic formation from interstitially produced trimethylamine (TMA). This study aimed develop a deuterium metabolic spectroscopy (DMS) imaging (DMI) approach detect TMA‐to‐TMAO metabolism in vivo. Methods The 2 H 9 ‐TMA (TMA‐ d ) ‐TMAO (TMAO‐ cells overexpressing enzyme flavin‐dependent monooxygenase 3 (FMO3) was monitored vitro H‐NMR. Using an ultrahigh‐field (15.2T) MRI scanner, orally administered TMA‐ TMAO‐ studied mice DMS DMI. Results spectrally resolved H‐NMR peaks intracellularly (3.1 ppm) that supplemental (2.7 could be detected only overexpressed FMO3. In vivo, DMI experiments performed after oral administration revealed conversion high liver females, express contrast, indication production males, agreement reports role testosterone downregulating expression Conclusion work shows ability use H‐MR‐based methodologies pathway thus should explored further investigate TMAO diverse pathologies.

Язык: Английский

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