Macrophage based drug delivery: Key challenges and strategies

Bioactive Materials, Год журнала: 2024, Номер 38, С. 55 - 72

Опубликована: Апрель 23, 2024

As a natural immune cell and antigen presenting cell, macrophages have been studied engineered to treat human diseases. Macrophages are well-suited for use as drug carriers because of their biological characteristics, such excellent biocompatibility, long circulation, intrinsic inflammatory homing phagocytosis. Meanwhile, macrophages' uniquely high plasticity easy re-education polarization facilitates part efficacious therapeutics the treatment diseases or tumors. Although recent studies demonstrated promising advances in macrophage-based delivery, several challenges currently hinder further improvement therapeutic effect clinical application. This article focuses on main utilizing from selection macrophage sources, loading, maintenance phenotypes, migration release at target sites. In addition, corresponding strategies insights related these described. Finally, we also provide perspective shortcomings road translation production.

Язык: Английский

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The pursuit of linear dosage in pharmacy: reservoir-based drug delivery systems from macro to micro scale

Expert Opinion on Drug Delivery, Год журнала: 2025, Номер unknown, С. 1 - 20

Опубликована: Янв. 7, 2025

Introduction The pursuit of linear dosage in pharmacy is essential for achieving consistent therapeutic release and enhancing patient compliance. This review provides a comprehensive summary zero-order drug delivery systems, with particular focus on reservoir-based systems emanated from different microfabrication technologies.

Язык: Английский

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Tumor-associated macrophages in nanomaterial-based anti-tumor therapy: as target spots or delivery platforms

Frontiers in Bioengineering and Biotechnology, Год журнала: 2023, Номер 11

Опубликована: Авг. 16, 2023

Targeting tumor-associated macrophages (TAMs) has emerged as a promising approach in cancer therapy. This article provides comprehensive review of recent advancements the field nanomedicines targeting TAMs. According to crucial role TAMs tumor progression, strategies inhibit macrophage recruitment, suppress TAM survival, and transform phenotypes are discussed potential therapeutic avenues. To enhance capacity nanomedicines, various approaches such use ligands, immunoglobulins, short peptides explored. The utilization live programmed macrophages, cell membrane-coated nanoparticles macrophage-derived extracellular vesicles drug delivery platforms is also highlighted, offering improved biocompatibility prolonged circulation time. However, challenges remain achieving precise controlled release. heterogeneity variability surface markers pose hurdles specific recognition. Furthermore, safety clinical applicability these requires further investigation. In conclusion, hold great promise therapy, enhanced specificity reduced side effects. Addressing existing limitations expanding our understanding biology will pave way for successful translation nano-therapies into practice.

Язык: Английский

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Multiple dyes applications for fluorescent convertible polymer capsules as macrophages tracking labels

Heliyon, Год журнала: 2024, Номер 10(10), С. e30680 - e30680

Опубликована: Май 1, 2024

Tracing individual cell pathways among the whole population is crucial for understanding their behavior, communication, migration dynamics, and fate. Optical labeling one approach tracing cells, but it typically requires genetic modification to induce generation of photoconvertible proteins. Nevertheless, this has limitations not applicable certain types. For instance, often leads death macrophages. This study aims develop an alternative method macrophages by utilizing micron-sized capsules capable easy internalization prolonged retention within cells. Thermal treatment in a polyvinyl alcohol gel medium employed scalable synthesis with wide range fluorescent dyes, including rhodamine 6G, pyronin B, fluorescein, acridine yellow, orange, thiazine red, previously reported B. The fluorescence brightness, photostability, photoconversion ability are evaluated using confocal laser scanning microscopy. Viability, uptake, mobility, studies conducted on RAW 264.7 bone marrow-derived macrophages, serving as model lines. production yield increased due use gel, eliminating need conventional filtration steps. Capsules entrapping B 6G meet all requirements intracellular tracking. Mass spectrometry analysis reveals sequence deethylation steps that result blue shifts dye spectra upon irradiation. Cellular demonstrate robust uptake capsules. exhibit minimal cytotoxicity have negligible impact motility. successful RhB-containing cells highlights potential alternatives proteins labeling, promising applications personalized medicine.

Язык: Английский

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The Evolution of Microfluidic‐Based Drug‐Loading Techniques for Cells and Their Derivatives

Small, Год журнала: 2024, Номер 20(47)

Опубликована: Авг. 17, 2024

Conventional drug delivery techniques face challenges related to targeting and adverse reactions. Recent years have witnessed significant advancements in nanoparticle-based carriers. Nevertheless, concerns persist regarding their safety insufficient metabolism. Employing cells derivatives, such as cell membranes extracellular vesicles (EVs), carriers effectively addresses the associated with nanoparticle However, an essential hurdle remains efficiently loading drugs into these With advancement of microfluidic technology its advantages precise manipulation at micro- nanoscales, well minimal sample loss, it has found extensive application using thereby fostering development drug-loading techniques. This paper outlines characteristics benefits utilizing derivatives provides overview current techniques, particularly those rooted technology. The potential for targeted disease therapy through systems employing is foreseen.

