Directed evolution of Escherichia coli surface-displayed Vitreoscilla hemoglobin as an artificial metalloenzyme for the synthesis of 5-imino-1,2,4-thiadiazoles

Chemical Science, Год журнала: 2024, Номер 15(20), С. 7742 - 7748

Опубликована: Янв. 1, 2024

Escherichia coli surface-displayed Vitreoscilla hemoglobin as an artificial metalloenzyme for the synthesis of 5-imino-1,2,4-thiadiazoles.

Язык: Английский

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Construction of Chiral Tricyclic Structures Using Whole‐Cell Biocatalysts: A Strategy via the Cyclopropanation of Unsaturated π‐Systems

Chinese Journal of Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 28, 2025

Comprehensive Summary Chiral tricyclic compounds represent a significant class of biologically active substances with wide‐ranging applications in materials science, medicinal chemistry, as well the food and fragrance industries. Here, we combine CAST (combinatorial site saturation test) bacterial surface display technology to develop laboratory‐evolved whole‐cell catalyst, designated SD‐VHb Tric (surface vitreoscilla hemoglobin). This novel carbene‐transferase is based on engineered VHb employs strategy for cyclopropanation unsaturated π‐systems synthesize various chiral structures. exhibits unparalleled stereocontrol (up 99.9% de 99% ee ) good reactivity, enabling synthesis structures diverse aromatic or heterocyclic ring scaffolds from simple starting materials. Additionally, computational studies were conducted elucidate crucial role intermolecular hydrophobic interactions regulating reaction, while also demonstrating impact adaptive changes pocket size orientation ligand.

Язык: Английский

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Visible-light-induced [3+3] cycloaddition reaction of phenol and hydrazone to access 1,3,4-oxadiazines scaffolds

Molecular Catalysis, Год журнала: 2024, Номер 561, С. 114156 - 114156

Опубликована: Апрель 23, 2024

Язык: Английский

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Switching engineered Vitreoscilla hemoglobin into carbene transferase for enantioselective S H insertion

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 278, С. 134756 - 134756

Опубликована: Авг. 13, 2024

Язык: Английский

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A Green Synthesis of 3-Selanyl-Isoflavones via Lipase Mediated Selenylation/Cyclization of Enaminones

Catalysts, Год журнала: 2024, Номер 14(7), С. 413 - 413

Опубликована: Июнь 28, 2024

Herein, a green biocatalytic approach using lipase as catalyst has been developed for the synthesis of 3-selanyl-isoflavones through selenylation/cyclization 2-hydroxyphenyl enaminones and diphenyl di-selenide under mild conditions. The environmentally friendly method reached high yields 87–95% in short time at 30 °C, with 17 examples successfully prepared. Furthermore, we have investigated possible mechanisms underlying this reaction.

Язык: Английский

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Visible-Light-Driven Synthesis of N-Alkyl α-Amino Acid Derivatives from Unactivated Alkyl Bromides and In Situ Generated Imines

Organic Letters, Год журнала: 2024, Номер unknown

Опубликована: Сен. 23, 2024

One-pot, multicomponent reactions are known for their green and efficient nature. We report a novel three-component reaction of alkyl amines, glyoxylates, unactivated bromides under visible-light-induced palladium catalysis, yielding

Язык: Английский

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Hemoglobin: An efficient oxidase for the green synthesis of quinazoline derivatives by biocatalytic domino strategy

Molecular Catalysis, Год журнала: 2024, Номер 570, С. 114680 - 114680

Опубликована: Ноя. 21, 2024

Язык: Английский

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Enzymatic and convenient synthesis of polyhydroxyalkyl pyrroles from unprotected sugars, benzoylacetonitriles, and NH4OAc/Benzylamines in water

Molecular Catalysis, Год журнала: 2024, Номер 572, С. 114725 - 114725

Опубликована: Дек. 1, 2024

Язык: Английский

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