Advanced Healthcare Materials,
Год журнала:
2022,
Номер
11(21)
Опубликована: Авг. 12, 2022
Microfluidic
tissue
barrier
models
have
emerged
to
address
the
lack
of
physiological
fluid
flow
in
conventional
"open-well"
Transwell-like
devices.
However,
microfluidic
techniques
not
achieved
widespread
usage
bioscience
laboratories
because
they
are
fully
compatible
with
traditional
experimental
protocols.
To
advance
research,
there
is
a
need
for
platform
that
combines
key
advantages
both
open-well
and
systems.
Here,
plug-and-play
module
developed
introduce
on-demand
capabilities
an
device
features
nanoporous
membrane
live-cell
imaging
capabilities.
The
magnetic
latching
assembly
this
design
enables
bi-directional
reconfiguration
allows
users
conduct
experiment
format
established
protocols
then
add
or
remove
as
desired.
This
work
also
provides
experimentally-validated
model
select
conditions
based
on
needs.
As
proof-of-concept,
flow-induced
alignment
endothelial
cells
expression
shear-sensitive
gene
targets
demonstrated,
different
phases
neutrophil
transmigration
across
chemically
stimulated
monolayer
under
visualized.
With
these
capabilities,
it
anticipated
engineering
will
adopt
reconfigurable
due
compatibility
standard
Islet
transplantation
has
now
become
a
promising
treatment
for
insulin-deficient
diabetes
mellitus.
Compared
to
traditional
treatments,
cell
therapy
can
restore
endogenous
insulin
supplementation,
but
its
large-scale
clinical
application
is
impeded
by
donor
shortages,
immune
rejection,
and
unsuitable
sites.
To
overcome
these
challenges,
an
increasing
number
of
studies
have
attempted
transplant
hydrogel-encapsulated
islet
cells
treat
diabetes.
This
review
mainly
focuses
on
the
strategy
pancreatic
diabetic
therapy,
including
different
sources
encapsulated
in
hydrogels,
encapsulation
methods,
hydrogel
types,
series
accessorial
manners
improve
outcomes.
In
addition,
formation
challenges
as
well
prospects
are
also
presented.
Biomaterials Research,
Год журнала:
2022,
Номер
26(1)
Опубликована: Сен. 30, 2022
The
human
blood-brain
barrier
(BBB)
is
a
unique
multicellular
structure
that
in
critical
demand
for
fundamental
neuroscience
studies
and
therapeutic
evaluation.
Despite
substantial
achievements
creating
vitro
BBB
platforms,
challenges
generating
specifics
of
physiopathological
relevance
are
viewed
as
impediments
to
the
establishment
models.
In
this
review,
we
provide
insight
into
development
deployment
models
allow
investigation
physiology
pathology
neurological
avenues.
First,
highlight
components,
including
cell
sources,
biomaterial
glue
collections,
engineering
techniques
reconstruct
miniaturized
BBB.
Second,
describe
recent
breakthroughs
mini-BBBs
investigating
biological
mechanisms
neurology.
Finally,
discuss
application
medical
approaches.
This
review
provides
strategies
understanding
diseases,
validation
model
drug
discovery,
potential
approach
personalized
medicine.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(10), С. 13006 - 13017
Опубликована: Фев. 28, 2024
Organs-on-chips
(OoCs)
support
an
organotypic
human
cell
culture
in
vitro.
Precise
representation
of
basement
membranes
(BMs)
is
critical
for
mimicking
physiological
functions
tissue
interfaces.
Artificial
polyester
(PES)
and
polycarbonate
(PC)
commonly
used
vitro
models
OoCs
do
not
replicate
the
characteristics
natural
BMs,
such
as
submicrometric
thickness,
selective
permeability,
elasticity.
This
study
introduces
porous
poly(d,l-lactic
acid)
(PDLLA)
nanofilms
replicating
BMs
demonstrates
their
integration
into
microfluidic
chips.
Using
roll-to-roll
gravure
coating
polymer
phase
separation,
we
fabricated
transparent
∼200
nm
thick
PDLLA
films.
These
are
60
times
thinner
27
more
elastic
than
PES
show
uniformly
distributed
pores
controlled
diameter
(0.4
to
1.6
μm),
which
favor
compartmentalization
exchange
large
water-soluble
molecules.
Human
umbilical
vein
endothelial
cells
(HUVECs)
on
stretched
across
microchannels
exhibited
97%
viability,
enhanced
adhesion,
a
higher
proliferation
rate
compared
performance
glass
substrates.
After
5
days
culture,
HUVECs
formed
functional
barrier
suspended
nanofilms,
confirmed
by
10-fold
increase
transendothelial
electrical
resistance
blocked
150
kDa
dextran
diffusion.
When
integrated
between
two
channels
exposed
shear
stress,
despite
ultrathin
upheld
integrity
efficiently
maintained
separation
channels.
The
successful
formation
adherent
endothelium
coculture
astrocytes
either
side
nanofilm
validate
it
artificial
BM
OoCs.
Its
thickness
guarantees
intimate
contact,
key
feature
mimic
blood–brain
paracrine
signaling
types.
In
summary,
hold
potential
improving
accuracy
relevance
OoC
drug
discovery
tools.
Advanced Healthcare Materials,
Год журнала:
2022,
Номер
11(21)
Опубликована: Авг. 12, 2022
Microfluidic
tissue
barrier
models
have
emerged
to
address
the
lack
of
physiological
fluid
flow
in
conventional
"open-well"
Transwell-like
devices.
However,
microfluidic
techniques
not
achieved
widespread
usage
bioscience
laboratories
because
they
are
fully
compatible
with
traditional
experimental
protocols.
To
advance
research,
there
is
a
need
for
platform
that
combines
key
advantages
both
open-well
and
systems.
Here,
plug-and-play
module
developed
introduce
on-demand
capabilities
an
device
features
nanoporous
membrane
live-cell
imaging
capabilities.
The
magnetic
latching
assembly
this
design
enables
bi-directional
reconfiguration
allows
users
conduct
experiment
format
established
protocols
then
add
or
remove
as
desired.
This
work
also
provides
experimentally-validated
model
select
conditions
based
on
needs.
As
proof-of-concept,
flow-induced
alignment
endothelial
cells
expression
shear-sensitive
gene
targets
demonstrated,
different
phases
neutrophil
transmigration
across
chemically
stimulated
monolayer
under
visualized.
With
these
capabilities,
it
anticipated
engineering
will
adopt
reconfigurable
due
compatibility
standard
