The Modular µSiM Reconfigured: Integration of Microfluidic Capabilities to Study In Vitro Barrier Tissue Models under Flow DOI
Mehran Mansouri, Adeel Ahmed, S. Danial Ahmad

и другие.

Advanced Healthcare Materials, Год журнала: 2022, Номер 11(21)

Опубликована: Авг. 12, 2022

Microfluidic tissue barrier models have emerged to address the lack of physiological fluid flow in conventional "open-well" Transwell-like devices. However, microfluidic techniques not achieved widespread usage bioscience laboratories because they are fully compatible with traditional experimental protocols. To advance research, there is a need for platform that combines key advantages both open-well and systems. Here, plug-and-play module developed introduce on-demand capabilities an device features nanoporous membrane live-cell imaging capabilities. The magnetic latching assembly this design enables bi-directional reconfiguration allows users conduct experiment format established protocols then add or remove as desired. This work also provides experimentally-validated model select conditions based on needs. As proof-of-concept, flow-induced alignment endothelial cells expression shear-sensitive gene targets demonstrated, different phases neutrophil transmigration across chemically stimulated monolayer under visualized. With these capabilities, it anticipated engineering will adopt reconfigurable due compatibility standard

Язык: Английский

Hydrogel-Encapsulated Pancreatic Islet Cells as a Promising Strategy for Diabetic Cell Therapy DOI Creative Commons
Zhikun Huan, Jingbo Li, Zhiqiang Luo

и другие.

Research, Год журнала: 2024, Номер 7

Опубликована: Янв. 1, 2024

Islet transplantation has now become a promising treatment for insulin-deficient diabetes mellitus. Compared to traditional treatments, cell therapy can restore endogenous insulin supplementation, but its large-scale clinical application is impeded by donor shortages, immune rejection, and unsuitable sites. To overcome these challenges, an increasing number of studies have attempted transplant hydrogel-encapsulated islet cells treat diabetes. This review mainly focuses on the strategy pancreatic diabetic therapy, including different sources encapsulated in hydrogels, encapsulation methods, hydrogel types, series accessorial manners improve outcomes. In addition, formation challenges as well prospects are also presented.

Язык: Английский

Процитировано

5

Challenges and opportunities in micro/nanofluidic and lab-on-a-chip DOI
Nidhi Verma, Alok Pandya

Progress in molecular biology and translational science, Год журнала: 2022, Номер unknown, С. 289 - 302

Опубликована: Янв. 1, 2022

Язык: Английский

Процитировано

17

Human mini-blood–brain barrier models for biomedical neuroscience research: a review DOI Creative Commons
Minh Tran, Chaejeong Heo, Luke P. Lee

и другие.

Biomaterials Research, Год журнала: 2022, Номер 26(1)

Опубликована: Сен. 30, 2022

The human blood-brain barrier (BBB) is a unique multicellular structure that in critical demand for fundamental neuroscience studies and therapeutic evaluation. Despite substantial achievements creating vitro BBB platforms, challenges generating specifics of physiopathological relevance are viewed as impediments to the establishment models. In this review, we provide insight into development deployment models allow investigation physiology pathology neurological avenues. First, highlight components, including cell sources, biomaterial glue collections, engineering techniques reconstruct miniaturized BBB. Second, describe recent breakthroughs mini-BBBs investigating biological mechanisms neurology. Finally, discuss application medical approaches. This review provides strategies understanding diseases, validation model drug discovery, potential approach personalized medicine.

Язык: Английский

Процитировано

17

Porous Polymeric Nanofilms for Recreating the Basement Membrane in an Endothelial Barrier-on-Chip DOI Creative Commons
Elena Mancinelli,

Nanami Zushi,

Megumi Takuma

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(10), С. 13006 - 13017

Опубликована: Фев. 28, 2024

Organs-on-chips (OoCs) support an organotypic human cell culture in vitro. Precise representation of basement membranes (BMs) is critical for mimicking physiological functions tissue interfaces. Artificial polyester (PES) and polycarbonate (PC) commonly used vitro models OoCs do not replicate the characteristics natural BMs, such as submicrometric thickness, selective permeability, elasticity. This study introduces porous poly(d,l-lactic acid) (PDLLA) nanofilms replicating BMs demonstrates their integration into microfluidic chips. Using roll-to-roll gravure coating polymer phase separation, we fabricated transparent ∼200 nm thick PDLLA films. These are 60 times thinner 27 more elastic than PES show uniformly distributed pores controlled diameter (0.4 to 1.6 μm), which favor compartmentalization exchange large water-soluble molecules. Human umbilical vein endothelial cells (HUVECs) on stretched across microchannels exhibited 97% viability, enhanced adhesion, a higher proliferation rate compared performance glass substrates. After 5 days culture, HUVECs formed functional barrier suspended nanofilms, confirmed by 10-fold increase transendothelial electrical resistance blocked 150 kDa dextran diffusion. When integrated between two channels exposed shear stress, despite ultrathin upheld integrity efficiently maintained separation channels. The successful formation adherent endothelium coculture astrocytes either side nanofilm validate it artificial BM OoCs. Its thickness guarantees intimate contact, key feature mimic blood–brain paracrine signaling types. In summary, hold potential improving accuracy relevance OoC drug discovery tools.

Язык: Английский

Процитировано

4

The Modular µSiM Reconfigured: Integration of Microfluidic Capabilities to Study In Vitro Barrier Tissue Models under Flow DOI
Mehran Mansouri, Adeel Ahmed, S. Danial Ahmad

и другие.

Advanced Healthcare Materials, Год журнала: 2022, Номер 11(21)

Опубликована: Авг. 12, 2022

Microfluidic tissue barrier models have emerged to address the lack of physiological fluid flow in conventional "open-well" Transwell-like devices. However, microfluidic techniques not achieved widespread usage bioscience laboratories because they are fully compatible with traditional experimental protocols. To advance research, there is a need for platform that combines key advantages both open-well and systems. Here, plug-and-play module developed introduce on-demand capabilities an device features nanoporous membrane live-cell imaging capabilities. The magnetic latching assembly this design enables bi-directional reconfiguration allows users conduct experiment format established protocols then add or remove as desired. This work also provides experimentally-validated model select conditions based on needs. As proof-of-concept, flow-induced alignment endothelial cells expression shear-sensitive gene targets demonstrated, different phases neutrophil transmigration across chemically stimulated monolayer under visualized. With these capabilities, it anticipated engineering will adopt reconfigurable due compatibility standard

Язык: Английский

Процитировано

16