Nanoconfinement Effect and Nanozyme Catalysis Enhance ILu/HOF-14 Electrochemiluminescence for Biosensing DOI
Qianqian Cai, Yuehui Wang,

Guifen Jie

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 12, 2025

A low collision frequency and an insufficient number of free radicals are the main problems leading to electroluminescence (ECL) efficiency luminol its derivatives. In order solve above issues, this work used nanoconfinement combined with nanozyme catalysis significantly enhance ECL efficiency. We assembled isoluminol (ILu) into hydrogen-bonded organic framework HOF-14 prepared a novel emitter ILu/HOF-14 for first time. Surprisingly, compared ILu/H2O2 system, signal ILu/HOF-14/H2O2 was increased by 33 times. This because porous structure effectively limited movement their frequency. Therefore, reaction rate between improved achieve amplification. To further increase radicals, we introduced hybrid Zn SAC@CuO2 NPs superior peroxidase (POD)-like activity. It could catalyze coreactant H2O2 produce large amount ROS (OH• O2•-), accelerating ILu improving signal. Based on research, dual-mode biosensing imaging platform constructed detect microcystin-LR (MC-LR). nonspecific trans-cleavage activity CRISPR-Cas12a system dynamic continuity amplification capability platform, detection sensitivity broadening avenues molecular diagnostic strategies.

Язык: Английский

Nanoconfinement Effect and Nanozyme Catalysis Enhance ILu/HOF-14 Electrochemiluminescence for Biosensing DOI
Qianqian Cai, Yuehui Wang,

Guifen Jie

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 12, 2025

A low collision frequency and an insufficient number of free radicals are the main problems leading to electroluminescence (ECL) efficiency luminol its derivatives. In order solve above issues, this work used nanoconfinement combined with nanozyme catalysis significantly enhance ECL efficiency. We assembled isoluminol (ILu) into hydrogen-bonded organic framework HOF-14 prepared a novel emitter ILu/HOF-14 for first time. Surprisingly, compared ILu/H2O2 system, signal ILu/HOF-14/H2O2 was increased by 33 times. This because porous structure effectively limited movement their frequency. Therefore, reaction rate between improved achieve amplification. To further increase radicals, we introduced hybrid Zn SAC@CuO2 NPs superior peroxidase (POD)-like activity. It could catalyze coreactant H2O2 produce large amount ROS (OH• O2•-), accelerating ILu improving signal. Based on research, dual-mode biosensing imaging platform constructed detect microcystin-LR (MC-LR). nonspecific trans-cleavage activity CRISPR-Cas12a system dynamic continuity amplification capability platform, detection sensitivity broadening avenues molecular diagnostic strategies.

Язык: Английский

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