Noninvasive in vivo imaging of macrophages: understanding tumor microenvironments and delivery of therapeutics
Biomarker Research,
Год журнала:
2025,
Номер
13(1)
Опубликована: Янв. 26, 2025
Abstract
Macrophages
are
pivotal
in
the
body’s
defense
and
response
to
inflammation.
They
present
significant
numbers
widely
implicated
various
diseases,
including
cancer.
While
molecular
histological
techniques
have
advanced
our
understanding
of
macrophage
biology,
their
precise
function
within
cancerous
microenvironments
remains
underexplored.
Enhancing
knowledge
macrophages
dynamics
extracellular
vesicles
(EVs)
cancer
development
can
potentially
improve
therapeutic
management.
Notably,
also
been
harnessed
deliver
drugs.
Noninvasive
vivo
imaging
is
crucial
for
investigating
intricate
cellular
processes,
comprehending
underlying
mechanisms
tracking
cells
EVs’
migration,
devising
macrophage-dependent
drug-delivery
systems
living
organisms.
Thus,
has
become
an
indispensable
tool
biomedical
research.
The
integration
multimodal
approaches
continued
novel
contrast
agents
hold
promise
overcoming
current
limitations
expanding
applications
imaging.
This
study
comprehensively
reviews
several
methods
labeling
modalities,
assessing
merits
drawbacks
each
approach.
review
concludes
by
offering
insights
into
applicability
real
time
monitoring
preclinical
clinical
scenarios.
Язык: Английский
Exosomes in Regulating miRNAs for Biomarkers of Neurodegenerative Disorders
Molecular Neurobiology,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 7, 2025
Язык: Английский
Overview of the characterization of extracellular vesicles
Elsevier eBooks,
Год журнала:
2025,
Номер
unknown, С. 25 - 56
Опубликована: Янв. 1, 2025
Язык: Английский
Electrochemical Biosensors for the Detection of Exosomal microRNA Biomarkers for Early Diagnosis of Neurodegenerative Diseases
Jiacheng Yu,
Runzhi Zhou,
Shan Liu
и другие.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 9, 2025
Early
and
precise
diagnosis
of
neurodegenerative
disorders
like
Alzheimer's
(AD)
Parkinson's
(PD)
is
crucial
for
slowing
their
progression
enhancing
patient
outcomes.
Exosomal
microRNAs
(miRNAs)
are
emerging
as
promising
biomarkers
due
to
ability
reflect
the
diseases'
pathology,
yet
low
abundance
poses
significant
detection
hurdles.
This
review
article
delves
into
burgeoning
field
electrochemical
biosensors,
designed
exosomal
miRNA
biomarkers.
Electrochemical
biosensors
offer
a
compelling
solution,
combining
sensitivity
required
detect
low-abundance
with
specificity
needed
discern
profiles
distinctive
neural
pathological
states.
We
explore
operational
principles
these
including
transduction
mechanisms
that
facilitate
detection.
The
also
summarizes
advancements
in
nanotechnology,
signal
enhancement,
bioreceptor
anchoring,
microfluidic
integration
improve
sensor
accuracy.
evidence
use
disease
analyzed,
focusing
on
clinical
impact,
diagnostic
precision,
obstacles
faced
practical
applications.
Their
potential
point-of-care
testing
regulatory
considerations
market
entry
discussed.
Looking
toward
future,
highlights
forthcoming
innovations
might
revolutionize
early
processes.
impressive
sensitivity,
specificity,
compatibility,
track
become
instrumental
diseases,
possibly
transforming
care
prognosis.
Язык: Английский
Alzheimer’s Disease Pathology and Assistive Nanotheranostic Approaches for Its Therapeutic Interventions
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(17), С. 9690 - 9690
Опубликована: Сен. 7, 2024
Alzheimer’s
disease
(AD)
still
prevails
and
continues
to
increase
indiscriminately
throughout
the
21st
century,
is
thus
responsible
for
depreciating
quality
of
health
associated
sectors.
AD
a
progressive
neurodegenerative
disorder
marked
by
significant
amassment
beta-amyloid
plaques
neurofibrillary
tangles
near
hippocampus,
leading
consequent
loss
cognitive
abilities.
