Self-Assembly of Multimolecular Components for Engineering Enzyme-Mimetic Materials

Accounts of Materials Research, Год журнала: 2024, Номер 5(9), С. 1072 - 1086

Опубликована: Июль 4, 2024

ConspectusNatural enzymes, with their intricate three-dimensional structures, facilitate a wide array of biochemical reactions exceptional precision and speed. The catalytic capabilities enzymes arise from the distinctive structures active sites, where functional groups collaborate or aid cofactors (organic ionic) in binding substrates specificity catalyzing transformations. Inspired by structure–function relationship supramolecular self-assembly, bottom-up approach nanofabrication, has been employed to create enzyme-mimetic catalysts. However, accurately replicating enzymatic sites poses formidable challenge, primarily because intricacies mimicking complexity natural protein folding.Many biological systems, such as tryptophan synthase ribosomes, rely on association multiple component subunits, each maintaining its structural integrity, enable efficient versatile functionalities. hierarchical self-assembly principles observed these systems have inspired us design self-assemble complementary molecular building blocks that form individual folding aggregating allowing for precise control over distribution reactive enzyme-like sites. customization either without disrupting enables flexible engineering properties. This Account will focus employing components, drawing research progress our lab, construct catalysts built-in metal-dependent metal-free be highlighted.To fabricate heme pockets copper within synthetic materials, we scaffold stabilizing hemin forming cluster, followed introduction second enhance substrate adsorption metal reactivity. resulting enzyme mimics exhibit remarkable synergistic activities possess great stability against harsh conditions, high temperatures, ionic strength, cyclic acidification/neutralization treatment. They can engineered tailorable selectivity toward specific chirality sizes externally stimulated switch between ON/OFF states. These shown performances sensing biomolecules interest, biomass degradation, aiding understanding mechanism native enzymes. To achieve catalysis, introduce "driving" guide formation assemblies activity hydrolases, photodecarboxylase, photo-oxidase, applications peptide modifications antibacterial therapy. Moreover, organized components like histidine catalyze achieved heme-dependent providing insights into novel biocatalytic mechanisms. Additionally, discuss DNAzyme units DNA nanostructured templates, which provide suitable microenvironments fabrication polymer nanopattern well-defined shapes.In end, key challenges related modeling, enhancing performance, increasing We also propose future perspectives achieving high-value practical applications. Our collective efforts outline strategies developing robust catalysts, general methods may extend other aiming mimic catalysis.

Язык: Английский

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A novel fluorescence and colorimetric dual sensing system for rapid and sensitive detection of histidine based on TSPP-CA

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Год журнала: 2025, Номер unknown, С. 126145 - 126145

Опубликована: Март 1, 2025

Язык: Английский

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Advanced Colorimetric Sensors: Cutting-Edge Materials and Smart Approaches

IntechOpen eBooks, Год журнала: 2024, Номер unknown

Опубликована: Дек. 4, 2024

Color is a captivating aspect of our perception, profoundly influencing how we interact with and interpret the world. As fundamental element visual experience, it shapes emotions, preferences, even physiological responses. Beyond its esthetic emotional impact, color serves practical purposes across various fields, including medicine for diagnostics therapeutic applications in industrial sectors quality control product identification. Colorimetric sensors that are user-friendly, portable, instrument-free, low cost, yet provide sensitive selective detection analytes, essential point-of-need point-of-care applications. This book chapter will discuss smart materials approaches used colorimetric measurement-based biosensor methods biomedical, diagnostic, food safety, environmental pollution

Язык: Английский

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Dual-Enzyme-Mimic Activity of FeCo-MOF for Colorimetric Detection of Cysteine and Cu2+

Colloids and Surfaces A Physicochemical and Engineering Aspects, Год журнала: 2024, Номер unknown, С. 136036 - 136036

Опубликована: Дек. 1, 2024

Язык: Английский

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