Materials Today Bio,
Год журнала:
2025,
Номер
32, С. 101687 - 101687
Опубликована: Март 20, 2025
Repairing
bone
defects
in
inflammatory
conditions
remains
a
significant
clinical
challenge.
An
ideal
scaffold
material
for
such
situations
should
enable
minimally
invasive
implantation
and
integrate
capabilities
immunomodulation,
anti-infection
therapy,
enhanced
regeneration.
In
this
study,
we
developed
injectable
calcitriol@polydopamine@gelatin
methacryloyl
hydrogel
microspheres
(CAL@PDA@GMs)
using
microfluidic
technology.
This
system
facilitates
the
sustained
release
of
calcitriol,
which
features
excellent
biocompatibility
biodegradability,
promotes
osteogenesis,
scavenges
excessive
reactive
oxygen
species
(ROS),
induces
polarization
macrophages
from
M1
to
M2
phenotype,
thereby
mitigating
lipopolysaccharide
(LPS)-induced
inflammation.
These
mechanisms
work
synergistically
create
an
optimal
immune
microenvironment
regeneration
conditions.
RNA
sequencing
(RNA-Seq)
analyses
revealed
that
immunomodulation
is
achieved
by
regulating
macrophage
phenotypes,
inhibiting
nuclear
transcription
factor-kappa
B
(NF-κB)
ROS
signaling
pathways,
reducing
secretion
pro-inflammatory
cytokines.
study
proposes
novel
method
enhance
tissue
remediating
damaged
presents
potential
therapeutic
strategy
large-scale
injuries.
Low
back
pain
(LBP)
resulting
from
sciatic
nerve
compression
presents
major
challenges
in
management,
as
traditional
therapies
provide
only
short-term
relief
and
pose
risks
of
systemic
toxicity.
In
this
study,
an
innovative
Fe3O4@ZIF-8-RVC
(FZR)
dual-responsive
nanoplatform
is
introduced
that
integrates
magnetic
targeting
with
pH-sensitive,
sustained
drug
release
to
overcome
these
limitations.
The
FZR
encapsulates
ropivacaine
(RVC)
within
the
ZIF-8-coated
Fe3O4
core,
enabling
precise
prolonged
analgesia
at
injury
site
through
guidance
acid-triggered
release.
vitro
vivo
assessments
indicate
achieves
high
loading,
acidic
environments,
excellent
biocompatibility,
significantly
extending
analgesic
effects
chronic
models
while
minimizing
exposure.
Behavioral
tests
molecular
analyses
LBP
rat
confirm
effectively
suppresses
pain-related
neuronal
activity
central
sensitization
markers.
This
offers
a
safe,
long-lasting,
targeted
therapeutic
approach,
holding
strong
potential
for
advancing
related
neuropathic
conditions.
Materials Today Bio,
Год журнала:
2025,
Номер
32, С. 101687 - 101687
Опубликована: Март 20, 2025
Repairing
bone
defects
in
inflammatory
conditions
remains
a
significant
clinical
challenge.
An
ideal
scaffold
material
for
such
situations
should
enable
minimally
invasive
implantation
and
integrate
capabilities
immunomodulation,
anti-infection
therapy,
enhanced
regeneration.
In
this
study,
we
developed
injectable
calcitriol@polydopamine@gelatin
methacryloyl
hydrogel
microspheres
(CAL@PDA@GMs)
using
microfluidic
technology.
This
system
facilitates
the
sustained
release
of
calcitriol,
which
features
excellent
biocompatibility
biodegradability,
promotes
osteogenesis,
scavenges
excessive
reactive
oxygen
species
(ROS),
induces
polarization
macrophages
from
M1
to
M2
phenotype,
thereby
mitigating
lipopolysaccharide
(LPS)-induced
inflammation.
These
mechanisms
work
synergistically
create
an
optimal
immune
microenvironment
regeneration
conditions.
RNA
sequencing
(RNA-Seq)
analyses
revealed
that
immunomodulation
is
achieved
by
regulating
macrophage
phenotypes,
inhibiting
nuclear
transcription
factor-kappa
B
(NF-κB)
ROS
signaling
pathways,
reducing
secretion
pro-inflammatory
cytokines.
study
proposes
novel
method
enhance
tissue
remediating
damaged
presents
potential
therapeutic
strategy
large-scale
injuries.
