Immobilized Protein Strategies Based on Cell Membrane Chromatography and its Application in Discovering Active and Toxic Substances in Traditional Chinese Medicine DOI Creative Commons

Yi Shan,

Jiayu Lu, Qian Hua

и другие.

Pharmacological Research, Год журнала: 2024, Номер 210, С. 107492 - 107492

Опубликована: Ноя. 2, 2024

Traditional Chinese medicine (TCM) contributes significantly to human health. Owing the complexity of ingredients in TCM, it is necessary conduct basic research on effective substances and identify toxic control safety medication. Cell membrane chromatography (CMC) an important method for identifying target components complex systems. The cell stationary phase (CMSP) core component key factor determining effectiveness CMC. This review summarizes development CMSP with different protein immobilization strategies application CMC discovery active aim providing means quality TCM.

Язык: Английский

Cell Membrane-Coated Nanoparticles for Precision Medicine: A Comprehensive Review of Coating Techniques for Tissue-Specific Therapeutics DOI Open Access
Andrés Fernández-Borbolla, Lorena Garcı́a-Hevia, Mónica L. Fanárraga

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(4), С. 2071 - 2071

Опубликована: Фев. 8, 2024

Nanoencapsulation has become a recent advancement in drug delivery, enhancing stability, bioavailability, and enabling controlled, targeted substance delivery to specific cells or tissues. However, traditional nanoparticle faces challenges such as short circulation time immune recognition. To tackle these issues, cell membrane-coated nanoparticles have been suggested practical alternative. The production process involves three main stages: lysis membrane fragmentation, isolation, coating. Cell membranes are typically fragmented using hypotonic with homogenization sonication. Subsequent fragments isolated through multiple centrifugation steps. Coating can be achieved extrusion, sonication, combination of both methods. Notably, this analysis reveals the absence universally applicable method for coating, stages differ significantly their procedures. This review explores current developments approaches nanoparticles, highlighting potential an effective alternative various therapeutic applications.

Язык: Английский

Процитировано

17

Engineered Cell Membrane-Camouflaged Nanomaterials for Biomedical Applications DOI Creative Commons
Xiaoxuan Guan, Simin Xing, Yang Liu

и другие.

Nanomaterials, Год журнала: 2024, Номер 14(5), С. 413 - 413

Опубликована: Фев. 23, 2024

Recent strides in nanomaterials science have paved the way for creation of reliable, effective, highly accurate, and user-friendly biomedical systems. Pioneering integration natural cell membranes into sophisticated nanocarrier architectures, membrane camouflage has emerged as a transformative approach regulated drug delivery, offering benefits minimal immunogenicity coupled with active targeting capabilities. Nevertheless, utility such is curtailed by challenges like suboptimal precision lackluster therapeutic efficacy. Tailored engineering stands at forefront biomedicine, equipping nanoplatforms capacity to conduct more complex operations. This review commences an examination prevailing methodologies engineering, spotlighting strategies direct chemical modification, lipid insertion, hybridization, metabolic glycan labeling, genetic engineering. Following this, evaluation unique attributes various presented, delivering in-depth scrutiny substantial advancements applications driven cutting-edge engineered camouflage. The discourse culminates recapitulating salient influence within nanomaterial prognosticates its seminal role healthcare technologies. It envisaged that insights offered herein will catalyze novel avenues innovation refinement camouflaged nanotechnologies.

Язык: Английский

Процитировано

10

Nanomaterials in gastric cancer: pioneering precision medicine for diagnosis, therapy, and prevention DOI

Tiantian Liu,

Ying-Qiu Gu, Zaili Yang

и другие.

Medical Oncology, Год журнала: 2025, Номер 42(4)

Опубликована: Март 6, 2025

Язык: Английский

Процитировано

1

Development of target-based cell membrane affinity ultrafiltration technology for a simplified approach to discovering potential bioactive compounds in natural products DOI
Xuan Chen, Jian‐Liang Zhou,

Jinhao Yu

и другие.

Analytical and Bioanalytical Chemistry, Год журнала: 2024, Номер 416(7), С. 1647 - 1655

Опубликована: Фев. 2, 2024

Язык: Английский

Процитировано

2

Construction of biomimetic camouflaged neutrophil membrane nanoparticles for precise delivery and augmented glioma cancer treatment DOI

Yongyan Bi,

Peiyu Qian,

Zuopeng Su

и другие.

