CRISPR/Cas12a-based biosensors for environmental monitoring and diagnostics
Environmental Technology & Innovation,
Год журнала:
2024,
Номер
34, С. 103625 - 103625
Опубликована: Апрель 4, 2024
Contaminants,
such
as
nucleic
acids
or
toxic
small
molecules,
threaten
both
human
health
and
ecosystems
when
they
infiltrate
the
environment.
The
precise
highly
sensitive
identification
of
contaminants
holds
paramount
importance
across
diverse
domains,
including
safeguarding
food
integrity,
facilitating
clinical
diagnostics,
monitoring
environmental
conditions.
Traditional
methodologies,
encompassing
spectroscopy,
chromatography,
sequencing,
metagenomics,
have
conventionally
served
pivotal
roles
in
detection
processes.
Nevertheless,
these
methods
encountered
recurring
challenges
related
to
sensitivity,
specificity,
portability.
This
review
focuses
on
groundbreaking
CRISPR/Cas12-based
biosensors.
These
biosensors
leverage
incredible
precision
programmability
CRISPR/Cas
system
recognize
specific
targets.
Here,
we
comprehensively
assess
fundamental
mechanisms
that
enable
detection,
ranging
from
guide
RNA
design
collateral
cleavage.
versatility
CRISPR/Cas12
becomes
evident
through
their
applications.
applications
encompass
medical
safety,
monitoring.
transition
conventional
ultimately
represents
a
significant
milestone
contaminant
detection.
By
incorporating
molecular
biology,
nanotechnology,
bioinformatics,
potential
reshape
landscape
water
CRIPSR-Cas
diagnostics
is
transformative
technology
paves
way
for
safer
healthier
future
environment
life.
Язык: Английский
Tumor Microenvironment‐Selective Sol–Gel Mineralization of ROS‐Responsive Stretchable and Conductive Hydrogel
Advanced Functional Materials,
Год журнала:
2024,
Номер
34(37)
Опубликована: Апрель 11, 2024
Abstract
Cancer
cell‐triggered
sol–gel
transformation
of
mineralized
hydrogel
(PAA‐MnO
2
)
is
designed
as
a
facile
strategy
for
cancer
detection
by
manipulating
the
mineralization
process
in
presence
cells.
The
polyacrylic
acid
(PAA)
with
calcium
phosphate
via
carboxyl‐Ca
2+
complex
initially
inhibited
incorporation
reactive
oxygen
species
(ROS)‐sensitive
manganese
oxide
(MnO
polymer
dots
(PDs).
In
this
system,
can
be
induced
after
cleaving
MnO
into
Mn
high
ROS
levels
cells,
forming
PAA‐MnO
and
resulting
naked‐eye
system
monitoring.
Naked‐eye
monitoring
ROS‐responsive
performed
using
circulator
device
containing
circulating
cells
to
discriminate
(HeLa,
PC‐3,
B16F10)
from
normal
(CHO‐K1).
With
PDs,
not
only
provides
physical
(stretchability,
viscosity)
but
also
fluorescence‐recovery
electroconductivity
changes
at
different
cancer‐cell
concentrations
(10
4
–10
6
mL
−1
),
including
distinct
strain–pressure
responses
that
wirelessly
monitored
smartphones.
Furthermore,
vivo,
experiments
suggest
formed
tumor‐bearing
mice
owing
its
excellent
ROS‐scavenging
activity
tumor
site,
confirmed
SOD2
gene‐expression
analysis.
Thus,
unique
approach
potentially
enable
simple
effective
future
point‐of‐care
diagnostics.
Язык: Английский
CRISPR-based electrochemical biosensors: an alternative for point-of-care diagnostics?
Talanta,
Год журнала:
2024,
Номер
278, С. 126467 - 126467
Опубликована: Июнь 28, 2024
Язык: Английский
Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Proteins System-Based Biosensors
Biosensors,
Год журнала:
2025,
Номер
15(3), С. 155 - 155
Опубликована: Март 2, 2025
High-sensitivity
and
high-specificity
biodetection
is
critical
for
advancing
applications
in
life
sciences,
biosafety,
food
safety,
environmental
monitoring.
CRISPR/Cas
systems
have
emerged
as
transformative
tools
biosensing
due
to
their
unparalleled
specificity,
programmability,
unique
enzymatic
activities.
They
exhibit
two
key
cleavage
behaviors:
precise
ON-target
guided
by
specific
protospacers,
which
ensures
accurate
target
recognition,
bystander
activity
triggered
upon
binding,
enables
robust
signal
amplification.
These
properties
make
highly
versatile
designing
biosensors
ultra-sensitive
detection.
This
review
comprehensively
explores
recent
advancements
system-based
biosensors,
highlighting
impact
on
improving
performance.
We
discuss
the
integration
of
with
diverse
readout
mechanisms,
including
electrochemical,
fluorescent,
colorimetric,
surface-enhanced
Raman
scattering
(SERS),
so
on.
Additionally,
we
examine
development
integrated
systems,
such
microfluidic
devices
portable
leverage
technology
point-of-care
testing
(POCT)
high-throughput
analysis.
Furthermore,
identify
unresolved
challenges,
aiming
inspire
innovative
solutions
accelerate
translation
these
technologies
into
practical
diagnostics,
food,
environment
safety.
Язык: Английский
Advances and applications of biosensors in pulmonary hypertension
Respiratory Research,
Год журнала:
2025,
Номер
26(1)
Опубликована: Апрель 15, 2025
Pulmonary
hypertension
(PH)
is
a
serious
disease
characterized
by
elevated
pulmonary
artery
pressure,
with
its
prevalence
and
incidence
continuously
increasing,
posing
threat
to
the
lives
of
many
patients
worldwide.
