Challenges and opportunities in micro/nanofluidic and lab-on-a-chip

Progress in molecular biology and translational science, Год журнала: 2022, Номер unknown, С. 289 - 302

Опубликована: Янв. 1, 2022

Язык: Английский

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Human mini-blood–brain barrier models for biomedical neuroscience research: a review

Biomaterials Research, Год журнала: 2022, Номер 26(1)

Опубликована: Сен. 30, 2022

The human blood-brain barrier (BBB) is a unique multicellular structure that in critical demand for fundamental neuroscience studies and therapeutic evaluation. Despite substantial achievements creating vitro BBB platforms, challenges generating specifics of physiopathological relevance are viewed as impediments to the establishment models. In this review, we provide insight into development deployment models allow investigation physiology pathology neurological avenues. First, highlight components, including cell sources, biomaterial glue collections, engineering techniques reconstruct miniaturized BBB. Second, describe recent breakthroughs mini-BBBs investigating biological mechanisms neurology. Finally, discuss application medical approaches. This review provides strategies understanding diseases, validation model drug discovery, potential approach personalized medicine.

Язык: Английский

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Porous Polymeric Nanofilms for Recreating the Basement Membrane in an Endothelial Barrier-on-Chip

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(10), С. 13006 - 13017

Опубликована: Фев. 28, 2024

Organs-on-chips (OoCs) support an organotypic human cell culture in vitro. Precise representation of basement membranes (BMs) is critical for mimicking physiological functions tissue interfaces. Artificial polyester (PES) and polycarbonate (PC) commonly used vitro models OoCs do not replicate the characteristics natural BMs, such as submicrometric thickness, selective permeability, elasticity. This study introduces porous poly(d,l-lactic acid) (PDLLA) nanofilms replicating BMs demonstrates their integration into microfluidic chips. Using roll-to-roll gravure coating polymer phase separation, we fabricated transparent ∼200 nm thick PDLLA films. These are 60 times thinner 27 more elastic than PES show uniformly distributed pores controlled diameter (0.4 to 1.6 μm), which favor compartmentalization exchange large water-soluble molecules. Human umbilical vein endothelial cells (HUVECs) on stretched across microchannels exhibited 97% viability, enhanced adhesion, a higher proliferation rate compared performance glass substrates. After 5 days culture, HUVECs formed functional barrier suspended nanofilms, confirmed by 10-fold increase transendothelial electrical resistance blocked 150 kDa dextran diffusion. When integrated between two channels exposed shear stress, despite ultrathin upheld integrity efficiently maintained separation channels. The successful formation adherent endothelium coculture astrocytes either side nanofilm validate it artificial BM OoCs. Its thickness guarantees intimate contact, key feature mimic blood–brain paracrine signaling types. In summary, hold potential improving accuracy relevance OoC drug discovery tools.

Язык: Английский

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The Modular µSiM Reconfigured: Integration of Microfluidic Capabilities to Study In Vitro Barrier Tissue Models under Flow

Advanced Healthcare Materials, Год журнала: 2022, Номер 11(21)

Опубликована: Авг. 12, 2022

Microfluidic tissue barrier models have emerged to address the lack of physiological fluid flow in conventional "open-well" Transwell-like devices. However, microfluidic techniques not achieved widespread usage bioscience laboratories because they are fully compatible with traditional experimental protocols. To advance research, there is a need for platform that combines key advantages both open-well and systems. Here, plug-and-play module developed introduce on-demand capabilities an device features nanoporous membrane live-cell imaging capabilities. The magnetic latching assembly this design enables bi-directional reconfiguration allows users conduct experiment format established protocols then add or remove as desired. This work also provides experimentally-validated model select conditions based on needs. As proof-of-concept, flow-induced alignment endothelial cells expression shear-sensitive gene targets demonstrated, different phases neutrophil transmigration across chemically stimulated monolayer under visualized. With these capabilities, it anticipated engineering will adopt reconfigurable due compatibility standard

Язык: Английский

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Hybrid Microfluidic Chip Design with Two‐Photon Polymerized Protein‐Based Hydrogel Microstructures for Single Cell Experiments

Advanced Materials Technologies, Год журнала: 2025, Номер unknown

Опубликована: Янв. 10, 2025

Abstract Although hydrogels are among the most promising materials for a huge variety of biomimicking and tissue engineering applications, conventional such as polydimethylsiloxane (PDMS) still outweigh in terms processability production microfluidic devices. Hence, incorporating hydrogel components inside PDMS‐based chips is approach to take advantage many possibilities utilize hydrogels, while maintaining standard properties devices mechanical stability. Microfluidic produced by soft lithography combined with high‐resolution protein‐based elements fabricated two‐photon polymerization (2PP). Those hybrid used distinguish different cell phenotypes injecting pancreatic cancer cells device investigate interactions microstructures. The Young's modulus blocks printed at experimental conditions determined atomic force microscopy measurements. To showcase high 3D resolution presented fabrication method, fully fibrous meshes configurations microchannels. By measuring velocity circularity that pass through varying densities, impact on flow determined. Furthermore, precursor solution successfully removed immersed phosphate buffered saline.

Язык: Английский

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