Язык: Английский

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Brain neurons internalise polymeric micron-sized capsules: insights from in vitro and in vivo studies

Materials Today Bio, Год журнала: 2025, Номер 31, С. 101493 - 101493

Опубликована: Янв. 22, 2025

Nanoengineered encapsulation presents a promising strategy for targeted drug delivery to specific regions in the body. While polyelectrolyte-based biodegradable microcapsules can achieve highly localised release tissues and cell cultures, delivering drugs intracellular sites brain remains significant challenge. In this study, we utilized advanced imaging techniques, both vitro vivo, investigate whether neurons internalise designed delivery. High-resolution live-cell revealed that differentiating N2A cells actively microcapsules, often incorporating multiple capsules per cell. Likewise, primary hippocampal cortical were observed effectively polymeric microcapsules. intact brain, multiplexed two-photon excitation vivo confirmed internalisation of by following somatosensory region. This was time-dependent, correlated with particle size mediated macropinocytosis mechanism appears bypass lysosomal formation. Importantly, presence internalised did not impair neuronal function, as maintained normal firing activity action potential characteristics. Furthermore, no adverse effects after week microcapsule mouse brain. Our findings indicate are effective safe carriers neurons, providing approach therapeutic applications.

Язык: Английский

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Engineering colloidal systems for cell manipulation, delivery, and tracking

Advances in Colloid and Interface Science, Год журнала: 2025, Номер 340, С. 103462 - 103462

Опубликована: Фев. 27, 2025

Язык: Английский

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Mannose‐Glycated Metal‐Phenolic Microcapsules Orchestrate Phenotype Switch of Macrophages for Boosting Tumor Immunotherapy

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Фев. 27, 2025

Abstract Microcapsules are advancing in immunotherapy, with both their core and shell being capable of loading immunoregulatory substances. Notably, microcapsules intrinsic bioactivities can more directly modulate the immune microenvironment, while current research this area remains scarce. Herein, immunomodulatory metal‐phenolic (mMPMs) is developed through one‐step assembly dopamine‐modified hyaluronic acid (HADA) Fe III onto mannose‐glycated bovine serum albumin microbubbles (Man‐BSA MBs). Specifically, Man‐BSA formed during early stages Maillard reaction sonicated to produce as templates for capsule preparation. Subsequently, HADA rapidly coated on coordinates form after air escapes from MBs. Mass spectrometry analysis identifies abundant lysine glycation sites Man‐BSA, highest site percentage reaching 94.88%. within mMPMs effectively promotes macrophage internalization, induces accumulation pro‐inflammatory mediators, thereby results M1 polarization macrophages, further corroborated by proteomic analysis. Consequently, compelling anti‐tumor effects demonstrated vitro vivo. Overall, work presents an microcapsule that activates phenotype which a promising microcarrier improve immunotherapeutic effects.

Язык: Английский

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Fluorescent polymer markers photoconvertible with a 532 nm laser for individual cell labeling

Journal of Biophotonics, Год журнала: 2023, Номер 16(6)

Опубликована: Фев. 2, 2023

Abstract Fluorescent photoconvertible materials and molecules have been successfully exploited as bioimaging markers cell trackers. Recently, the novel fluorescent polymer developed that allow long‐term tracking of individual labeled cells. However, it is still necessary to study functionality this type labels for various operating conditions, in particular commonly used discrete wavelength lasers. In article, photoconversion with both pulsed continuous‐wave lasers 532 nm‐irradiation wavelength, under different laser power densities were studied. The process was described its possible mechanism proposed. peculiarities capsules performance an aqueous suspension a single capsule described. We performed successful nondestructivity marker inside RAW 264.7 monocyte/macrophage cells showing prospects these live labeling.

Язык: Английский

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Magnetic Platelets as a Platform for Drug Delivery and Cell Trapping

Pharmaceutics, Год журнала: 2023, Номер 15(1), С. 214 - 214

Опубликована: Янв. 7, 2023

The possibility of using magnetically labeled blood cells as carriers is a novel approach in targeted drug-delivery systems, potentially allowing for improved bloodstream delivery strategies. Blood already meet the requirements biocompatibility, safety from clotting and blockage small vessels. It would solve important problem patient's immune response to embedded foreign carriers. high efficiency platelet loading makes them promising research objects development personalized systems. We are developing new use platelets decorated with magnetic nanoparticles system, focus on delivery. Platelets non-nuclear great importance pathogenesis blood-clotting disorders. In addition, able attach circulating tumor cells. this article, we studied effect BSA-modified healthy cancer This opens up broad prospects future based specific active substances by method.

Язык: Английский

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