Due
complex
etiology
PH
lack
specificity
in
clinical
manifestations,
there
currently
effective
specific
methods
for
early
diagnosis
practice.
Biosensors
hold
significant
promise
detection,
therapeutic
monitoring,
prognostic
evaluation,
personalized
treatment
PH,
owing
their
rapid,
sensitive,
highly
selective
characteristics.
The
rapid
development
various
types
biosensors,
such
as
electrochemical
optical
microfluidic
wireless
combined
use
nanomaterials,
makes
accurate
detection
PH-related
biomarkers
possible.
Despite
broad
application
prospects
biosensors
field
challenges
remain
terms
sensitivity,
selectivity,
stability,
regulation.
This
article
reviews
main
pathophysiological
mechanisms
commonly
used
principles
summarizes
progress
research
well
current
challenges,
order
promote
further
in-depth
biosensor
technology,
thereby
improving
effects
PH.
Clinical
trial
number:
Not
applicable.
Язык: Английский
Carbon dot-based treatment for bacterial pneumonia by promoting a PI3K-mediated M1 polarization of macrophages
Journal of Nanobiotechnology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Апрель 26, 2025
As
the
incessant
emergence
of
drug-resistant
bacterial
strains,
pneumonia
poses
a
serious
threat
to
public
health
worldwide.
There
is
an
urgent
need
explore
unconventional
therapeutic
strategies.
Carbon
dots
are
usually
designed
directly
kill
bacteria,
however,
carbon
that
enhance
anti-infection
function
immune
cells
rarely
reported.
In
present
study,
CDots
were
synthesized
with
ascorbic
acid
and
polyethyleneimine,
exhibiting
outstanding
biocompatibility.
Functionally,
exhibited
well
impact
on
induced
by
gram-positive
multidrug-resistant
Staphylococcus
aureus
(MRSA)
or
gram-negative
Klebsiella
pneumoniae
(K.
pneumoniae)
in
mice.
Utilizing
vitro
models
macrophages
infected
MRSA
K.
pneumoniae,
we
discovered
augmented
M1
polarization
macrophages,
subsequently
enhancing
their
survival
activity
phagocytosis
bactericidal.
Further
investigations
through
molecular
dynamics
simulations
experiments
validated
bind
catalytic
subunit
(PIK3CD)
phosphoinositide
3-kinase
(PI3K),
resulting
inhibition
PI3K/AKT/mTOR
signaling
pathway.
Moreover,
crucial
domain
for
binding
was
located
amino
acids
752-787
PIK3CD.
summary,
exerted
protective
effect
targeting
PIK3CD
fostering
PI3K-mediated
macrophages.
These
findings
not
only
reveal
new
role
treatment
pneumonia,
but
also
provide
potential
targets
future
Язык: Английский
Semiconducting Polymer Dot-based Wireless Electrochemical Aptasensor for Detection of Aging-related TGF-β1 and IL-6
Analytica Chimica Acta,
Год журнала:
2025,
Номер
1360, С. 344139 - 344139
Опубликована: Май 2, 2025
Язык: Английский
Coenzyme-A-Responsive Nanogel-Coated Electrochemical Sensor for Osteoarthritis-Detection-Based Genetic Models
Gels,
Год журнала:
2024,
Номер
10(7), С. 451 - 451
Опубликована: Июль 10, 2024
An
electrochemical
sensor
sensitive
to
coenzyme
A
(CoA)
was
designed
using
a
CoA-responsive
polyallylamine-manganese
oxide-polymer
dot
nanogel
coated
on
the
electrode
surface
detect
various
genetic
models
of
osteoarthritis
(OA).
The
responded
abundance
CoA
in
OA,
causing
breakage
MnO
Язык: Английский
Electrochemical and Fluorescence MnO2-Polymer Dot Electrode Sensor for Osteoarthritis-Based Peroxisomal β-Oxidation Knockout Model
Biosensors,
Год журнала:
2024,
Номер
14(7), С. 357 - 357
Опубликована: Июль 22, 2024
A
coenzyme
(CoA-SH)-responsive
dual
electrochemical
and
fluorescence-based
sensor
was
designed
utilizing
an
MnO2-immobilized-polymer-dot
(MnO2@D-PD)-coated
electrode
for
the
sensitive
detection
of
osteoarthritis
(OA)
in
a
peroxisomal
β-oxidation
knockout
model.
The
CoA-SH-responsive
MnO2@D-PD-coated
interacted
sensitively
with
CoA-SH
OA
chondrocytes,
triggering
electroconductivity
fluorescence
changes
due
to
cleavage
MnO2
nanosheet
on
electrode.
can
detect
immature
articular
chondrocyte
primary
cells,
as
indicated
by
significant
increase
resistance
control
medium
(R24h
=
2.17
MΩ).
This
also
monitored
cells
presence
acetyl-CoA
inducers,
such
phytol
(Phy)
sodium
acetate
(SA),
2.67,
3.08
MΩ,
respectively),
compared
that
medium,
demonstrating
efficiency
towards
concentration.
Furthermore,
recovery
observed
owing
cleavage,
particularly
Phy-
SA-supplemented
media.
transcription
levels
OA-related
anabolic
(Acan)
catabolic
factors
(Adamts5)
chondrocytes
confirmed
interaction
between
Additionally,
integration
wireless
sensing
system
provides
inline
monitoring
via
smartphone,
which
potentially
be
used
rapid
diagnosis.
Язык: Английский