Process Biochemistry, Год журнала: 2024, Номер 145, С. 229 - 242

Опубликована: Июнь 18, 2024

Язык: Английский

Процитировано

2

Microfluidics‐Driven Manufacturing and Multiscale Analytical Characterization of Nanoparticle‐Vesicle Hybrids DOI Creative Commons
Jacopo Cardellini, Karl Normak, Michael Gerlt

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер 14(4)

Опубликована: Дек. 25, 2024

Coating synthetic nanoparticles (NPs) with lipid membranes is a promising approach to enhance the performance of nanomaterials in various biological applications, including therapeutic delivery target organs. Current methods for achieving this coating often rely on bulk approaches which can result low efficiency and poor reproducibility. Continuous processes coupled quality control represent an attractive strategy manufacture products consistent attributes high yields. Here, concept implemented by developing acoustic microfluidic device together analytical platform prepare nanoparticle-vesicle hybrids quantitatively characterize nanoparticle coverage using fluorescence-based techniques at different levels resolution. With polymethyl methacrylate (PMMA) are successfully coated liposomes extracellular vesicles (EVs), encapsulation 70%. Moreover, enables identification design rules tuning operational parameters material properties, buffer composition, nanoparticle/vesicle ratio, vesicle rigidity.

Язык: Английский

Процитировано

2

Chemical conjugation innovations for protein nanoparticles DOI
Mark Legendre,

Virginia H. Pistilli,

Gözde S. Demirer

и другие.

Trends in Chemistry, Год журнала: 2024, Номер 6(8), С. 470 - 486

Опубликована: Июль 4, 2024

Язык: Английский

Процитировано

0

Research trends in functionalized Fe3O4 composites based on affinity recognition systems for targeted extraction of natural products DOI
Haiwei Wang, Jiang Xu, Yi Qin

и другие.

Journal of Chromatography A, Год журнала: 2024, Номер 1730, С. 465145 - 465145

Опубликована: Июль 8, 2024

Язык: Английский

Процитировано

0

Bioorthogonal Synthesis of Biomimetic Nanoparticles for Screening Chemical Hazards in Food Samples DOI
Qianyu Li, Jianghua Liu,

Zihe Qi

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 97(1), С. 247 - 254

Опубликована: Дек. 30, 2024

The ability to identify unknown risks is the key improving level of food safety. However, conventional nontargeted screening methods for new contaminant identification and risk assessment remain difficult work. Herein, a toxic-oriented platform based on high-expression epidermal growth factor receptor HEK293 cell membrane-coated magnetic nanoparticles (EGFR/MNPs) was first used discovery contaminants from samples. EGFR served as bait bind compounds, considering that abnormal activation related incidence numerous chronic inflammatory diseases. membranes were specifically covalently immobilized carriers through metabolic glycoengineering strain-promoted alkyne–azide cycloaddition (SPAAC) ensure stability bioactivity EGFR/MNPs. synthesized EGFR/MNPs possessed an excellent loading amount screen potential substances thermally processed food. Finally, two harman norharman, rapidly identified. toxicological experiments confirmed screened compounds could promote phosphorylation further activate downstream signaling pathways, thereby inducing cellular oxidative damage. proposed method provided identifying

Язык: Английский

Процитировано

0

Immobilized Protein Strategies Based on Cell Membrane Chromatography and its Application in Discovering Active and Toxic Substances in Traditional Chinese Medicine DOI Creative Commons

Yi Shan,

Jiayu Lu, Qian Hua

и другие.

Pharmacological Research, Год журнала: 2024, Номер 210, С. 107492 - 107492

Опубликована: Ноя. 2, 2024

Traditional Chinese medicine (TCM) contributes significantly to human health. Owing the complexity of ingredients in TCM, it is necessary conduct basic research on effective substances and identify toxic control safety medication. Cell membrane chromatography (CMC) an important method for identifying target components complex systems. The cell stationary phase (CMSP) core component key factor determining effectiveness CMC. This review summarizes development CMSP with different protein immobilization strategies application CMC discovery active aim providing means quality TCM.

Язык: Английский

Процитировано

0