Analytica Chimica Acta, Год журнала: 2024, Номер 1303, С. 342530 - 342530
Опубликована: Март 25, 2024
Язык: Английский
Talanta, Год журнала: 2023, Номер 269, С. 125414 - 125414
Опубликована: Ноя. 17, 2023
Язык: Английский
Процитировано
13
Talanta, Год журнала: 2024, Номер 273, С. 125938 - 125938
Опубликована: Март 16, 2024
Язык: Английский
Процитировано
5
Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Год журнала: 2024, Номер 16(3)
Опубликована: Май 1, 2024
Abstract MicroRNA (miRNA), crucial non‐coding RNAs, have emerged as key biomarkers in molecular diagnostics, prognosis, and personalized medicine due to their significant role gene expression regulation. Salivary miRNA, particular, stands out for its non‐invasive collection method ease of accessibility, offering promising avenues the development point‐of‐care diagnostics a spectrum diseases, including cancer, neurodegenerative disorders, infectious diseases. Such promises rapid precise diagnosis, enabling timely treatment. Despite advancements salivary miRNA‐based testing, challenges persist quantification, multiplexing, sensitivity, specificity, particularly miRNA at low concentrations complex biological mixtures. This work delves into these challenges, focusing on application tests use. We explore biogenesis analyze quantitative disease relevance infection, disorders. also examined recent progress extraction, amplification, multiplexed detection methods. study offers comprehensive view testing (POCT). Its successful advancement could revolutionize early detection, monitoring, management various conditions, enhancing healthcare outcomes. article is categorized under: Diagnostic Tools > Biosensing Nanodevices
Язык: Английский
Процитировано
5
Sensors and Actuators B Chemical, Год журнала: 2024, Номер unknown, С. 136607 - 136607
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
4
Biosensors and Bioelectronics, Год журнала: 2024, Номер 267, С. 116803 - 116803
Опубликована: Сен. 19, 2024
Язык: Английский
Процитировано
3
Chemical Engineering Journal, Год журнала: 2025, Номер 506, С. 159756 - 159756
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
ACS Sensors, Год журнала: 2025, Номер unknown
Опубликована: Фев. 14, 2025
Strand displacement amplification (SDA) is an isothermal DNA technique. Herein, we developed a novel SDA system, designated All-U-Want (AUW-SDA), which was used as signal strategy for the construction of nucleic acid detection biosensors. AUW-SDA capable target turnover and can be utilized sequences without available 3′-ends. Of particular significance ability to generate substantial number programmable in accordance with specifications sensor output methods, irrespective sequence acid. We N gene SARS-CoV-2 model develop sensing platform dual outputs. The colorimetric signals were generated by G-quadruplex/hemin DNAzyme, G-rich produced C-rich primer. On other hand, altering within replaceable region primer, activator obtained from AUW-SDA, could trigger activity CRISPR/Cas12a, cleaving probes modified fluorophore quencher at each end subsequently yielding fluorescent signals. After reaction conditions optimized, limit (LOD) values assays estimated 0.672 fM 13.3 fM, respectively. biosensors biological sample detection. reliability proposed method validated against RT-qPCR results. In addition, portable scanner-assisted high-throughput RGB analysis (PSHRA) developed. This applied our biosensor multilocus SARS-CoV-2. results satisfactory, indicating potential this approach field testing or point-of-care (POC) diagnostics.
Язык: Английский
Процитировано
0
Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113055 - 113055
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0
Biosensors, Год журнала: 2025, Номер 15(3), С. 155 - 155
Опубликована: Март 2, 2025
High-sensitivity and high-specificity biodetection is critical for advancing applications in life sciences, biosafety, food safety, environmental monitoring. CRISPR/Cas systems have emerged as transformative tools biosensing due to their unparalleled specificity, programmability, unique enzymatic activities. They exhibit two key cleavage behaviors: precise ON-target guided by specific protospacers, which ensures accurate target recognition, bystander activity triggered upon binding, enables robust signal amplification. These properties make highly versatile designing biosensors ultra-sensitive detection. This review comprehensively explores recent advancements system-based biosensors, highlighting impact on improving performance. We discuss the integration of with diverse readout mechanisms, including electrochemical, fluorescent, colorimetric, surface-enhanced Raman scattering (SERS), so on. Additionally, we examine development integrated systems, such microfluidic devices portable leverage technology point-of-care testing (POCT) high-throughput analysis. Furthermore, identify unresolved challenges, aiming inspire innovative solutions accelerate translation these technologies into practical diagnostics, food, environment safety.
Язык: Английский
Процитировано
0
Science Advances, Год журнала: 2025, Номер 11(18)
Опубликована: Май 2, 2025
Affordable, sensitive, and simplified DNA/RNA detection is important for disease diagnosis enables timely medical intervention measures. Usually, high sensitivity depends on expensive instruments sophisticated procedures, making contradict affordability simplicity. Here, we proposed an ultra-sensitive single-tube biosensor (USTB) where users can visually detect targets by observing the liquid motion state in a glass tube. The developed instrument-free USTB performed low-cost ($0.1), fast (1 min), both fragments (≤1 aM) clinical positive samples, which commercial reverse transcription polymerase chain reaction (RT-PCR) PCR kits could not effectively recognize. Furthermore, promising to be easily applied other-type biomarkers designed smart sensing unit.
Язык: Английский
Процитировано
0