Mirror-image protein and peptide drug discovery through mirror-image phage display
Chem,
Год журнала:
2024,
Номер
10(8), С. 2390 - 2407
Опубликована: Июль 5, 2024
Язык: Английский
Sortases: structure, mechanism, and implications for protein engineering
Trends in Biochemical Sciences,
Год журнала:
2024,
Номер
49(7), С. 596 - 610
Опубликована: Апрель 30, 2024
Язык: Английский
Sortase-mediated labeling: Expanding frontiers in site-specific protein functionalization opens new research avenues
Current Opinion in Chemical Biology,
Год журнала:
2024,
Номер
80, С. 102443 - 102443
Опубликована: Март 19, 2024
Язык: Английский
Empowering Site‐Specific Bioconjugations In Vitro and In Vivo: Advances in Sortase Engineering and Sortase‐Mediated Ligation
Angewandte Chemie International Edition,
Год журнала:
2023,
Номер
63(12)
Опубликована: Дек. 12, 2023
Sortase-mediated
ligation
(SML)
has
emerged
as
a
powerful
and
versatile
methodology
for
site-specific
protein
conjugation,
functionalization/labeling,
immobilization,
design
of
biohybrid
molecules
systems.
However,
the
broader
application
SML
faces
several
challenges,
such
limited
activity
stability,
dependence
on
calcium
ions,
reversible
reactions
caused
by
nucleophilic
side-products.
Over
past
decade,
engineering
campaigns
particularly
directed
evolution,
have
been
extensively
employed
to
overcome
sortase
limitations,
thereby
expanding
potential
in
multiple
directions,
including
therapeutics,
biorthogonal
chemistry,
biomaterials,
biosensors.
This
review
provides
an
overview
achieved
advancements
highlights
recent
progress
utilizing
combination
with
other
state-of-the-art
chemical
biological
methodologies.
The
aim
is
encourage
scientists
employ
sortases
their
conjugation
experiments.
Язык: Английский
Efficient chemical synthesis of mirror-image DNA polymerase Dpo4 assisted by one-pot multi-segment condensation
Organic & Biomolecular Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
This
study
describes
an
efficient
chemical
synthesis
method
of
d
-Dpo4,
a
valuable
enzymatic
tool
producing
mirror-image
DNAs,
using
one-pot
multi-segment
condensation
strategy.
Язык: Английский
Development and applications of enzymatic peptide and protein ligation
Journal of Peptide Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 21, 2024
Chemical
synthesis
of
complex
peptides
and
proteins
continues
to
play
increasingly
important
roles
in
industry
academia,
where
strategies
for
covalent
ligation
two
or
more
peptide
fragments
produce
longer
convergent
manners
have
become
critical.
In
recent
decades,
efficient
site‐selective
mediated
by
exploiting
the
biocatalytic
capacity
nature's
diverse
toolkit
(i.e.,
enzymes)
been
widely
recognized
as
a
powerful
extension
existing
chemical
strategies.
this
review,
we
present
chronological
overview
development
proteases,
transpeptidases,
transglutaminases,
ubiquitin
ligases.
We
survey
different
properties
between
reactions
various
enzymes,
including
selectivity
efficiency
reaction,
“scar”
left
product,
type
amide
bond
formed
(natural
isopeptide),
synthetic
availability
reactants,
whether
enzymes
are
orthogonal
another.
This
review
also
describes
how
inherent
specificity
these
can
be
exploited
protein
ligation.
Язык: Английский
Generating a mirror-image monobody targeting MCP-1 via TRAP display and chemical protein synthesis
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Дек. 23, 2024
Biologically
produced
protein
drugs
are
generally
susceptible
to
degradation
by
proteases
and
often
exhibit
immunogenicity.
To
address
this
issue,
mirror-image
peptide/protein
binders
consisting
of
D-amino
acids
have
been
developed
so
far
through
the
phage
display
technique.
Here,
we
develop
a
binder
derived
from
monobody,
one
promising
scaffolds,
utilizing
two
notable
technologies:
chemical
synthesis
TRAP
display,
an
improved
version
mRNA
display.
A
sequential
workflow
initial
screening
followed
affinity
maturation,
facilitated
generates
L-monobody
with
high
(KD
=
1.3
nM)
against
monocyte
chemoattractant
protein-1
(MCP-1)
D-enantiomer.
The
chemically
synthesized
D-monobody
demonstrates
strong
specific
binding
L-MCP-1
exhibits
pharmaceutically
favorable
properties
such
as
proteolytic
resistance,
minimal
immune
response,
potent
inhibitory
effect
on
MCP-1-induced
cell
migration.
This
study
elevates
value
alternative
modality
in
drug
discovery.
In
work,
monobody
targeting
MCP-1
is
generated
via
vitro
technology
synthesis.
It
properties,
including
protease
negligible
immunogenicity,
Язык: Английский
Empowering Site‐Specific Bioconjugations In Vitro and In Vivo: Advances in Sortase Engineering and Sortase‐Mediated Ligation
Angewandte Chemie,
Год журнала:
2023,
Номер
136(12)
Опубликована: Дек. 12, 2023
Abstract
Sortase‐mediated
ligation
(SML)
has
emerged
as
a
powerful
and
versatile
methodology
for
site‐specific
protein
conjugation,
functionalization/labeling,
immobilization,
design
of
biohybrid
molecules
systems.
However,
the
broader
application
SML
faces
several
challenges,
such
limited
activity
stability,
dependence
on
calcium
ions,
reversible
reactions
caused
by
nucleophilic
side‐products.
Over
past
decade,
engineering
campaigns
particularly
directed
evolution,
have
been
extensively
employed
to
overcome
sortase
limitations,
thereby
expanding
potential
in
multiple
directions,
including
therapeutics,
biorthogonal
chemistry,
biomaterials,
biosensors.
This
review
provides
an
overview
achieved
advancements
highlights
recent
progress
utilizing
combination
with
other
state‐of‐the‐art
chemical
biological
methodologies.
The
aim
is
encourage
scientists
employ
sortases
their
conjugation
experiments.
Язык: Английский