A Practical Spectroscopic Protocol Based on Sialic Acid‐S‐Protein Interaction for SARS‐CoV‐2 Detection DOI
Vasfiye Hazal Özyurt, Burak Ekrem Çitil, Ülkü Anık

и другие.

ChemistrySelect, Год журнала: 2024, Номер 9(47)

Опубликована: Дек. 1, 2024

Abstract This work aims to investigate the possible role of sialic acids as host cell receptors for SARS‐CoV‐2 infection and hence develop a practical spectroscopic protocol that relies on interaction between fetuin spike protein (S‐protein) detection. For purpose, firstly, synthesized gold nanoparticles (AuNPs) were functionalized with fetuin. Then, AuNPs incubated S‐protein difference in absorbance value at 530 nm was measured spectroscopically. The characterized by using fourier transform infrared spectroscopy atomic force microscopy methods whereas sole AuNP structure investigated transmission electron technique. experimental parameters such amount (0, 5, 10, 20 µg/mL) incubation duration 20, 30, 40 min) optimized analytical characteristics developed biosensors investigated. detection limit calculated 2.40 ng/mL, linear range 4.98 95.2 relative standard deviation 0.72% 9.95 ng/mL S‐protein. Finally, biosensor applied real samples taken from non‐infected infected individuals.

Язык: Английский

Alzheimer’s, Parkinson’s, Frontotemporal Lobar Degeneration, and Amyotrophic Lateral Sclerosis Start in Pediatric Ages: Ultrafine Particulate Matter and Industrial Nanoparticles Are Key in the Early-Onset Neurodegeneration: Time to Invest in Preventive Medicine DOI Creative Commons
Lilian Calderón‐Garcidueñas, Angélica González-Maciel, Rafael Reynoso-Robles

и другие.

Toxics, Год журнала: 2025, Номер 13(3), С. 178 - 178

Опубликована: Фев. 28, 2025

Billions of people are exposed to fine particulate matter (PM2.5) levels above the USEPA’s annual standard 9 μg/m3. Common emission sources anthropogenic, producing complex aerosolized toxins. Ultrafine (UFPM) and industrial nanoparticles (NPs) have major detrimental effects on brain, but USA does not measure UFPM a routine basis. This review focuses development progression common neurodegenerative diseases, as diagnosed through neuropathology, among young residents in Metropolitan Mexico City (MMC). MMC is one most polluted megacities world, with population 22 million residents, many whom unaware brain caused by their atmosphere. Fatal diseases (such Alzheimer’s Parkinson’s) that begin childhood populations living air environments preventable. We conclude UFPM/NPs capable disrupting neural homeostasis give rise relentless processes throughout entire life highly MMC. The paradigm reaching old age neurodegeneration no longer supported. Neurodegenerative changes start early pediatric ages irreversible. It time invest preventive medicine.

Язык: Английский

Процитировано

0

A Practical Spectroscopic Protocol Based on Sialic Acid‐S‐Protein Interaction for SARS‐CoV‐2 Detection DOI
Vasfiye Hazal Özyurt, Burak Ekrem Çitil, Ülkü Anık

и другие.

ChemistrySelect, Год журнала: 2024, Номер 9(47)

Опубликована: Дек. 1, 2024

Abstract This work aims to investigate the possible role of sialic acids as host cell receptors for SARS‐CoV‐2 infection and hence develop a practical spectroscopic protocol that relies on interaction between fetuin spike protein (S‐protein) detection. For purpose, firstly, synthesized gold nanoparticles (AuNPs) were functionalized with fetuin. Then, AuNPs incubated S‐protein difference in absorbance value at 530 nm was measured spectroscopically. The characterized by using fourier transform infrared spectroscopy atomic force microscopy methods whereas sole AuNP structure investigated transmission electron technique. experimental parameters such amount (0, 5, 10, 20 µg/mL) incubation duration 20, 30, 40 min) optimized analytical characteristics developed biosensors investigated. detection limit calculated 2.40 ng/mL, linear range 4.98 95.2 relative standard deviation 0.72% 9.95 ng/mL S‐protein. Finally, biosensor applied real samples taken from non‐infected infected individuals.

Язык: Английский

Процитировано

0