Conventionally,
amyloid
tau
hypotheses
have
been
established
as
most
prominent
in
providing
detailed
insight
into
pathogenesis
revealing
associative
biomarkers
intricately
involved
progression.
Nanotheranostic
deliberates
rational
thought
toward
designing
efficacious
nanosystems
strategic
endeavors
diagnosis
therapeutic
implications.
The
exceeding
advancements
this
field
enable
scientific
community
envisage
conceptualize
pharmacokinetic
monitoring
drug,
sustained
targeted
drug
delivery
responses,
fabrication
anti-amyloid
therapeutics,
enhanced
accumulation
across
blood–brain
barrier
(BBB),
giving
an
optimistic
approach
towards
personalized
precision
medicine.
Current
methods
idealized
on
design
bioengineering
array
nanoparticulate
systems
offer
higher
affinity
neurocapillary
endothelial
cells
BBB.
They
recently
attracted
intriguing
attention
early
diagnostic
measures
taken
manage
progression
disease.
In
article,
we
tend
furnish
comprehensive
outlook,
mechanism
conventional
pathogenesis,
new
findings.
We
also
summarize
shortcomings
diagnostic,
prognostic,
approaches
undertaken
alleviate
AD,
unique
window
nanotheranostic
without
disregarding
potential
drawbacks,
side
effects,
safety
concerns.
Язык: Английский
Engineering Magnetic Extracellular Vesicles Mimetics for Enhanced Targeting Chemodynamic Therapy to Overcome Ovary Cancer
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(30), С. 39021 - 39034
Опубликована: Июль 21, 2024
Chemodynamic
therapy
(CDT),
employing
metal
ions
to
transform
endogenous
H2O2
into
lethal
hydroxyl
radicals
(•OH),
has
emerged
as
an
effective
approach
for
tumor
treatment.
Yet,
its
efficacy
is
diminished
by
glutathione
(GSH),
commonly
overexpressed
in
tumors.
Herein,
a
breakthrough
strategy
involving
extracellular
vesicle
(EV)
mimetic
nanovesicles
(NVs)
encapsulating
iron
oxide
nanoparticles
(IONPs)
and
β-Lapachone
(Lapa)
was
developed
amplify
intracellular
oxidative
stress.
The
combination,
NV-IONP-Lapa,
created
through
serial
extrusion
from
ovarian
epithelial
cells
showed
excellent
biocompatibility
leveraged
magnetic
guidance
enhance
endocytosis
cancer
cells,
resulting
selective
generation
Lapa
catalysis
NADPH
quinone
oxidoreductase
1
(NQO1).
Meanwhile,
the
released
IONPs
ionization
under
acidic
conditions
triggered
conversion
of
•OH
Fenton
reaction.
Additionally,
process
eliminated
GSH
tumor,
further
amplifying
designed
NV-IONP-Lapa
demonstrated
exceptional
targeting,
facilitating
MR
imaging,
enhanced
suppression
without
significant
side
effects.
This
study
presents
promising
NV-based
drug
delivery
system
exploiting
CDT
against
NQO1-overexpressing
tumors
augmenting
intratumoral
Язык: Английский
The Neuromuscular Disorder Mediated by Extracellular Vesicles in Amyotrophic Lateral Sclerosis
Current Issues in Molecular Biology,
Год журнала:
2024,
Номер
46(6), С. 5999 - 6017
Опубликована: Июнь 14, 2024
Amyotrophic
lateral
sclerosis
(ALS)
represents
a
neurodegenerative
disorder
characterized
by
the
progressive
loss
of
both
upper
and
lower
motor
neurons,
resulting
in
muscular
atrophy
eventual
paralysis.
While
much
research
has
concentrated
on
investigating
impact
major
mutations
associated
with
ALS
neurons
central
nervous
system
(CNS)
cells,
recent
studies
have
unveiled
that
pathogenesis
extends
beyond
CNS
imbalances,
encompassing
dysregulation
other
tissues
such
as
skeletal
muscle.
Evidence
from
animal
models
patients
supports
this
broader
perspective.
Skeletal
muscle,
once
considered
solely
an
effector
organ,
is
now
recognized
possessing
significant
secretory
activity
capable
influencing
neuron
survival.
However,
precise
cellular
molecular
mechanisms
underlying
detrimental
effects
observed
muscle
its
structures
remain
poorly
understood.
Additionally,
emerging
data
suggest
extracellular
vesicles
(EVs)
may
play
role
establishment
function
neuromuscular
junction
(NMJ)
under
physiological
pathological
conditions
wasting
regeneration
muscles,
particularly
diseases
like
ALS.
This
review
aims
to
explore
key
findings
about
involvement
ALS,
shedding
light
potential
contributions
EVs
their
possible
application
for
design
biosensors.
Язык: Английский
Extracellular Vesicles as Potential Biomarkers in Addictive Disorders
Biochemistry (Moscow),
Год журнала:
2024,
Номер
89(11), С. 1970 - 1984
Опубликована: Ноя. 1, 2024
Язык: Английский
Early life exposure to deltamethrin impairs synaptic function by altering the brain derived extracellular vesicle proteome
Molecular & Cellular Proteomics,
Год журнала:
2024,
Номер
unknown, С. 100902 - 100902
Опубликована: Дек. 1, 2024
Pyrethroid
pesticides
have
been
associated
with
neurodevelopmental
disorders
including
attention
deficit
hyperactivity
disorder
(ADHD)
and
autism
spectrum
(ASD).
While
behavioral
effects
of
pyrethroid
exposure
previously
reported,
the
underlying
mechanisms
remain
unclear.
Here,
we
hypothesized
that
to
deltamethrin
(DM),
a
widely
used
pesticide
known
for
its
neurotoxicity
during
early
developmental
stages,
induces
brain
dysfunction
through
alterations
in
brain-derived
extracellular
vesicle
(BDEV)
signaling.
Using
well-established
rodent
model
life
DM
within
recommended
no
observable
effect
level,
isolated
BDEVs
from
postnatal
30-day-old
vehicle-exposed
(control)
DM-exposed
mice
using
differential
sucrose
density
gradient.
Following
ZetaView
nanoparticle
tracking
electron
microscopy
characterization,
quantitative
mass
spectrometry-based
proteomics
revealed
89
differentially
expressed
proteins
(DEPs)
exposed
animals
compared
control
BDEVs.
Bioinformatic
analysis
identified
convergence
DEPs
on
pathways
mitochondrial
function
synaptic
plasticity.
PKH67-green
conjugated
derived
either
or
were
bilaterally
injected
intracerebroventricularly
into
naïve
adult
mice,
distribution
labeled
was
verified
prior
field
recording
experiments.
Strikingly,
long-term
potentiation
(LTP)
at
CA3-CA1
hippocampal
synapses,
functional
correlate
learning
memory,
intact
BDEVs,
but
absent
receiving
mice.
Notably,
exogenously
delivering
LRRTM1,
one
found
disrupts
transmission
CA1
neurons
consistent
impaired
LTP.
Thus,
regulated
signaling
represents
novel
mechanism
neurotoxicity.
Язык: Английский
Specific Extracellular Vesicles, Generated and Operating at Synapses, Contribute to Neuronal Effects and Signaling
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(10), С. 5103 - 5103
Опубликована: Май 7, 2024
In
all
cell
types,
small
EVs,
very
abundant
extracellular
vesicles,
are
generated
and
accumulated
within
MVB
endocytic
cisternae.
Upon
fusion
exocytosis
with
the
plasma
membrane,
EVs
released
to
space.
central
nervous
system,
release
of
neuronal
was
believed
occur
only
from
surface
body
dendrites.
About
15
years
ago,
cisternae
were
shown
exist
function
at
synaptic
boutons,
terminals'
pre-
post-synaptic
structures
essential
for
canonical
neurotransmitter
release.
Recent
studies
have
revealed
that
peculiar
in
many
respects
heterogeneous
respect
other
EVs.
The
distribution
effect
their
specific
molecules
found
critical
sites
distribution.
role
could
consist
modulation
or
a
distinct,
non-canonical
form
neurotransmission.
Additional
roles
still
not
completely
known.
future,
additional
investigations
will
clarify
pathology,
concerning,
example,
circuits,
trans-synaptic
transmission,
diagnosis
therapy
diseases.
Язык: Английский